gradient to separate 1kDa proteins - (Apr/10/2009 )
Hi all, i would know at what % should can i make a gel gradient to separate 2 proteins that differs each other only for 1kDa...
it depends on the actual mw of the proteins.
1kDa difference is more significant in a small protein than a large one.
alessandro on Apr 10 2009, 09:40 AM said:
You may not be able to. Westerns aren't necesarily exact, you usually need to give a band about a 5kDa margin of error.
You will probably need 2D resolution.