Re-dissolving of precipitated protein?? - (Aug/30/2009 )
I recently heat precipitated (95oC 5 mins) one of the protein (monoclonal antibody) I am working on, now I need to re-dissolve it, for some further analysis?? Could some suggest me methods/protocol for the same…?
Thanks a lot
Proteins aggregate upon heat denaturation. This leads to precipitation. A useful way to denature protein without precipitation is to perform denaturation with guanidine hydrochloride or another denaturant. This chemical will denature your protein and prevent protein aggregation, in most cases. With this your protein will not precipitate.