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IGg bands on SDS PAGE - (Apr/09/2010 )

Hi all,

After IGg purification do I have to see one band or two bands(l ight and heavey chains)?

-bluebird-

bluebird on Apr 9 2010, 12:15 PM said:

Hi all,

After IGg purification do I have to see one band or two bands(l ight and heavey chains)?



Well, that depends whether you are running reducing or non-reducing gels. If reducing, you will see 2 bands. If you run non-reducing gels you might still see some bands corresponding to the light and heavy chains but the major band should correspond to the intact antibody.

Hope this helps.

-almost a doctor-

yes it helped so much.

Many Thanks Doctor

-bluebird-