Is there a such thing that would allow me to generate 2 individual proteins, but - (May/30/2013 )

their expression in the same cell causes them to come together? i.e. a molecular tool for creating endogenous fusion proteins

Do you mean like subunits of proteins, or are you talking about joining proteins together as a single transcript?

For both, the answer is sure - you can express more than one protein inside any cell, so you should be able to do this OK. For mammalian proteins there are even "cell free" systems such as the rabbit reticulocyte lysate system.

Single transcript can be done by cloning as I am sure you know.

bob1 on Fri May 31 05:24:10 2013 said:

What I'm asking is there like a short linker peptide (peptide #1) that can be expressed on one transgene, and another peptide (peptide #2) that can be expressed on another transgene, thereby only allowing the two proteins to come together when both transgenes are coexpressed in the same cell.

i.e. Transgene #1 = Protein-of-Interest #1 + peptide #1

Transgene #2 = Protein-of-interest #2 + peptide #2

AH, kind of like Biotin/streptavidin - you could try calmodulin and calmodulin recognition peptide tag.

bob1 on Sat Jun 1 09:37:46 2013 said:

Awesome, thanks. I know you've probably been at this stuff for a while, but is there a go to resource you usually use when trying to find out things like this.

Sorry, not that I am aware of. I usually just do a google search or two.