Protein extraction from tissue - (Mar/03/2010 )
Hi there,
I need to extract proteins from a tiny muscle fibre. I'm tending to use the T-PER tissue protein extraction reagent. But I don't know how i could perform the homogenization. Obviously, a hand-hold glass homogenizer won't do since my muscle tissue is very small. Any suggestion on this plz? like sonication?
Many thx,
Jo
-Zhe-
you can get small (0.5 ml) glass homogenisers from Kontes glassware?, if that is any help.
Edited to add link to 0.5 ml Dounce.
-bob1-