Protocol Online logo
Top : New Forum Archives (2009-): : Cell Biology

Wound healing (scratch) assay - extracellular matrix protein substrate (Aug/12/2010 )

I currently want to establish the wound-healing assay (scratch assay) in our lab. I am looking for a suitable ECM substrate (laminin-5?) for my cell culture dishes to be coated with! I work with cancer cell lines of epithelial origin.

hope some can help,

thx in advance


I think you can perform the scratch assay without coating if you are working on adherent cancer cell lines.