Brief vortexing for reconstitution of recombinant proteins? - (Sep/27/2012 )
I have a very simple question.
Generally, vortexing is not recommended while reconsituting lyophilized recombinant proteins.
Can a brief vortexing (e.g. 10 sec. in 1xPBS supplemented with 0.1% BSA) result in a significant loss of the proteins' bioactivity? Did anyone experience problems because of vortexing?
- goals go global -
vortexing can cause denaturation of the protein due to physical stress (and also, possibly, oxidation).
it can be performed if you can cause a swirling effect rather than bumping.