Non-denaturing buffer to extract nuclear protein - (Apr/02/2014 )
Can anybody suggest me a non-denaturing buffer composition for extracting nuclear protein? I will be using that protein for the purpose of isolating a multi-protein complex. Will inclusion of 110 mM KCl in the lysis buffer disrupt the protein -protein interaction among the subunits? Please help me. Thanks for your patience.
The key here is probably detergents - avoid SDS containing buffers, triton will pop the nuclear membrane. You could also look at mechanical disruption.