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Non-denaturing buffer to extract nuclear protein - (Apr/02/2014 )

Can anybody suggest me a non-denaturing buffer composition for extracting nuclear protein? I will be using that protein for the purpose of isolating a multi-protein complex. Will inclusion of 110 mM KCl in the lysis buffer disrupt the protein -protein interaction among the subunits? Please help me. Thanks for your patience.


The key here is probably detergents - avoid SDS containing buffers, triton will pop the nuclear membrane.  You could also look at mechanical disruption.