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effect of lysozyme on gst-fusion protein - (Jul/09/2011 )

hi everybody,

i have a gst fusion protein with a thrombin cutting site. I just do not understand if it is normal to see the cleaved form of my gst and target protein as early as bacterial lysis? i found the cleaved form of my protein in the supernatant after bacterial lysis. is it possible? or is it because i incubated my bacterial pellet for too long with lysozyme?


couldn't be due to lysozime which its ligand is the peptidoglycane cell wall!!!