ammonium precipitation of Proteins - (Mar/19/2011 )
I want to precipitate the protein twice with amoinum sulfate. First 45 % and then 100 %. When I will do 45% initial amount of ammonium sulfate is 0. I will add the required am. sulfate and then centrifuge the solution and dicard the pellet and take the supernatent. This supertatent I want to precipitate to 100 %. For this step initail ceoncetration of am. sulfate is 0 or 45 % to count the amount of am. sulfate for second 100 % precipitation.
-samita-
for the second step the starting concentration is 45%.
Regards,
p
samita on Sat Mar 19 20:30:48 2011 said:
I want to precipitate the protein twice with amoinum sulfate. First 45 % and then 100 %. When I will do 45% initial amount of ammonium sulfate is 0. I will add the required am. sulfate and then centrifuge the solution and dicard the pellet and take the supernatent. This supertatent I want to precipitate to 100 %. For this step initail ceoncetration of am. sulfate is 0 or 45 % to count the amount of am. sulfate for second 100 % precipitation.
-pDNA-
Thanks
pDNA on Sun Mar 20 06:22:22 2011 said:
for the second step the starting concentration is 45%.
Regards,
p
samita on Sat Mar 19 20:30:48 2011 said:
I want to precipitate the protein twice with amoinum sulfate. First 45 % and then 100 %. When I will do 45% initial amount of ammonium sulfate is 0. I will add the required am. sulfate and then centrifuge the solution and dicard the pellet and take the supernatent. This supertatent I want to precipitate to 100 %. For this step initail ceoncetration of am. sulfate is 0 or 45 % to count the amount of am. sulfate for second 100 % precipitation.
-samita-