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IgG SDS-PAGE - (Jun/19/2010 )

Hi all-

I recently ran a SDS-PAGE gel of a monoclonal antibody (+ 2X SB) and after developing the western, I noticed there were 3 bands on my film. I know I should see a heavy and light chain, but does the third (and largest) band indicate that some of the antibody was not denatured?



Maybe this can help.