Protocol Online logo
Top : New Forum Archives (2009-): : Molecular Cloning

Which epitope tag to use for ChIP? - (Jan/09/2014 )

From your experience, which epitope tag worked the best for ChIP? I am going to tag my protein but I also would like that epitope tag to be suitable for ChIP. I have thought about Flag, Myc, HA, V5, His, or maybe you have another tag that worked for you. Please explain why the epitope tag worked poorly or well. 
 
Note: I understand the antibody plays a major role.
 
Thanks!
 

-Epigeneticist-

Has anyone tagged their protein with HA or Flag and done ChIP PCR or ChiP seq? Comments? Suggestions?

-Epigeneticist-