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column chromatography - elution buffer for proteins (Oct/09/2010 )

What antimicrobial agents can be used in order to prevent growth of microbs in eluent buffer for gel filtration of proteins7

-caterina-

depends on the protein. some are compatible with small amounts of sodium azide.

-mdfenko-

Kathon is also widely used and relatively safe (for mammals). Deadly to fish, however, so be careful of disposal.

-phage434-