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protein phosphorylation status in sample buffer - (May/09/2011 )

will my phosphorylated GST tagged peptide stay phosphorylated if stored in regular denaturing sample buffer overnight before running the gel or is it better to run the gel immediately?

-xenopus 1-

Assuming that it is phosphorylated to begin with--ie, you added phosphatase inhibitors and what not to your lysis buffer--it should be fine to run the next day


in general, it is not recommended to store proteins in sample buffer; but if, it would be useful to store in a phosphate containing buffer which, however, is rarely found in sample buffers

-Inmost sun-