bacteria lysis - E coli lysis for recombinant protein extraction (Oct/11/2009 )
There is a commercially avilable buffer BPERII from Piece in which I am adding Protease inhibitor cocktail III and benzonase nuclease for the lysis of the E coli cells. But inspite of using BPERII, can I add some non ionic detergent to the Lysis Buffer(20mM Tris HCl, 5mM Imidazole, 500mM NaCl) to get the same type of cell lysis?
-NK22-
Hi,
I do the bacteria lysis with PBS and tween20+ DTT and sonication. Some time I add sarcosyl. But the lysis buffer is PBS-T. DTT or sarcosyle is for solubility. Detergent work well for me with sonication to get ride of DNA. So yes I think you can use it.
Sara
NK22 on Oct 11 2009, 05:23 PM said:
There is a commercially avilable buffer BPERII from Piece in which I am adding Protease inhibitor cocktail III and benzonase nuclease for the lysis of the E coli cells. But inspite of using BPERII, can I add some non ionic detergent to the Lysis Buffer(20mM Tris HCl, 5mM Imidazole, 500mM NaCl) to get the same type of cell lysis?
-saraarasus-