small proteins - (Dec/18/2012 )
Hi, I want to observe some small proteins (MW=15 to 25kD) on SDS-PAGE. These are multipule proteins. Do you have recommendations how I can make them stains better, or seperate better to observe?
Thanks a lot!
High percent gel is the primary thing you should use. Long gels will help with the separation too.
you can use a tris-tricine sds gel. it gives great resolution of low molecular weight proteins.
you can use colloidal coomassie to get a more intense stain. you can silver stain (it's 50-100x more sensitive than coomassie).