Protein measurements in 2% Triton X samples - (Jan/11/2012 )
do any of you know what techniques can be used to determine protein quantity in cell lysates that contain 2% triton x. I have lysed my cells in lysis buffer containing 2% triton, because I am looking at a membrane bound protein (LC3) by WB.
Bradford etc. reagents are reacting very! good with Triton X, and the reagent gets saturated very fast by Triton.
the bca protein assay is compatible with up to 5% triton x-100 (1% other tritons)