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cys rich protein expression - (Mar/07/2010 )

I am trying to express protein that has 33 cysteins out of total of 117 amino acids. this protein binds to cadmium. I am expereincing problems in expressing it. Its giving weak expression and sometimes none. I was wondering if it is being oxidized while expressing and thus removed by the proteolytic enzymes. kindly tell me ways to tackle the problems.

please help me out.

-z0z-

Can you give more details? What protocol are you using for expression, detection, purification... etc?

-Another Jake-