Protein isolation and lack of sop's - (Feb/24/2013 )
We are a group of university students from leiden (The netherlands )
We are all Microbiologists so whe do know very few about proteomics.
we have 1 bacteriologist 1 mycologist and all round microbiologist.
and i'm the medical microbiologist and biochemist
we have to do an assigement to purify the enzyme peroxidase out of horse radish.
for this experiment we need several SOP's
1) extraction ( SOP made by myself)
3) BCA analysis i used that sop form santa cruiz university
4) peroxidase test we have one dated 1994 are there any recent sop's?
6)dialysis we have that found already
i know about Size exclusion but not what TSK2000 is so please anyone can you help 3 students out?
9) the last
we have that done before i found half of that protocol because UAS Leiden gave us that before.
any hints ,tips or links to good SOP's we will be very happy
so please help us out
with kind regards
student :Medical microbiology
University of Appleid Science Leiden
you can download handbooks on affinity chromatography (including con-a) and gel filtration from this webpage:
ge life sciences
you can find an assay for hrp and links to more information at this page:
the attached ms word document will give more information on precipitation with ammonium sulfate.
tsk2000 is a specific gel filtration column for use on hplc apparatus. tsk is the company and 2000 is the packing type. this page will download the tsk brochure on sec:
hope these help.