Protein precipitation from RIPA buffer to perform ELISA - (Sep/07/2012 )
I have extracted protein from tissue in RIPA buffer. I have got good yeild but I understood from the Kit protocol/ company's technical support that RIPA buffer cannot be used for doing ELISA's. Could anyone please tell me a working protocol to precipitate protein out of RIPA buffer so that I could use it for ELISA's. .
Many thanks in advance.
you can dialyze away the problematic components of the ripa.
Thank you. Could you please elaborate or send me a protocol to do it. Thanks in advance
mdfenko on Mon Sep 10 16:09:21 2012 said: