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denaturing proteins in gel - (Mar/19/2009 )

I'm interested in determining if my protein is running as a dimer (or higher). I have a peptide antibody that works on denatured protein but when I ran a non-reducing gel, the antibody did not recognize the protein. I would like to be able to denature the protein in the (non-reducing) gel after it has run to determine if the protein is running as an oligomer. Does anyone know how I would go about denaturing the protein in the gel?


you could try soaking in sds and 2-me (or dtt).

or you could try transferring then soaking in sds and 2-me to denature on the membrane.