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how to make a recombinant protein with signal peptide - (Nov/30/2011 )

I want to express a part of a protein, but I want to include the signal peptide.

What is the general procedure of making a recombinant protein, with addition of a N-term signal peptide? Should I include the peptide coding sequence in the 5'-primer? But it is very long (~60bp).

Thanks a lot!


Hola, the general procedure is subcloning your protein in a vector which will contains signal peptide. There are some in the pET system for bacteria and in any other expression system that you choice as baculovirus for instance that I know. Buena suerte