Usage of EST data for non-model species in proteomics - (Sep/30/2009 )
I am working on two dimensional gel electrophoresis-based proteomics on a non-model species (i.e. genome not sequenced).
Recently, a team generated the cDNA library of my studied species. May I know if I can use these EST information to help identifiy the proteins that could not be matched after MALDI TOF/TOF?
Thank you, appreciated.
Sure, there shouldn't be a problem with it, be aware that RNA doesn't always equal protein though.
bob1 on Oct 1 2009, 07:45 AM said:
May I know what program should I use in order to carry out the search?