protein precipitation without phenol/chloroform for dna extraction? - (Jul/16/2009 )
we've been using 4M guanidine thiocyanate to lyse cells (whole blood) for dna extraction. what is the best way to precipitate proteins from this lysis solution without using phenol/chloroform?
thanks in advance!
Salt 'em out: add 10 M Na Acetate to final concentration of 2 M.