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Rcovery of proteins from gels - (Mar/06/2009 )

I wonder if anyone knows of a way to recover proteins from an electrophorsis gel?????



Cut the band out, put it in a dialysis tube with a cut off less than the size of yr protein and fill with buffer, put it in a tank and apply voltage. The protein will move out of the gel but stay inside the dilysis tubing, take it out, concentrate and use.


-T C-

or you could mash the gel and wash out the protein with buffer (and a centrifuge, and you could use a centrifugal filter instead of pelleting and decanting) then concentrate.