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c1q ? Protein A? Or Protein G for immune complex detection? - immune complex detection (Aug/10/2010 )

There are some efficient magnetic beads coated with Protein A Or Protein G for Immunoprecipitation. However, I read somewhere said that C1q should be used in the measurement of human immune complex.

Do you have any information about this?

-newborn-

Ya c1q can be used for the measurement of human immune complex because C1q-bearing immune complexes induce IL-8 secretion in human umbilical vein endothelial cells (HUVEC) through protein tyrosine kinase- and mitogen-activated protein kinase-dependent mechanisms: evidence that the 126 kD phagocytic C1q receptor mediates immune complex activation of HUVEC

For further queries: biology test papers

Regards
Rupam Mittal

-Rupam-

Rupam on Tue Aug 24 23:29:49 2010 said:


Ya c1q can be used for the measurement of human immune complex because C1q-bearing immune complexes induce IL-8 secretion in human umbilical vein endothelial cells (HUVEC) through protein tyrosine kinase- and mitogen-activated protein kinase-dependent mechanisms: evidence that the 126 kD phagocytic C1q receptor mediates immune complex activation of HUVEC

For further queries: biology test papers

Regards
Rupam Mittal


Thanks for your reply, do you think which protein (C1q, Protein A or Protein G) should be used to precipitate immune complex?

-newborn-