Protein A-agarose or A-sepharose for immunoprecipitation? - (Apr/30/2010 )
I used dynabeads protein G for with anti-rabbit antibody to immunopurify my protein. but, i couldnt' immunoprecipitate!
I'm wondering if its question of protein G, and i want to try Protein A. I found protein A-agarose aliqot and i want to try to check if i could pull down!
i would like to ask if protein A-sepharose is better than A- agarose in my case (to purify my protein from cerebral lysate)!!
thanks for help
sepharose is ge healthcare's (nee pharmacia) brand name for agarose. protein a-sepharose and protein a-agarose are virtually the same.
ah ok,!! i see!
because one collegue that A-sepharose is more used and better than agarose!
thanks for your answer!
well, there are some aging and quality control issues but for your purpose it should make no significant difference (i prefer sepharose to generic agarose).