Meaning of Recombinant Protein - (May/10/2010 )
The usual definition of recombinant protein is a protein which is produced from recombinant DNA. Such protein usually tagged with something like His-tag, GST-tag, or any other protein.
I wonder if a protein produced from recombinant DNA, but totally similar to the native one (doesn't tagged with anything), e.g. antibiotic resistance enzyme produced from a certain plasmid as selection marker, do we also call it "recombinant protein"?
Kidd on May 10 2010, 07:15 AM said:
I wonder if a protein produced from recombinant DNA, but totally similar to the native one (doesn't tagged with anything), e.g. antibiotic resistance enzyme produced from a certain plasmid as selection marker, do we also call it "recombinant protein"?
yes of course. IMO recombinant proteins are non-native proteins produced using molecular techonology to clone and express. I don't think having tag or not makes a difference.
Yes as we produced these proteins manually by changing the composition and putting one's protein in some other host to produce new and different protein.
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Regards
Rupam
Basic recombinant DNA techniques require digestion of DNA vector with restriction enzymes, which are molecular scissors that cut DNA at specific locations. DNA from the organism of interest is also digested in separate tubes, with the same restriction enzymes. Two DNA are then mixed together and joined this time by using an enzyme called DNA ligase that intact, double-stranded DNA molecule. This structure can be introduced into cells of Escherichia coli, where DNA is copied billions of times.