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can beta-catenin be used as a house keeping protein? - (Feb/20/2009 )

i ran out of actin and my new shipment has not arrived as yet...

just wondering if beta-catenin can be my housekeeping protein for a western blot (protein lysate from 3t3-l1 cells). i know i

any response would be awesome...

-medstudent1-

beta-cantinin is regulated by many stimulation. you can try tubulin or GAPDH if you have

-robby-

robby on Feb 21 2009, 06:58 PM said:

beta-catenin is regulated by many stimulation. you can try tubulin or GAPDH if you have

-robby-

If this protein will not be modified after your experiments, then yes.

if this protien levels are changed due to your treatment, then might be use another gene.

-scolix-