protein concentration per well - (Jul/27/2010 )
some one told me that protein is loaded with respect to the size of the wel, let say 10 micrograms per one cm, is it true or not. What i assume was 10 micrograms per micro liter.
hope to get answer soon
i have never heard of any such concept... u load watever amount u want in each well depending on ur application taking care u dont under or overload the protein!!!
what amount u can load depends on the protein concentration and the well dimention.. tats it!!!
Thanks...may u b blessed wid great achievements in life.
In continution with SDS PAGE, can we reuse the electrode buffer which is incontact with the samples in stacking gel.
well if u are a very poor loab then u may reuse it.. but i wud not reuse the running buffers!!!
Protein and DNA/RNA are quite frequently loaded based on the width of the lane, if you load too much you get a blob with some smearing for a band, if you load too little, you won't be able to see the band. The quantities people usually say to load are typically for 8 mm lanes.
You can reuse buffer about once, but it won't work very well as the electrolytes run out.