purify protein from native gel - (Jul/13/2013 )
Hi all,
Wanted to know if anyone has experience purifying proteins from native PAGE gels for downstream applications like EMSA? I was thinking about using invitrogen's NativePage Bis-Tris Gel system and cutting out my band of interest and eluting from gel. Is this nuts?
MSB
-Kaire76-
not at all. we've used that procedure for some difficult to purify proteins. we prepare prep size and thickness gels to load large amounts of protein, cut and stain a lane and use that to line up the band of interest.
we used the resulting purified proteins to inoculate rabbits.
-mdfenko-