Protein concentration of a labelled membrane protein - (Jan/27/2013 )

Hopefully somebody could help me out...

I have labelled a membrane protein with a probe (atto655) and I want to know the protein concentration.

As the protein is a membrane protein I have detergent present (tritonx100) and so can't use Abs at 280 nm.

The dye absorbs between approx 550 and 700 so I can't use a bradford or BCA assay.

Is there any other assay I could use in this circumstance?

-Vinnie-

2D quant kit can be used, just check-

http://biology200.gsu.edu/core/Manuals/2D%20Quant%20protocol.pdf

-neuron-

neuron on Mon Jan 28 04:25:18 2013 said:

2D quant kit can be used, just check-

http://biology200.gs...nt protocol.pdf

Thank you!

I think this will work for me!

-Vinnie-