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Protein concentration of a labelled membrane protein - (Jan/27/2013 )

Hopefully somebody could help me out...

I have labelled a membrane protein with a probe (atto655) and I want to know the protein concentration.

As the protein is a membrane protein I have detergent present (tritonx100) and so can't use Abs at 280 nm.

The dye absorbs between approx 550 and 700 so I can't use a bradford or BCA assay.

Is there any other assay I could use in this circumstance?


2D quant kit can be used, just check-


neuron on Mon Jan 28 04:25:18 2013 said:

2D quant kit can be used, just check- protocol.pdf

Thank you!

I think this will work for me!