Localization of kanamycin resistance protein - (Aug/02/2010 )
does anyone know where the neomycin phosotransferase (nptII, the common kanamycin resistance found in many cloning vectors) is localized in E. coli?
Is it a cytoplasmic protein or is located in the periplasmic space? Does anyone know relevant literature on this topic?
Many thanks for your help!
okay, i will answer my question
According to brenda its secreted into the periplasmic space: