FPLC and Protein Chromatography - Resins, FPLC and protein chromatography (Aug/17/2009 )

Hello,

I use FPLC quite a bit. I was just wondering if there were any good alternatives/competitors to GE Healthcare (aka Pharmacia) resins and FPLC columns. I've had fair experience with their systems, but their products are rather pricey and the over pressuring on some columns (especially when loading samples) is rather annoying.

So I want to look for some alternatives. Can you help?

Try Waters. They are pretty well known for this sort of thing, though mostly focussed on HPLC and LC sort of stuff.

What types of separations are you thinking of? Google the chemistry type and you should see whoever else makes similar resins, columns etc. You could also go to www.biocompare.com for a general search engine.

One brand that I used to use was POROS perfusion beads. Very high flow rates very sharp bands for the ion exchange columns.

you can check out bio-rad:
medium pressure columns

Hello again,

Thank you for the suggestions.
Sorbitech does columns, but not common resins.
http://www.sorbtech.com/Chromatography/LPM...conolineColumns

I'm just doing ion exchange and gel filtration for various proteins. HPLC sounds similar to FPLC, but I'm not sure that you can interchange the columns...

Luria Bertani on Aug 19 2009, 11:26 PM said:

The main difference is the pressure that the system can handle. You can use HPLC columns on FPLC systems and vice versa, as long as you are aware of the baseline pressures of each system. What system are you using?

Oh, thank you.

I'm using AKTA FPLC manufactured from GE Healthcare.

For gelfiltration and most large scale ion exchange columns that usually only involves low pressure operation, we always buy empty columns and resions and pack ourselves.

Luria Bertani on Aug 21 2009, 09:19 AM said:

the akta is a biocompatible hplc. it can be used for both hplc and fplc columns.

mdfenko on Sat Aug 22 00:55:12 2009 said:

the akta is a biocompatible hplc. it can be used for both hplc and fplc columns.