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Do protein purification under partially denatured condition - (Oct/24/2013 )

Hello, everyone,

I am wondering if there is anyone here has experience with purification under partially denatured condition, like add 2-4M Urea?

I want some support about this. Could you give me some materials about this, or some kind of web link?


Thank you very much.


Dear Hellen,


Not sure if this helps, but there is a handbook for "protein sample preparation" which you can download from link below:


On top there are a few handbooks which might be beneficial as a reference for your research work as well.


Hope this helps.




-Adrian K-