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Do protein purification under partially denatured condition - (Oct/23/2013 )

Hello, everyone,

I am wondering if there is anyone here has experience with purification under partially denatured condition, like add 2-4M Urea?

I want some support about this. Could you give me some materials about this, or some kind of web link?

 

Thank you very much.

-Hellen-

Dear Hellen,

 

Not sure if this helps, but there is a handbook for "protein sample preparation" which you can download from link below:

 

http://www.gelifesciences.com/webapp/wcs/stores/servlet/catalog/en/GELifeSciences-us/service-and-support/handbooks

 

On top there are a few handbooks which might be beneficial as a reference for your research work as well.

 

Hope this helps.

 

Rgs,

Adrian

-Adrian K-