IgG control for immunopercipitation - (Mar/13/2009 )
Does anyone know why when performing immunopercipitation experiments IgG is used as a loading control as opposed to traditional controls (i.e. actin, GAPDH,tubulin)
You want a control that has similar characteristics to your target protein. IgG doesn't polymerize, whereas actin does. IgG is a glob whilst tubilin is long and stringy. Lastly, other Igs would polymerize (IgM, IgA).
Does that help.