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IgG control for immunopercipitation - (Mar/13/2009 )

Does anyone know why when performing immunopercipitation experiments IgG is used as a loading control as opposed to traditional controls (i.e. actin, GAPDH,tubulin)


-Brian Wynter-

You want a control that has similar characteristics to your target protein. IgG doesn't polymerize, whereas actin does. IgG is a glob whilst tubilin is long and stringy. Lastly, other Igs would polymerize (IgM, IgA).

Does that help.