Native PAGE w. Western protein marker - (Oct/01/2009 )
I'd like to run a native PAGE followed by Western. What native protein standard I can use for that? I need it to be stained, but I can't find anything on a net. Does anybody knows something? Thanks for advice!
Try NativeMark from invitrogen
Hi! I saw this marker, but it is NOT stained! I need something I could blot on the membrane along with my protein of interest and see the marker on membrane as well.
bob1 on Oct 2 2009, 01:33 AM said:
prestained markers are denatured (i'm not aware of any prestained native gel standards). if you want native markers then you will have to transfer and stain the membrane with ponceau s to see them.
Thank you. This is confirmation of what I was suspecting.
mdfenko on Oct 2 2009, 08:35 PM said: