HiTrap Heparin HP for purification of DNA binding proteins. - (Jun/27/2012 )
I have bought "HiTrap Heparin HP" for purification of DNA binding proteins.
This the first time that I am going to do such a purification and I do not have any knowledge and experiences,
Do you have a protocol for using this colums for purification of DNA binding proteins?
As I am going to use these purified proteins for next steps such as DNA-Affinity Chromatography,
what should I use as Elution buffer?
My concern is NaCl in Elution buffer of "HiTrap Heparin HP" for dissociating the proteins from Heparin, because it interferes with DNA-Affinity Chromatography. I use NaCl in my elution buffer of DNA-Affinity Chromatography to dissociate proteins from bounded Oligos on the beads.
check the response to your post at the biotechniques forum.