protocol to transfer protein on transfer membrane fluoride bind - please tell me how to transfer proteins from sds page (Mar/10/2011 )
please tell me how to transfer proteins from sds page to transfer membrane fluoride bind ( serva) with help of semi dry blotting equipment
mdfenko on Thu Mar 10 20:03:17 2011 said:
do you have the manual that came with the semi-dry transfer unit? different brands have different recommended methods.
in the meantime, read this handbook:
Hi, I tried to open the pdf but i couldn't.
Can u email it to my address firstname.lastname@example.org
i can't access cleaver's website. if you can then you should be able to download the manual for your semi-dry unit. that will give you the information that you need.
usually methanol is 10-20% in the transfer buffer.