Immunodepletion of high abundance protein - (Oct/27/2009 )

Hi there,

I have problems in the reproducibility of the human serum depletion step. Most of the times, high level of albumin was found in low abundant protein (by SDS-PAGE). I use MARS column to deplete 30 samples until now.

Thanks

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Anasyida

have you considered igy based depletion?

check this brochure from beckman-coulter and phenomenex: