mutation in secreted protein - (Mar/02/2009 )

Hi,

I'm measuring the expresson of a serum proetin (a secreted protein in serum) using ELISA. My question is how do i chek for mutation in such a protein?

SF_HK on Mar 2 2009, 05:44 PM said:

SF_HK on Mar 2 2009, 05:44 PM said:

Do I have to check the mutation in the tissue sample corresponding to that serum sample showing dysregulated expression?

Hi,
What kind of mutation do you expect to affect secretion of the protein? A mutation affecting expression level, or protein properties?

expression level

SF_HK on Mar 2 2009, 05:34 PM said:

Expression level can be affected by multiple factors, for example, changes in promoter sequence, epigenetic modifications, upstream regulatory factors, ...