sensitive protein quantification assay - (May/13/2009 )
I am extracting membrane protein (tagging and extracting) from cells and I am facing problem quantifying them. I use BCA and my standard curve is ok. I use 1mg/ml BSA in my respective buffer as my standard. and use diff concs. I also tried eluting my protein using less eluting buffer, but didnt help. I think BCA is not very sensitive in my case. I also made sure all components being used are compatible with the assay.
Please suggest help!!
Concentrate sample? Verify performance/sensitivity of the assay with control material.
have you tried the bradford protein assay?