Is CNBr activated Sepharose good for binding IgG? - (Nov/12/2010 )
I want to purify a antigen, and i got its rabbit form antibody IgG
I dont have DMP/DSS and it will take few weeks to order and for its arrived
but i got some CNBr activated Sepharose , i know that it will attached to the amino group of the protein
will it be effective for binding IgG without losing the activity of IgG?
Thanks for any advice!!!
cnbr-sepharose will bind the antibody but its orientation will be random. because of this not all of the bound antibodies will be active.
so do u suggest i should do cross linking with DSS/ DMP?
if you are referring to crosslinking the antibody to protein A (or G or A/G) agarose, then yes. this way all of the antibodies should be active for capture of your antigen.