will high-speed centrifuge rupture protein G? - (Jul/22/2010 )
last time I centrifuged my protein G at 2400rpm. but Sigma protocol for my new anti-flag slurry gel suggests over 9000rpm. isn't this too high to break the beads and release bound antibodies?
it depends upon which material the beads are made. but if sigma tells you to spin at 9000 rpm(? not g?) then use what they say.