co-ip of endogenous and tagged proteins - (Jul/15/2013 )
hello everyone,
sorry for my simple basic question, but for studies and experiments, will it be enough to perform co-ip from endogenous proteins? why is it sometimes necessary to transfect his or gst-fused protein and then perform co-IP? is it only for validation? thank you...
-soymilk14-
Generally speaking, antibodies for the tag (ie 6His or FLAG or GFP) are more specific than the antibody against the targeted endogenous protein. Therefore, you should have less non-specific interactions with the antibody and then the IP should be cleaner.
-Tom M-