why the bands migrate lower in the gel EMSA? - as I increase protein concentration (Jan/10/2010 )
Hi all
My first post.
question i have in regards to my EMSA is why the bands migrate lower and lower in the gel as I increase protein concentration?
best,
-stipprn-
Where are the lower bands relative to the probe alone? Are they lower or higher? Also, do you use a RNAse/DNAse inhibitor in your reactions?
-kannan75-