BCA microplate assay with 595 nm filter? - (Apr/06/2016 )
I want to do a BCA protein microplate assay (pierce) but our plate reader only has a 595 nm filter. The manufacturer of the BCA kit advises to use a 562 nm filter, or at most a 590 nm filter. Has anyone successfully used a 595 nm filter for this assay?
I used two different BCA kit before, CreativeBiomart and Beyotime. Here are their products descriptions:
In the PDF, OD562nm is best choice. But 540-595nm are also suitable for their products. I'm not sure whether 595nm also suitable for your kit.