Protein localization - Protein localization (Feb/05/2010 )
Hi, I;m new to working with proteins and westerns etc; I have a mutant which has a N terminus truncation of a helicase protein and I need to study the localization of this protein on the replication fork. Can some one give me some hints as to how I should et about starting on this. I think I can try to do an IP with it via an antibody; but how to do that or any other suggestions.?
Cellular fractionation - e.g. nuclear fractionation, followed by western. You could also do it by immunofluorescence. Both methods require you to have antibodies against the mutant and normal proteins.