Best gel percentage to separate 24 and 36 kDa proteins - (May/09/2012 )
Hi there,
I was wondering what gel percentage would be best to achieve the greatest separation between a protein of 24 kDa and GAPDH (36 kDa), since I subsequently wanted to cut the PVDF membrane in between. I don't have any beta-Actin antibodies right now.
you should get adequate separation with a 10 or 12% gel. test run with standards to ensure that you will get the resolution you require.
you can use pre-cast gradiant gel.
http://www.piercenet.com/browse.cfm?fldID=F230FBCE-11F9-4334-8EC7-14F7CD64CE52
I have used Precise Protein Gels, 4-20%, 10-Well Cat#25204.
They are easy to use and quick and perfect.
They sell this pre-cast gel in three different well capacity. 25ul, 30ul and 50ul.
Babak
memari on Wed May 9 16:57:17 2012 said:
you can use pre-cast gradiant gel.
http://www.piercenet...C7-14F7CD64CE52
I have used Precise Protein Gels, 4-20%, 10-Well Cat#25204.
They are easy to use and quick and perfect.
They sell this pre-cast gel in three different well capacity. 25ul, 30ul and 50ul.
Babak
should not use a gradient that long if you want to separate 24 and 36kDa with enough distance to cut apart from the membrane.