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Best gel percentage to separate 24 and 36 kDa proteins - (May/09/2012 )

Hi there,

I was wondering what gel percentage would be best to achieve the greatest separation between a protein of 24 kDa and GAPDH (36 kDa), since I subsequently wanted to cut the PVDF membrane in between. I don't have any beta-Actin antibodies right now.

-steinpdh-

you should get adequate separation with a 10 or 12% gel. test run with standards to ensure that you will get the resolution you require.

-mdfenko-

you can use pre-cast gradiant gel.

http://www.piercenet.com/browse.cfm?fldID=F230FBCE-11F9-4334-8EC7-14F7CD64CE52

I have used Precise Protein Gels, 4-20%, 10-Well Cat#25204.

They are easy to use and quick and perfect.

They sell this pre-cast gel in three different well capacity. 25ul, 30ul and 50ul.


Babak

-memari-

memari on Wed May 9 16:57:17 2012 said:


you can use pre-cast gradiant gel.

http://www.piercenet...C7-14F7CD64CE52

I have used Precise Protein Gels, 4-20%, 10-Well Cat#25204.

They are easy to use and quick and perfect.

They sell this pre-cast gel in three different well capacity. 25ul, 30ul and 50ul.


Babak

should not use a gradient that long if you want to separate 24 and 36kDa with enough distance to cut apart from the membrane.

-mdfenko-