DNA Methods, Protocols and Troubleshootings

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DNA Related Discussions

Finding the mass of digested DNA by electrophoresis - (reply: 1)
Staining DNA and blotting - (reply: 1)
different Ct values for RNaseP gene in human genomic DNA - (reply: 1)
genomic DNA extraction from tissue - (reply: 1)
What casues dark zone in middle of DNA band on agarose gel? - (reply: 7)
DNA ladders on a northern blot ?.. - (reply: 1)
cell free DNA (cfDNA) - (reply: 1)
cell-free DNA from urine - (reply: 1)
Problems for DNA agarose gel visualization with a gel documentation system - (reply: 9)
DNA - (reply: 1)
Difficult DNA extraction-collagen mutant nematode refusing to give up its DNA - (reply: 3)
Pyura DNA extraction - (reply: 1)
"DNA Laser Printing" - Cambrian Genomics - Homework - (reply: 1)
BAC DNA purification - (reply: 4)
DNA extraction - (reply: 2)
DNA precipictation at -80 went thick - (reply: 2)
Plasmid dna size - (reply: 3)
Free software for DNA fingerprint analysis - GUI based - (reply: 1)
How clean do I need my DNA to be? - (reply: 1)
We need a way to see microRNA and DNA in vitro for reverse aging - (reply: 15)
Problematic DNA Extraction - (reply: 1)
Recombinant DNA vaccine production - (reply: 9)
DNA extraction with phenol-chloroform - DNA stuck in phenol layer? - (reply: 1)
Using RNA minikit for DNA extraction - (reply: 4)
Purification of linearized AT-rich DNA for transfection - (reply: 4)
DNA decontamination chemistry - (reply: 1)
DNA extraction from hair - issues on nanodrop - (reply: 1)
The true monomers making RNA and DNA ? - (reply: 1)
DNA concentration - (reply: 1)
DNA Extraction from Tissue Slides - (reply: 1)
Splicing DNA fragments - (reply: 2)
HTP DNA and RNA extraction options??? - (reply: 2)
QPCR with genomic DNA from blood - (reply: 2)
Arranging DNA sequences in text file - (reply: 2)
Addition of DNase after RNA extraction - (reply: 4)
PROTOCOL FOR LINEARIZED DNA - (reply: 1)
DNA extraction from FFPE tissue - (reply: 2)
Calculate DNA amount - (reply: 1)
Cloning - Miniprep plasmid contaminated by gDNA? - (reply: 1)
DNA Macroarray - (reply: 1)
Using SDS for DNA electrophoresis - (reply: 3)
Migration of circular DNA vs circular/crosslinked peptide in gels - (reply: 2)
Will lipofection of DNA work in post mitotic cells - (reply: 1)
Overcoming methylation of bacterial DNA which inhibits transformation and genome - (reply: 6)
DNA extraction from saliva - (reply: 1)
Of the nature of DNA - (reply: 3)
*NO* DNA eluted from plasmid prep - Why? - (reply: 12)
Inhibition of PCR amplification of bacterial genomic DNA by RNA - (reply: 1)
DNA extraction from blood using SDS and phe:chl - (reply: 4)
How to extract Biotinylated DNA from low melting agarose without use of phenol e - (reply: 1)
Any DNA/RNA elements I can include into transgene to delay expression? - (reply: 2)
DNA spin column to get RNA? - (reply: 1)
DNA amount for RE reaction - (reply: 3)
DNA dilution 1, 2, 4, 8, 10 X - (reply: 1)
dna extraction - (reply: 4)
Epigenetics Workshop - DNA Methylation Data Analysis in Leipzig, Germany (15-17 - (reply: 1)
Ethanol precipitation of DNA - (reply: 21)
Comparing DNA fragments using restriction map - (reply: 1)
DNA concentration no spectrophotometer - (reply: 4)
Designing a simple assay for global DNA methylation - (reply: 6)
result of too much DNAse? - (reply: 2)
Pseudomonas aeruginosa DNA extraction - (reply: 8)
Composition of Buffer AL, Qiagen DNA minikit? - (reply: 1)
Isolation of genomic DNA following RNA extraction with Qiazol? - (reply: 1)
How to select promoter for resistance gene in linear/naked DNA "knockout" - (reply: 2)
Low RNA yield in dual RNA/DNA extraction - (reply: 1)
dna isolation - (reply: 1)
DNA isolation protocol needed!!!! - (reply: 1)
dna isolation from bacterial cultures - (reply: 4)
Amplification problem in DNA isolated after ChIP experiment, - (reply: 1)
Visualization of DNA:RNA hybrid on TBE-UREA GEL - (reply: 8)
Can´t get rid of DNA contamination in RNA - (reply: 2)
PCR DNA bands too large on agarose gel - (reply: 1)
How can DNA purity be checked? (original title "PROTOCOLS") - (reply: 1)
Bacteria grow on antibiotic selective plate but plasmid extracted showed no dna - (reply: 6)
Thermo scientific T4 DNA ligase product help - (reply: 3)
RNA extraction and DNase treatment - (reply: 1)
RNA extraction and DNase treatment - (reply: 2)
Second generation DNA synthesis column - (reply: 1)
concentrate DNA - (reply: 3)
DNA SDS-PAGE electrophoresis - (reply: 6)
Low plasmid yield after Miniprep, DNA in the first wash flow through (PB) - (reply: 1)
All I get is supercoiled DNA... - (reply: 5)
DNA shows on Nanodrop but not on electrophoresis - (reply: 2)
Simple DNA analysis software for PC? - (reply: 2)
MNase assay - how to quantify DNA - (reply: 3)
Genomic DNA quantification using the NanoDrop - (reply: 3)
Determine DNA sequence expression in cell line - (reply: 1)
Separaing double stranded dna - (reply: 1)
Methods to Sequencing "Unknown" DNA - (reply: 2)
Low 260/230 ratios from DNA purification question - (reply: 1)
EZ DNA Methylation-Lightning Kit Incomplete Conversion‏ - (reply: 5)
Quantuitative staining of RNA and DNA? - (reply: 4)
DNA loading dye contents - (reply: 3)
ChIP: Inconsistent reverse crosslinking and DNA purification result - (reply: 2)
DNA disappearing after restriction - (reply: 10)
Decontaminating DNA - Effective Means? - (reply: 4)
first time running DNA polyacrylamide TBE gel - (reply: 4)
DNA gel elctrophoresis problem - (reply: 3)
DNA extraction problems. - (reply: 3)
No sequencing of DNA methylation - (reply: 6)
Transforming dna from agarose gel - (reply: 3)
question on calculation DNA amount - (reply: 5)
DNA exraction from parasites - (reply: 1)
Why would you use DNase as a control in dot blot assay confirming viroid import? - (reply: 1)
ethanol precipitation plasmid DNA - (reply: 3)
Missing DNA Target and Effect on Non-Specific Amplicons - (reply: 1)
DNA extraction from dissociated embryos - (reply: 2)
DNA stability in STE with proteinase K - (reply: 1)
Cell lysis and DNA quantification - (reply: 1)
What is the protocol to remove RNA contamination (Using RNase) from DNA? - (reply: 8)
Bacterial DNA extraction/purification from animal tissue - (reply: 1)
PCR of bisulfite converted DNA is now producing a smear? Previously produced a s - (reply: 2)
DNA damage or genomic instability: How to evaluate them? - (reply: 1)
chk effect of cell line genes on normal dna - (reply: 1)
Nanodrop machine error message when measuring plasmid DNA concentration - (reply: 3)
DNA extraction from Vermiculite - (reply: 8)
Can you store DNA in loading dye buffer short term? - (reply: 2)
DNA Global Methylation with Epigentek kit-having trouble - (reply: 1)
Quick method for DNA extraction using lysis - (reply: 4)
DNA virus purification from agarose failed! - (reply: 7)
Any real difference between DNA purification kits? - (reply: 9)
DNA sequencing for GC rich sequence - (reply: 3)
Subsequent DNA and siRNA transfection - (reply: 9)
DNA cleanup after linearization, before transformation? - (reply: 3)
A modified method for blunt end DNA cloning!!!!!!! - (reply: 2)
Which method for DNA purification b4 cloning - (reply: 4)
gDNA still presents even after DNase treatment - (reply: 6)
DNA Extraction from buccal swabs - (reply: 7)
Dna isolation from fungi - (reply: 2)
PCR and sequencing of genomic DNA - (reply: 5)
Purified DNA fragments are bigger than unpurified - (reply: 2)
Genomic DNA Isolation from Soft Agar Colonies - (reply: 3)
DNA extraction from endive (low yield + peak at 220 nm) - (reply: 4)
Low RNA quantity after on column DNAse digestion - (reply: 1)
enviromental water samples DNA isolation - (reply: 2)
Can I concentrate already extracted DNA with an extraction kit!? - (reply: 9)
PCR inhibitor in template DNA - (reply: 3)
PCR from protozoa DNA - (reply: 3)
Midiprep from mini prep DNA - (reply: 2)
Alternative phenol-free method for DNA extraction - (reply: 2)
Live cell sort DNA stain permeabilization - (reply: 1)
DNA Quantification of PCR Products - (reply: 2)
Plasmids DNA on filter paper - (reply: 1)
How to qualitatively measure DNA methylation of gene promoter - (reply: 1)
Loss of DNA after restriction digestion cleanup - (reply: 2)
Testing for DNA degradation prior to Southern Blot Experiment - (reply: 2)
Weird fragments in my gDNA - (reply: 2)
HELP - Adaptor ligation to DNA not working! - (reply: 1)
Dissolving DNA Oligos - (reply: 2)
Using 10,000 rpm instead of 14,000 for DNA extraction with Promega kit - (reply: 2)
DNA from frozed cells??? - (reply: 2)
DNA Gel purification column issues/mistakes? What's your process? - (reply: 6)
does DNA methylation outside promoter region affect gene Transcription/expressio - (reply: 1)
faint dna band after pcr purification - (reply: 1)
Storage life of plasmid DNA in water - (reply: 5)
Weirdest thing with DNA dye and agarose gel - (reply: 4)
How to design primer to amplify genomic DNA? - (reply: 3)
How many fibroblasts for 1ug DNA? - (reply: 2)
Plasmid DNA digestion problem - (reply: 3)
PCR products sizes and DNA ladder - (reply: 7)
No Band With Large DNA (BAC) - (reply: 1)
DNA Liquid into DNA pellet - (reply: 4)
DNA isolation from whole blood + clotting??!! - (reply: 2)
need help (DNA) - (reply: 2)
free online DNA & plasmid editing tool - (reply: 1)
Plant DNA analysis by UV spectrophotometer indicates there is DNA in my sample, - (reply: 1)
Manipulation of target DNA fragments - (reply: 1)
Smeared DNA ladder - (reply: 3)
DNA extraction problem - strange bands - (reply: 4)
Nanodrop vs. picogreen: 100fold difference DNA yield from buccal swab!? - (reply: 9)
atom replacement in DNA - (reply: 1)
Loading dye in DNA - (reply: 2)
DNA extraction impurities - (reply: 23)
phenol/chloroform extraction of genomic DNA - (reply: 6)
Phenol-Chloroform Extraction (DNA) - (reply: 5)
genomic DNA extraction on silica-based membrane - (reply: 1)
DNA extraction from the gel - low yield - (reply: 2)
Help!! Double band in genomic dna - (reply: 1)
DNA extraction (Phenol chloroform Vs NaOH) - (reply: 5)
Reconstituting plasmid DNA from blotting paper - water vs. TE buffer - (reply: 6)
DNA extraction , phenol , ice . plz help ! - (reply: 3)
Dotted bands on my DNA agarose electrophoresis gel. - (reply: 2)
I dissolved the DNA in 1XTAE instead of TE buffer, will it affect ligation? - (reply: 1)
Frameshift mutation in DNA sequencing? - (reply: 1)
identification of transcription factors binding to DNA - (reply: 1)
Gel purification (DNA) - (reply: 1)
DNA contamination from adenovirus afted DNase treatment - (reply: 2)
Labeling DNA with Biotin - (reply: 2)
DNA extraction kits - (reply: 3)
Sigma Imprint DNA methylation quantification assay kit - (reply: 3)
How to determine the location of integrated DNA in stable cell lines - (reply: 2)
Viral contamination in DNA purification columns - (reply: 1)
Information about the use of DNA diluted in Real Time PCR - (reply: 6)
Running Plasmid DNA on Agarose Gel (Banding Pattern) - (reply: 1)
PCR failing for transformed plant DNA but not for Agro - (reply: 3)
problems with gDNA doing real time PCR in yeast - (reply: 2)
Question about DNA purification from a restriction digestion reaction - (reply: 1)
Simultaneous RNA and DNA transfection? - (reply: 2)
standard curve using genomic dna - (reply: 1)
DNA storage degradation - (reply: 1)
Qiagen midi prep DNA yield - (reply: 3)
Sodium Acetate pH 5.2 or 5.5 for DNA precipitation - (reply: 1)
Column for removing short DNA digestion products - (reply: 6)
What is the membrane made of in DNA purification kits - (reply: 3)
Removing Introns from a DNA sequence - (reply: 1)
DNA extraction from a partially-eaten fruit - (reply: 5)
Quantifying bisulfite-converted DNA (pre- and post-pcr) and direct sequencing - (reply: 2)
How to get rid of DNA contamination in RNA prep - (reply: 4)
DNA sequencing - (reply: 2)
DNA ladder does not resolve well - (reply: 4)
Qubit quantification too low yield DNA - (reply: 4)
AcONa 3M switched by AcOK 3M during DNA precipitation! - (reply: 1)
Will long exposures of DNA to UV (302nm) alter sequencing data? - (reply: 4)
types of dna cloning methods - (reply: 2)
dna left on thermocycler - (reply: 2)
Northern blot DNA probes - (reply: 1)
How to avoid RNA contamination in DNA samples:? - (reply: 1)
RNA contamination in DNA samples - (reply: 2)
Global DNA hypomethylation assay - (reply: 1)
removing foreign DNA - (reply: 4)
Limit of detection for salmon gDNA? - (reply: 7)
Doubt: genomic dna library construction - (reply: 3)
Drugs that cause DNA double strand breaks - (reply: 1)
SBI Minicircle DNA Kit -- No colonies -- ligation / transformation problem - (reply: 8)
No DNA after gel extraction - (reply: 9)
DNA isolation from nuclei - (reply: 5)
DNA in 1water:0,1TE at 37 degrees - (reply: 1)
Plasmid DNA contamination - (reply: 5)
Problems with plasmid DNA extraction - (reply: 3)
percentage DNA fragmentation - (reply: 1)
DNA laddering - (reply: 1)
Protocol for DNA fragmentation Analysis - (reply: 2)
nicked linear DNA - (reply: 5)
DNA isolation - (reply: 4)
Plasmid DNA - (reply: 3)
Scaling up PCR to get more DNA - (reply: 5)
Cleaning DNA - (reply: 2)
Buying a DNA synthesizer- advice, tips? - (reply: 1)
Genomic DNA and plasmid DNA isolation - (reply: 3)
Extraction of DNA from termite gut flagellates - (reply: 7)
autoclaving tubes for DNA extraction - (reply: 2)
Genomic DNA extraction from filters - (reply: 3)
accidentally used denatured ethanol for DNA and RNA - what to do? - (reply: 4)
Phenol Chloroform Isoamyl Alcohol (25:24:21) DNA purification - (reply: 3)
overlap in the DNA sequence - (reply: 6)
Low DNA concentration for sequencing - (reply: 5)
No DNA after PCR product purification - (reply: 9)
DNA extraction from whole blood - (reply: 6)
DNA extraction using qiagen kit - (reply: 4)
Role of sodium acetate in DNA extraction/precipitation - (reply: 7)
using sybergreen to quantify DNA and assess its quality - (reply: 1)
mtDNA to nDNA Ratio gDNA - qPCR - (reply: 1)
High Molecular Weight DNA extraction and PFGE - (reply: 2)
the cultivation of genetics and DNA of Kazakh people - (reply: 3)
non redundant DNA - (reply: 4)
Grass genomic DNA extraction - (reply: 1)
Template DNA for PCR- Concrentration or Volume?? - (reply: 1)
cell free DNA and maintaining stability - (reply: 3)
Sensitive, Consistent and Reproducible Method(s) for Extraction of gDNA from Low - (reply: 2)
PCR DNA Concentration - (reply: 1)
Quantify total DNA in small cell lysate...? - (reply: 1)
Quantify DNA in gel electrophoresis bands - (reply: 7)
What happen to my animal DNA extraction? - (reply: 12)
Homozygote DNA but Heterozygote RNA? - (reply: 2)
Slime formation in DNA extraction - (reply: 12)
Help: Best way to disrupt blood cells to generate blood debris and gDNA to be us - (reply: 4)
How double stranded DNA becomes single stranded in LAMP assay? - (reply: 3)
DNA Methylation and genomic variation - (reply: 2)
Trouble with DNA purity (A260/A280) - (reply: 3)
Straight bands on DNA gel - (reply: 3)
Whole Genomic DNA and when to cut - (reply: 2)
DNA size band increased after gel electrophoresis with Quaigen fit - (reply: 4)
Recipe for TKM1 and TKM2 buffer for DNA isolation from Whole blood - (reply: 1)
qPCR with Genomic DNA - (reply: 1)
DNA extraction from whole blood using phenol chloroform - (reply: 2)
No bands in PCR after DNase treatment - (reply: 4)
Can anyone vouch for a protocol to get good gDNA from fungi? - (reply: 2)
Successful Transformation->Getting Colongy--> Getting DNA after miniprep - (reply: 9)
Which DNA strand is recommended - (reply: 1)
Qiagen column eluted DNA gives higher concetration than the original sample - (reply: 10)
System to label DNA - (reply: 6)
Why do only some nuclear lysis buffers create DNA 'goop' - (reply: 1)
DNA ligation and trasformation - (reply: 6)
AA/DNA Sequence Splitter and PDB to FASTA converter - (reply: 2)
Quality of Bisulfite Converted DNA - (reply: 2)
how to find out the DNA seuence of a human gene from online database? - (reply: 1)
too much DNA on agarose gel - (reply: 10)
PCR not working for 5 Aza treated DNA - (reply: 3)
Fast, cheap and dependable DNA extraction protocol for students? - (reply: 3)
isolating DNA from cells in suspension - (reply: 5)
DNA polymerase for GC rich template? - (reply: 5)
Replication of the Plasmid DNA - (reply: 6)
DNA extraction - PCR Problem - (reply: 3)
different PARTS different DNA ,,, - (reply: 4)
DNA extraction from non-viable PBMCs - (reply: 2)
Threw ribosome or DNA - (reply: 4)
Choose Ribosome or DNA - (reply: 3)
Storing Many DNA Samples - (reply: 2)
Question about isolating DNA from the blood sample - (reply: 2)
how can I remove the pigments during DNA extraction? - (reply: 1)
how to remove brown pigment in Sargassum DNA isolation? - (reply: 4)
Is there any software that can predict DNA binding proteins based on input DNA s - (reply: 2)
isolating DNA from mouse tails and subsequent problems with pcr - (reply: 7)
Restriction enzyme - PCR sheep blood gDNA - (reply: 5)
Viral genomic DNA isolation ? - (reply: 3)
Porportion methylation of bisulfite DNA changes with time? - (reply: 4)
615 or 660? Average MW of a DNA base pair - (reply: 3)
DNA EtOH/Sodium Acetate precip over the weekend...still good? - (reply: 2)
What happens to DNA in the presence of a chaotropic salt? - (reply: 3)
Quantify gDNA in buccal swab - (reply: 2)
A substitute for Triton-X100 in DNA extraction - (reply: 9)
Plasmid DNA at RT for a few days - (reply: 4)
Control primers for bisulfite-converted DNA - (reply: 3)
impurities after DNA extraction with promega kit!!!!!!&# - (reply: 6)
DNA amplification differences between samples & between regions (WGA & s - (reply: 2)
Low DNA concentration from mini-preps - (reply: 3)
lipofectamine 2000:DNA complex stability - (reply: 2)
Best method for extraction on low concentration environmental DNA? - (reply: 2)
Ethanol precipitation of DNA. How long...? - (reply: 3)
Genomic DNA extraction - how to quantify for PCR? - (reply: 9)
separate plant protoplast from bacteria‏ prior DNA extraction - (reply: 3)
DNA Kit Stability - (reply: 4)
DNA and siRNA transfection and cytotoxicity - (reply: 1)
suitable DNA extraction and sample freezing for MSP - (reply: 1)
PhiX174 DNA - (reply: 1)
Purity problems in DNA extracted from stool samples - (reply: 3)
DNA or cDNA? - (reply: 1)
Where can I purchase T2 or T4 phage genomic DNA? - (reply: 2)
Extraction of insect mitochondrial DNA - (reply: 2)
How many ug of DNA per ChIP is recommendable. - (reply: 5)
DNA extraction for MCF-7 and HeLa - (reply: 4)
About Methylated & Non-methylated pUC19 DNA Set - (reply: 1)
DNA extraction from Fin Clips - (reply: 4)
DNA pellet disappeared after ethanol wash - (reply: 3)
Checking DNase activity - (reply: 3)
DNA lost dramatically during storage - (reply: 5)
Synthesis of carrier RNA(poly-A) for DNA Extraction - (reply: 2)
Mycobacterium DNA Dilution for Quantification - (reply: 2)
Sterile Air Drying of DNA - (reply: 3)
DNA pellet - (reply: 2)
DNA Isolation from Mouse Tissue - (reply: 1)
no DNA fragment pull down in the first CpG island (MBD-seq) - (reply: 2)
problems with recovery of dna from gel and ligation - (reply: 5)
Chromosomal DNA? - (reply: 5)
DNA of mitochondria - - (reply: 1)
mitochondrial DNA - (reply: 7)
Effect of varying DNA concentration levels in methylation analysis - (reply: 2)
Dissolving DNA - finding better techniques - (reply: 12)
Cell Fixation for DNA flow cytometry in fibroblasts - (reply: 2)
What gel percent and Voltage is needed to separate 2680bp and 2600bp DNA nicely - (reply: 7)
Dilution of Stock DNA - (reply: 4)
Why do i get DNA of low concentrate after purification with kit - (reply: 9)
DNA sequencing after ligation! HELP! - (reply: 8)
Making DNA Linkers for cloning purposes - (reply: 1)
Quantify DNA before ChIP PCR - (reply: 3)
can we extract DNA from snap freeze tissue - (reply: 2)
About Hot Start Taq DNA Polymerases - (reply: 2)
Question about PCR - DNA or RNA as template - (reply: 4)
cloning short genomic DNA fragments - (reply: 1)
Incorporating RNAse A into DNA extraction - (reply: 2)
ethanol in final DNA sample - (reply: 1)
Uncut whole genomic DNA running too high - (reply: 1)
Primers amplify genomic DNA but not cDNA - (reply: 2)
Clean genomic DNA and plasmid DNA extraction from E.coli - (reply: 3)
How to extract DNA from small number of cells - (reply: 2)
Ethanol Precipitation ChIP DNA troubleshoot - (reply: 1)
digestion and labelling of large ammount of DNA - (reply: 2)
DNA extraction from varnished insect bodies - (reply: 2)
genomic DNA extraction from formalin fixed tissues (not embedded) for genotyping - (reply: 4)
Is the genomic DNA similar among different cell lines? - (reply: 4)
Degraded DNA from Mouse Tissues - (reply: 5)
How getting truncated DNA fragments? - (reply: 7)
Genotyping genomic DNA before DNase treatment? - (reply: 1)
How to concentrate DNA - (reply: 8)
annotate mRNA and gDNA seq, Identify 5' and 3' untraslated regions, Iden - (reply: 2)
SDS precipitation after adding DNA binding buffer for column purification - (reply: 1)
Recommended tamplate DNA concentration for qPCR - (reply: 2)
Plasmid prep - no DNA pellet after ethanol precipitation - (reply: 4)
How many fragments in plasmid DNA prep? - (reply: 1)
DNA extraction from Potato Chips - (reply: 6)
MW of PEG for Purification of DNA - (reply: 2)
Enough checks for gDNA contamination in qPCR? - (reply: 3)
HiTrap Heparin HP for purification of DNA binding proteins. - (reply: 1)
sample of choice for non-tumor DNA - (reply: 5)
DNA extration from bacteria using kits or conventional methods - (reply: 6)
DNA fragmentation - (reply: 1)
Denaturation of DNA blotted - (reply: 1)
very low concentraion of DNA from Bacteria - (reply: 3)
DNA dissappearing during SAP incubation - (reply: 3)
what is your DNA concentration after gel extraction? - (reply: 5)
gDNA Isolation from Human Breast Tissue - (reply: 2)
Saliva and GCF DNA purification - (reply: 1)
DNA sequence of Herpes - (reply: 2)
Remove HA Tag in DNA sequence! Help - (reply: 1)
DNA isolation/purification troubleshooting. - (reply: 5)
Colony PCR works but DNA yeild very low after miniprep - (reply: 2)
No bands appearing in DNA gel electrophoresis - (reply: 1)
Conversion of ng/ul of DNA to ng for a PCR reaction - (reply: 2)
Denatured ethanol and DNA preservation - (reply: 2)
DNA in methylation analysis - (reply: 3)
Shearing issue: always having unsheared DNA (Please help!) - (reply: 9)
multiple bands for genomic DNA extraction - (reply: 3)
Getting genomic DNA for PCR - (reply: 4)
I can´t obtain the amount of DNA required for ligation of blunt ends - (reply: 2)
Phase separation during DNA extraction - (reply: 5)
Digestion of Genomic DNA - (reply: 1)
Are hybridoma germline DNA and hyrbidoma cDNA sequences identical? - (reply: 2)
Repurifying DNA - (reply: 3)
How to dilute the template DNA for PCR - (reply: 7)
After I run my pcr on agarose the DNA is still in the well - (reply: 3)
unespecific band PCR from mouse genomic DNA - (reply: 9)
Extracting DNA from fruits and Vegetables - (reply: 8)
Does DNase work at 8 degree? - (reply: 1)
DNA quantification - (reply: 1)
Isolation of both DNA and RNA from the same plant tissue - (reply: 8)
Vertical agarose gel electrophoresis for DNA - (reply: 1)
I forgot treat my samples with proteinase K before the DNA extraction step - (reply: 1)
DNA Construction: HIV-1 Tat Gene - (reply: 9)
mitochondrial DNA extraction from avian blood -urgent - (reply: 5)
Cotransient Transfections DNA Requirements - (reply: 3)
Generating stable cell line with linear DNA - (reply: 3)
generating small DNA fragments with stick ends by annealing oligos? - (reply: 1)
Weird results for gDNA extraction... - (reply: 5)
precipitation of RNA after DNase treatment - (reply: 3)
Smear above plasmid dna - (reply: 3)
restriction digestion buffers contaminated with dnase - (reply: 3)
Can we use denatured DNA standards on RNA formamide gels - (reply: 2)
plotting DNA methylation profile vs TSS - (reply: 1)
No DNA after midiprep - (reply: 2)
problem in expressing A DNA binding protein - (reply: 6)
Troubleshooting for DNA purification - (reply: 1)
RNA immunoprecipitation DNase treatment - (reply: 4)
Weight of a single Lambda DNA. - (reply: 3)
cDNA or genomic DNA for over expression in Arabidopsis - (reply: 1)
PCR failure from yeast genomic DNA - (reply: 1)
failure of PCR from yeast genomic DNA - (reply: 1)
Problem: Cloning DNA shuffled band - (reply: 2)
troubles in DNA digestion - (reply: 7)
Does DNA have to be supercoiled for transient protein expression? - (reply: 4)
Colony lift hybridisation positive but clones are negative for insert DNA - (reply: 1)
How to clean extracted faecal DNA? (removing PCR inhibitors) - (reply: 1)
dna extraction from plant tissue infected with fungus - (reply: 1)
DNA Storage - (reply: 10)
Quiagen DNA extraction kit problem - (reply: 3)
DNA extraction from cerebrospinal fluid using trizol - (reply: 2)
Problem with restriction digestion of agarose-embedded DNA - (reply: 4)
RT-PCR inhibited after Turbo DNase treatment - (reply: 3)
isolation of genomic DNA from chicken blood in conventional method - (reply: 3)
DNA and RNA extraction - (reply: 6)
ChIP on episomal DNA - (reply: 2)
DNA ladder running faster than samples during agarose gel electrophoresis - (reply: 1)
isopropanol DNA precipitation - (reply: 1)
That is DNA breathing? - (reply: 4)
How to add restriction enzyme site into the DNA fragment - (reply: 3)
isolation of genomic bacterial DNA (bacillus) - (reply: 2)
DNA extraction and paraffin embedded tissue - (reply: 5)
PAGE for Separation of DNA - (reply: 3)
Template DNA (plasmid) concentration for PCR - (reply: 4)
How to determine the volume of lisis buffer and DNA extraction buffer when the a - (reply: 5)
How to find that my RNA is not contaminated with gDNA, before running Real-Time - (reply: 5)
No band after extracting DNA from bacillus subtilis - (reply: 1)
Bacillus subtilis DNA extraction protocol - (reply: 7)
dna sequencing time - (reply: 1)
Highly Variable DNA yield from Neutrophils lysed & sheared for ChIP - (reply: 7)
Ammonium acetate DNA extraction mistake - (reply: 1)
cell DNA content of cells grown on different scaffolds - (reply: 2)
no dna after extraction - (reply: 5)
Can I re-precipitate plasma DNA in TE? - (reply: 3)
restricted DNA from the enzymes Bam H1, Hind111, Eco R1 and Sau 3A fall in? - (reply: 1)
How do I insert a DNA sequence into a multiple cloning site and add restriction - (reply: 1)
Strange PCR result from genomic DNA - (reply: 3)
Protein contamination with DNA - (reply: 5)
Quantifying DNA from Buccal Swabs - (reply: 1)
help in digesting DNA fragment using restriction enzyme - (reply: 2)
DNA Electrophoresis and SB (sodium borate) Buffer - (reply: 3)
DNA methylation of a promoter region in different age groups - (reply: 2)
Dilution, Cell concentration & DNA content - (reply: 3)
RNAse treatment of genomic DNA - concentration and duration? - (reply: 1)
turbid DNA from maxiprep - (reply: 3)
DNA sequence data came back with lots of wrong bases - (reply: 5)
synthetic DNA - (reply: 1)
Isolating Genomic DNA from a small colony of cultured human cells - (reply: 1)
Help: I am loosing the DNA during PCR purification - (reply: 7)
PCR primer with no extra DNA end - (reply: 5)
Protein contamination - How to remove all protein from my DNA? - (reply: 3)
Extracting DNA from FACS sorted cells - (reply: 3)
Didint get any band during gel electrophoresis even though DNA was there - (reply: 5)
syto9 for DNA gel electrophoresis - (reply: 1)
Environmental DNA extractions - (reply: 2)
Help with DNA acrylamide gel - leaking and errors during gel running - (reply: 4)
In a pcr why doesnt the DNA reneal during annealing process ? - (reply: 2)
Difference between plasmid DNA extraction and genomic DNA extraction - (reply: 3)
Weird DNA electrophoresis result - (reply: 4)
Gooey DNA is protein samples after adding sample buffer - (reply: 1)
DNA extraction and blue dextran as a DNA carrier - (reply: 4)
DNA degradation (from human blood) - (reply: 3)
Getting Rid of DNA after Transcription - (reply: 1)
DNA migrate differently in agarose vs PAGE gel - (reply: 4)
DNA Concentration - (reply: 1)
PCR products form with DNA, but not with c-DNA - (reply: 2)
DNA polymerase: recombinant or native? - (reply: 3)
To isolate DNA, RNA and Protein from same culture flask for methylation study? - (reply: 2)
Bisulfite treatment of DNA follow by Sequencing - (reply: 2)
Help to construct luciferase DNA construct - construct luciferase DNA construct (reply: 2)
concentration - concentration of DNA (reply: 3)
Random digest of double stranded DNA - (reply: 1)
effect of DNA isolation and storage on methylation - (reply: 1)
nested PCR with low target DNA? - (reply: 2)
DNA precipitation - A general question about DNA precipitation (reply: 3)
Purification of DNA after MeDIP and WGA - (reply: 1)
DNA extraction of microalgae - microalgae (reply: 3)
DNA polymerase with single G 3'-end overhang activity - (reply: 3)
~200kb Plasmid DNA purification is not working - (reply: 1)
Plasmid DNA size - Plasmid DNA backbone size (reply: 1)
DNA probe labeling for EMSA; How? - Long probe, not oligonucletides (reply: 1)
DNA extract quality - (reply: 3)
Stacking gel for DNA PAGE - (reply: 2)
Newbie- choose DNA template - (reply: 1)
DNA extraction from liver - (reply: 3)
DNA extraction - we want to extract DNA from bone marrow smear (reply: 2)
Ligating Incompatible DNA fragments - Ligating Incompatible DNA fragments (reply: 3)
Lambda DNA ligation - (reply: 1)
Difference between PCR/cloning DNA in plasmids - (reply: 2)
What is the expected pattern of restriction digestion of human genomic DNA? - (reply: 2)
DNA purity - (reply: 1)
Genomic DNA contamination in my RNA sample? - (reply: 8)
RNase should be added in which step for gDNA isolation using phenol chloroform m - after lysozyme / preteinase k treatment or after phenol chloroform? (reply: 9)
Dna after gel extraction, single or double stranded? - (reply: 1)
Whole blood RNA and DNA extraction help - Expression analysis and epigenetics (reply: 2)
DNase treatment after RNA extraction using RNeasy kit of qiagen - DNaes treatment after RNA extraction (reply: 2)
Stability of DNA at RT - (reply: 1)
ChIP- Different DNA size for Input DNA and ChIP DNA on Agilent Bioanalyzer - (reply: 2)
How to get rid of DNA/RNA in protein expression preps - Is there an alternative to DNAse? (reply: 1)
Smear and fluorescent in the well, could it be DNA or ? - (reply: 1)
no band observed for bacterial genomic DNA during agarose gel electrophoresis - (reply: 9)
Gentle method to dry a DNA pellet after alcohol precipitation - (reply: 5)
Extraction of 12 bp DNA from polyacrylamide gel - (reply: 2)
dna with very low GC content? - (reply: 2)
PCRing small region on genomic DNA - (reply: 3)
ChIP on chondrogenic pellets -- help! - no DNA signal at all (reply: 4)
dna contamination after turbo free dna - (reply: 2)
Laser failure problems with ABI 3730xl DNA Analyzer - (reply: 1)
Musca domestica gDNA isolation - (reply: 3)
Funny DNA bands on Agarose gels - (reply: 6)
Is DNAse I step necessary after RNA synthesis by in vitro transcription? - (reply: 1)
Remove gDNA from RNA sample - (reply: 3)
Why does freezing/thawing DNA samples first improve PCR results? - (reply: 8)
How does a DNA binding column work - (reply: 3)
smears in PCR products (bisfulite treated DNA) - (reply: 4)
DNA Quantification - Problems with DNA Quantification (reply: 2)
No DNA after PCR purification with QiaQuick? - (reply: 4)
dna extraction question - (reply: 2)
DNA extractio kit - (reply: 8)
genomic dna isolation - (reply: 5)
DNA denaturation and renaturation - (reply: 1)
DNA FISH protocol, wondering what some of these steps are for - (reply: 3)
DNA stored in Ethidium Bromide degrade easily? - From CsCl prep (reply: 1)
PCR genomic DNA using cell lysate - (reply: 1)
DNA purification from restriction digest - It keeps on degrading :( :( :( (reply: 4)
Amount of DNA to send for sequencing - linear response in signal intenstiy? - (reply: 1)
How is bisulfite converted DNA unstable over time? - (reply: 4)
Putative tumor suppressor promoter heavily methylated yet upregulated by DNA dam - (reply: 3)
Problem loading DNA / agarose gel - (reply: 14)
DNA quantification of BAC clones - (reply: 1)
Bacterial DNA Extraction - possible of contamination? - (reply: 7)
smear in pcr +DNA polymerase mixture for long PCR - (reply: 10)
1-day old culture for DNA extraction - plausible? - (reply: 2)
Using DNA as a reference in qRT-PCR - (reply: 1)
Doing PCR on Nebulized DNA - (reply: 2)
Centrifuge speed for DNA extraction - Help (reply: 4)
RNA carrier when using not so good DNA for bisulfite conversion - (reply: 3)
Genomic DNA purity...??? - strange 260/280 and 260/230 ratios (reply: 7)
Question about RNA extraction & gDNA elimination - can you really eliminate gDNA & how do you like to do it? (reply: 1)
Cleaning up old (not quite ancient) gDNA extractions - (reply: 5)
Help! How much DNA to use in IP reaction? - (reply: 1)
3 distinct bands of gDNA on gel - (reply: 2)
How rapid are DNA methylation changes? - (reply: 1)
DNA extraction from E. coli - solutions needed? (reply: 6)
Internal Control RNA or DNA - (reply: 4)
Weird bands in standard PCR of gDNA and cDNA - (reply: 2)
DNA pellet - Does not stick to the vial (reply: 18)
DNA detection limit of the PCR - Calculating detection limits (reply: 4)
Proportion of colonies containing DNA inserted at the cloning site - (reply: 1)
What causes RNA/DNA to unbind from silica membrane? - (reply: 1)
Can Qiagen columns remove genomic DNA? - Settling a debate. (reply: 6)
can contaminating gDNA explain this? - (reply: 1)
ClustalW question - on multiple alignment of DNA sequences (reply: 1)
My DNA pellet doesn't dissolve - Overdried?? (reply: 3)
DNA, Gel - Possible reasons for DNA Gel Smear (reply: 12)
DNA amount calculation for PCR - (reply: 14)
Is T4 DNA Polymerase so evil? Blunt End Cloning - (reply: 3)
Question: Start with genomic DNA or mRNA for methylation analysis - (reply: 4)
why cut plasmid DNA with Hind111 and EcoR1? - (reply: 1)
siRNA DNA co-transfection - (reply: 3)
Storing genomic DNA in 4C with PBS? - (reply: 2)
Problems PCR-labeling DNA probes with DIG-dUTP - (reply: 3)
Odd question. Can I use DNase to decontaminate Primers? - Primer decontamination (reply: 2)
uncut plasmid dna vs linearized plasmid gel - (reply: 1)
control DNA transformation- using pGEM kit - transformation (reply: 4)
PCR for sequencing genomic DNA (multiple alleles) - Are there preferences for amplification of certain sequences? (reply: 1)
PCR of complement genomic DNA - (reply: 3)
Taq DNA polymerase - how can microbes affoard an error-making enzyme? (reply: 2)
High Concentration DNA Extraction - how to get high conc without stickyness? (reply: 4)
PCR for long and repetitive region from genomic DNA - (reply: 4)
dna isolation - dna isolation (reply: 1)
How to store plasmid DNA on filter paper - I want to make a folder of all of them (reply: 3)
How do I PCR a DNA fragment with >200 CGG repeats? - (reply: 2)
DNA and histone methylation help - (reply: 1)
DNA extraction from plated colonies - (reply: 3)
Transcription factors that dont bind DNA - (reply: 8)
Sense and antisense DNA, and primer design - (reply: 6)
Forward primer not working during DNA sequencing - DNA sequencing (reply: 10)
DNA isolation - ethanol precipitation related (reply: 2)
plasmid DNA versus genomic DNA - concentrations (reply: 1)
Is DNase necessary for primers designed on the exon-exon boundary?? - (reply: 2)
mirna overexpression/ library versus single construct/ unspecific pcr - unspecific band when pcr on gDNA from library but not on gDNA fron sin (reply: 2)
Difficult to extract DNA from vulcanic coastal sediments - (reply: 2)
Which acetat for a DNA isolation - (reply: 3)
Is it possible to separate DNA with only 10bp difference? - (reply: 3)
mini-prep plasmid isolation by alkaline lysis - genomic DNA vs plasmid DNA (reply: 6)
Estimation of DNA Quantity on an Agarose Gel - (reply: 5)
isolating 40bp DNA for recombination - (reply: 1)
RT-PCR: DNA contamination, one vs two step - RNA work (reply: 11)
white residue after DNA purification - (reply: 3)
DNA probes for In Situ Hybridization - equivalence of Sense probe for control for Dig-DNA-label probe? (reply: 1)
Human DNA fingerprinting - (reply: 19)
Yeast Genomic DNA preparation - (reply: 4)
genomic DNA extraction: up to 50kb? - genomic DNA isolation (reply: 1)
About human DNA sequence identity - (reply: 6)
Isoamyl Alcohol in DNA extraction - (reply: 3)
DNA contamination of RNA samples for qPCR...weird results! - (reply: 3)
need to know minimum amount of template DNA needed for pcr amplification - pcr (reply: 2)
MaestroGen UltraSlim LED illuminator - for DNA gel (reply: 1)
What's the highest DNA concentration for PCR? - before being inhibited? (reply: 7)
Amplification of bisulfite converted gDNA for sequencing - (reply: 1)
PCR amplification with Pfu / quality of DNA - (reply: 4)
DNA extraction from paraffin embbed tissue - How can extract DNA form tissue (reply: 2)
Genomic DNA electrophoresis? - (reply: 1)
Saliva DNA extraction help - (reply: 3)
Chip-seq blocking reagent - Samlon DNA is useful for Chip-chip (reply: 1)
Chip-seq blocking reagent - Salmon DNA is useful for Chip-chip (reply: 1)
Effect of a DNA prep on DE3 - (reply: 2)
DNA Extraction from bacterial cultures - (reply: 3)
DNA from ticks - (reply: 3)
Using CDS DNA for expression - (reply: 3)
Extraction of bacterial DNA - (reply: 1)
DNA electrophoersis ladder very blurry - (reply: 4)
isolate DNA from amniotic fluid - (reply: 1)
DNA yields after Bisulfite Treatment - How can I optimize when I have low yield? (reply: 16)
gDNA had smear band - problem in extraction human genomic DNA (reply: 2)
Molecular Cloning od Recombinant DNA - (reply: 3)
RAPD analysis with not that pure DNA - (reply: 4)
Ethidium Bromide or safe dyes for DNA??? - (reply: 14)
DNA extraction from induced phage - (reply: 1)
DNA purification from blood - (reply: 6)
Annealing DNA - (reply: 4)
Cleaning contaminated DNA - separating human from bacterial DNA (reply: 1)
Large DNA extraction from an agarose gel - (reply: 8)
Dna cloning using NdeI/BamHI using pET11 vector - (reply: 4)
DNA isolation from plant nuclei by plasmid extraction kit - (reply: 1)
DNA isolation from human serum/plasma - (reply: 1)
Viral DNA extraction - (reply: 1)
gDNA restriction digest - (reply: 1)
remove carbohydrate from DNA preps - (reply: 4)
Adding Restriction Site to DNA - (reply: 3)
DNA ext from paraffin-embedded tissue ... a dilemma ! - FFPE tissue refuse to lyse strange whitish clumps formed!! (reply: 1)
genomic DNA extraction - why there appears a smear on the gel photo (reply: 11)
cDNA convertion to DNA - (reply: 4)
Cleaning RNase - How do i clean out the RNase from my genomic DNA (reply: 1)
mite DNA extraction - (reply: 7)
mite DNA extraction - (reply: 1)
DNA molecular weight keeps changes - (reply: 3)
SNP and DNA methylation - (reply: 1)
regenerate qiagen midi columns - how to reuse the midiprep DNA columns (reply: 3)
DNA stuck in gel well - some samples are stuck in the agarose-gel well and don't migrate (reply: 4)
Generating Standard for qPCR - PCR vs plasmid DNA (reply: 1)
salt for DNA precipitation - DNA purification (reply: 3)
Eliminate bacteria(l DNA) from hypochlorite-treated nematode eggs - (reply: 2)
DNA quantification after gel purification - (reply: 6)
DNA sequencing question help - make up sequencing mastermix (reply: 1)
G to A transition in bisulfite-treated DNA - (reply: 2)
Why isnt Phenol miscible in Tris Buffer? - Isolation of Genomic DNA (reply: 1)
Microtiter plate directed DNA degradation - Why is DNA on one plate degraded while intact on the next? (reply: 3)
Whole Genome Amplification of bisulfite treated DNA - (reply: 7)
DNA sample contaminated with lipids - (reply: 8)
Problem with gel extraction of DNA. - (reply: 1)
If a 20bp primer differs from template DNA by 20bp, can PCR work? - (reply: 1)
How to clone DNA (already cloned in a vector) in to another vector - please help (reply: 20)
genomic DNA isolation - (reply: 1)
DNA product almost 1500 bp - (reply: 1)
Slightly too high 260:280 and 260:230 ratios from gDNA extraction - Should I care? (reply: 2)
DNA Sequencing / Cloning ? - (reply: 5)
gDNA extraction with pestle and mortar without liquid nitrogen? - (reply: 5)
Help with DNA Quantification - Cell lysis for DNA quantification using Hoechst 33258 (reply: 2)
DNAse digestion while RNA extraction with TRIzol - anyone tried that before? (reply: 1)
Amount of DNase to use (Qiagen) - (reply: 7)
Restriction digestion of gDNA for phage library - (reply: 5)
DNA precipitation- Why EtOH? - Why we use EtOH and NaOAc together? (reply: 1)
How long can DNA precipitate at -20? - (reply: 6)
electrophoresis/ difference between expected size and result/ what's the alt - How to confirm the band means the expected DNA? Sequencing&what else? (reply: 5)
dna sequencing - (reply: 1)
dna fragments - (reply: 3)
internal control for genomic DNA - (reply: 1)
DNA pooling for PCR - Saving money PCR (reply: 7)
Sites for protein sequence from DNA sequence - (reply: 2)
DNA zap - (reply: 1)
problems when use qPCR to quantify bisulfite modified DNA - (reply: 4)
direct lysis of cell pellet - How to get rid of DNA? (reply: 3)
Basic DNA sequencing question - (reply: 5)
measurement units of DNA - (reply: 2)
measurement units of DNA - (reply: 4)
DNA Extraction - (reply: 1)
DNA bisulphited Amplification - the amplification dont produce any band!! (reply: 3)
PCR genomic DNA of high GC content - (reply: 6)
DNA extraction - (reply: 2)
DNA extraction form endoscopy samples - (reply: 4)
Functions of this reagents!!! - Please help me with DNA extraction!!! (reply: 1)
Can we find out own germline DNA - (reply: 1)
problem about DNA electrophoresis - the DNAs become two layers in the gel (reply: 4)
DNA molar mass - (reply: 1)
DNA loading buffer - (reply: 2)
Left DNA plasmid out for a week - (reply: 3)
PCR/DNA Extraction Problem - (reply: 5)
Extraction of DNA smaller than 100 bp from Agarose Gel - (reply: 6)
probably DNA is disturbing the quantification of my sample - (reply: 3)
PCR of AT-rich DNA - (reply: 1)
agarose gel of ligated DNA doesn't show any bands - (reply: 2)
How to view 75 bp DNA in gel - (reply: 4)
dna loading dye for proteins - (reply: 2)
DNA extraction - (reply: 1)
destroy DNA incorporated inside the pipettes - (reply: 5)
Isobutanol extraction of DNA - (reply: 2)
Looking for FASTA format of DNA of a gene amongst various species - (reply: 7)
Can I leave DNA in agarose gel piece overnight at 4oC before extraction? - Can I leave DNA in agarose gel piece overnight at 4oC before extractio (reply: 2)
DNA Precipitation - (reply: 2)
DNA damage in gel - (reply: 8)
Quantity of DNA - (reply: 3)
converting OD 600 reading to DNA concentration - Totally having a brain hiccup (reply: 3)
How much template DNA do you use for qPCR - (reply: 1)
DNA isolation from dark hair - (reply: 4)
Can a primer bind to DNA in a 5'-3 parallel fashion? - Just wondering... (reply: 1)
Stability of genomic DNA in 1x PBS - (reply: 2)
CLONING PROBLEM!!! - no DNA after ligation!!!!! (reply: 6)
Problems ligating shRNA primers - Sequencing reveals damaged DNA (reply: 1)
DNA degradation - (reply: 1)
DNase 1 treratment of RNA - (reply: 11)
NO DNA - Cant get DNA amount by spectrophotometry (reply: 1)
DNA extraction from very small volumes - (reply: 5)
dna extraction from soil samples - (reply: 4)
Measuring DNA, RNA, protein and cell wall synthesis - (reply: 1)
Plasmid DNA mini-prep - no good isolation (reply: 5)
ChIP-chip - how much DNA do you label? equal amounts? (reply: 1)
DNA fragmentation - (reply: 2)
qPCR of BS DNA - (reply: 6)
Real-Time PCR using genomic DNA (without DNA purification) - (reply: 3)
Best method for validating DNA methylation array data - (reply: 1)
Troubleshooting on DNA Extraction-Salt extraction - forgot to add SDS at the first step (reply: 1)
DNA contamination in RNA samples - (reply: 2)
DNA damage and repair - general questions about this subject (reply: 5)
Fungal DNA extraction from wheat - (reply: 5)
Glycogen - DNA precipitation? - (reply: 4)
bisulfite sequencing cloning problem (methylation of DNa) - bisulfite sequencing cloning problem (methylation of DNa) (reply: 3)
Precipitation in DNAse I incubation buffer - (reply: 1)
DNA purification - DNA purification dissolved in water (reply: 3)
purified dna using dh5alpha and qiaprep....plasmid not in genome - (reply: 3)
What is the minimum DNA concentration requried for double digestion? - (reply: 1)
Sample Grinding For DNA extraction - Sample Prep (reply: 3)
Streaky DNA digest - (reply: 3)
DNA extraction - why 100% and 70% ethanol? (reply: 13)
DNA agarose gel electrophoresis - sum1 can teach me interpret this gel image.. (reply: 3)
Help ADD 3 bases to DNA Sequences - (reply: 6)
Qiagen Qiaquick vs MinElute PCR purification kit for ChIP DNA - (reply: 10)
Freeze drying prior to DNA extraction - (reply: 1)
Genomic DNA - (reply: 2)
DNA sonication - RNA contamination? - New probe, start all over (reply: 13)
DNA quantification: NanoDrop vs. Picogreen - measurement of ChIP Input DNA (reply: 9)
Bad PCR, is it due to conditions, reagents, or lack of DNA template? - (reply: 1)
DNA extraction from mouse tail - (reply: 2)
DNA size markers run at different levels in the same gel - Same marker loaded in two lanes runs differently! (reply: 2)
Is DNA an acid because of phosphate group - (reply: 2)
partial DNA digestion formula - Linear DNA partial digestion (reply: 1)
DNA and salt contamination using mirVANa miRNA isolation kit - (reply: 6)
DNA Extraction - (reply: 2)
Isolation of DNA from Scat - (reply: 2)
genomic DNA isolation - (reply: 4)
Equalizing RNA samples for Reverse Transcription - Should I do this before or after DNA digestion (reply: 2)
Zymo EZ Methylation Gold DNA bisulphite treatment - No DNA present after treatment (reply: 5)
DNA Extraction - maize ssr (reply: 3)
cleaning sonicator tip to prevent DNA contaminationin samples - (reply: 1)
why they use isopropanal for precipitate DNA in midiprep - (reply: 5)
HELP with cell lysis for DNA assay - Something is floating in my lysate solution!!! (reply: 1)
Amplification from plasmid DNA, but not from genomic DNA with the same target re - (reply: 4)
DNA/RNA extraction from sputum - (reply: 3)
DNA extration from Bacteria spores - (reply: 13)
dam methylated DNA in dam - straights - (reply: 1)
Which Plasmid DNA for standard curve? - (reply: 5)
DNA stains - pronunciation question (reply: 3)
dna smear in electrophoresis - (reply: 6)
does anyone have experience with pwo DNA polymerase for long PCR fragments ? - (reply: 2)
HELP! Genomic DNA, RNA-size bands - (reply: 2)
Cell Lysis for DNA extraction - Not every sample is successful (reply: 5)
help needed: PCR a gene from genomic DNA - (reply: 2)
Dual cross-linking thoughts - Agents to help analyse proteins indirectly bound to DNA (reply: 16)
dna microarray - questions - (reply: 1)
some Q's about dna microarray - (reply: 2)
Genomic DNA Library - Problem of reactivity - Genomic DNA library screening with antibody (reply: 1)
Storage of gel extracted DNA before ligation - (reply: 3)
NICKED DNA - (reply: 2)
dna storage buffer - (reply: 3)
Amplification of human genomic DNA - (reply: 2)
trouble amplifying 2.5kb product from genomic DNA - (reply: 3)
Fungal DNA Isolation - Fungal DNA isolation without liquid nitrogen (reply: 1)
Can I use genomic DNA to make a standard curve for qPCR - (reply: 5)
very low ChIP'd DNA amount. - (reply: 1)
Comparing cell # or DNA content - of pelleted cells (reply: 1)
Help for some general DNA Extraction Questions - (reply: 5)
how to check the quality of DNA? - (reply: 4)
mtDNA - included in isolated gDNA? - (reply: 4)
DNA precipitation - (reply: 3)
plasmid DNA disappear in the minipre - (reply: 2)
Purify ChIP DNA using Qiagen spin filter - (reply: 8)
DNA contamination after RNA extraction, which kit works best? - (reply: 6)
Binding and eluting a DNA binding column - (reply: 1)
genomic dna isolated from blood is brown color?! - (reply: 4)
Removal of RNA from DNA sample - (reply: 15)
3-colour labeling for the single argon-ion laser? - DNA + 2 antibodies (reply: 1)
Only DNA ladder , No desired band in PCR - (reply: 4)
low total/input DNA levels in ChIP work-up - (reply: 7)
plasmid dna isolation - (reply: 1)
DNA and RNA contamination in RT PCR water controls - (reply: 2)
Long PCR and genomic DNA isolation problems - (reply: 2)
DNA supercoiling on agarose gel -> reference needed - (reply: 1)
DNA methylation analysis - direct sequencing gives CT peaks - (reply: 1)
DNA extraction from FFPE? - (reply: 2)
MYCOPLASMALOGY - MYCOPLASMA DNA EXTRACTION (reply: 7)
T4 DNA polymerisation - elongation or removal? (reply: 3)
Whole Genome Methylation Analysis Feasibility - Methylation analysis on DNA from rare cell populations (reply: 3)
DNA Ladder Issues - DNA Ladder Agarose Gel (reply: 5)
compound that block DNA replication in human cell - (reply: 1)
Isolation of genomic DNA from microbial consortia - (reply: 2)
EXtraction of DNA bands from acrylamide gel - (reply: 4)
do small pieces of DNA form a helix - (reply: 2)
Large Circular DNA - (reply: 1)
best method for DNA extraction - (reply: 3)
heredity of DNA methylation - (reply: 2)
purity of genomic DNA, low 260/230? - (reply: 1)
long DNA amplification - (reply: 11)
DNA Laddering - (reply: 1)
gDNA pcr product as standard for absolute quantification? - (reply: 1)
purification of a large amount of DNA - (reply: 3)
DNA ligation - (reply: 2)
global DNA methylation quiagen kit - (reply: 14)
DNA binding/EMSA question - question about controls in DNA binding/EMSA (reply: 4)
Concentration of DNA for Transformation - (reply: 1)
Request Genomic DNA of Burkholderia mallei - possible: collaboration or exchange DNA with pseudomallei culture (reply: 8)
Attaching a double stranded DNA to a surface - (reply: 1)
Another band after plasmid purificaiton - ran the plasmid DNA on a gel (reply: 2)
DNA Question (T/F) - (reply: 4)
what primers should I use for DNA sequencing? please help - (reply: 2)
what primers should I use for DNA sequencing?? - (reply: 3)
DNase and PMA/Iono - (reply: 1)
plasmid DNA lab question - (reply: 11)
Rescue my DNA extraction - (reply: 1)
denaturation of dna - (reply: 1)
PCR using genomic DNA - (reply: 2)
Using analytical grade reagents for DNA extraction - (reply: 6)
SOS....Genomic DNA extraction - Extraction from gram positive bacillus stearothermophilus (reply: 8)
Optimal stock concentration for gDNA storage - (reply: 1)
Manual reverse primer design for bisulfite traeted DNA - (reply: 4)
Using DNA in gel extract kept at -20ºC - (reply: 2)
Help! Plasmid disappearing after mini-prep. - A260 shows good quality DNA, but nothing appears on gel. (reply: 3)
Help with Phenol-Chloroform DNA Extraction - (reply: 3)
Further DNA purification steps needed - Post DNAeasy Kit Purification (reply: 4)
DNA yield estimation - (reply: 1)
Testing primer specificity... - ...without gDNA of target organisms (reply: 6)
Size of fusarium DNA - (reply: 1)
size of fungal DNA - (reply: 3)
Best way to desalt very salty, low yield DNA - (reply: 3)
Source of DNA for a Reporter Construct - How to PCR the promoter region of interest? (reply: 1)
weak ChIP signal - suggestions to increase the amount of DNA? (reply: 10)
DNA methylated PCR - (reply: 2)
Why can't genomic DNA be stored at 2-8 for long periods? - (reply: 10)
Genomic DNA from Blood Kits - (reply: 2)
Direct PCR (PCR without DNA extraction) - (reply: 2)
DNA concentration and Genome - (reply: 1)
DNA ladders on a northern blot - (reply: 1)
Accidently heated up the wrong DNA. Damage? - (reply: 2)
genomic DNA quality for genome walking - (reply: 2)
Large Molecular weight DNA Extarction - (reply: 7)
weak bands for methylated DNA - (reply: 4)
Amplfication of fungal DNA - (reply: 8)
No amplification in PCR using gDNA extracted from formailin fixed paraffin embed - (reply: 3)
dna quantification and quality assesement - abs 260nm / abs 280 nm <0.5 (reply: 2)
optimal template dna concentrations - (reply: 1)
Volume of DNA to add to PCR Master Mix? - (reply: 1)
how much DNA you need for real time pcr after chip - CHIP (reply: 1)
RNA Purification - After DNase (reply: 3)
How To Fix NaOH/Tris DNA Digest Error? - (reply: 1)
DNA extraction with expired kit? - (reply: 1)
protein/DNA Sequence Alignment - (reply: 1)
PCR with genomic dna - (reply: 9)
DNA Enrichment for Methylation Analysis - (reply: 8)
Finding corresponding amino acid in DNA sequence - (reply: 1)
Buffer for 5 Kb DNA fragments - (reply: 2)
Random library using TOPO vector - random genomic DNA ligated into TOPO, false inserts? (reply: 3)
Plasmid DNA sequence and map - PLASMID DNA (reply: 2)
DNA isolation - parasite - Cryptosporidium DNA isolation (reply: 3)
pcr doesnt show any band for DNa walking - (reply: 8)
DNA polymerases for PCR-sequencing - (reply: 11)
help-how to extract fluo-labeled DNA from agarose gel - (reply: 1)
Integrity of treated DNA - Is quality of bisulfite treated DNA poor? (reply: 1)
Qiagen DNAeasy blood&tissue DNA isolation kit - (reply: 1)
5-AZA, Reversing DNA methylation (epigenetic reactivation experiments) - (reply: 3)
Best method to send DNA? - (reply: 4)
protein precipitation with TCA for dna extraction? - (reply: 1)
no DNA after IP - (reply: 1)
Trouble with PCR on genomic tomato DNA (I've tried many fixes) - (reply: 5)
Best way to extract plasmid from Mycobacteria - Extraction of DNA (reply: 7)
purefication and amplification of serum free DNA - (reply: 1)
dnase treatment before his-tag purification - (reply: 3)
DNA extraction fron HSV-1 infected Vero cell - (reply: 2)
Freezing DNA have effects on real-time PCR efficiencies - (reply: 1)
low DNA yield after extraction - (reply: 1)
protein precipitation without phenol/chloroform for dna extraction? - (reply: 1)
Is gDNA digestion before bisulfite conversion important? - (reply: 5)
Agarose: DNA/dye mixture doesn't stay in the gel? - Thanks for any and all help received! =] (reply: 12)
DNA isolation from tissue !!SOS!! - (reply: 1)
GC rich DNA sequencing - (reply: 3)
Gemonic Library - Deletion/Reduction of Chloro/Mit DNA (reply: 1)
Plasmid DNA isolation - neutralisation of DNA (reply: 5)
transcription - quality of DNA (reply: 1)
Sterilize DNA preps - (reply: 5)
Transcription - Quality of DNA (reply: 1)
DIG DNA PCR labeling problem - (reply: 6)
Storage/Shipping of DNA at ambient temps - (reply: 2)
how to get rid of DNA (in TE) contamination - protein and phenol removal (no kits please) (reply: 17)
Trizol RNA/DNA extraction - (reply: 3)
dna not running for electrophoresis - (reply: 9)
nondenaturing PAGE to separate dna - (reply: 4)
RNA isolation for real-time RT-PCR - forgot to inactivate the DNAse (reply: 4)
failed PCR on DNA extract from blood - (reply: 6)
Problem encountered when dosing my gDNA by spectrophotometry - Never experienced such thing before! (reply: 7)
DNA methylation prediction in plant DNA - (reply: 2)
Phenol - why different for DNA and RNA extraction? (reply: 9)
concentration of dna - (reply: 3)
DNA dissolved in DMSO instead of H2O - (reply: 2)
PCR contamination with human DNA first and now no bands!!! - (reply: 4)
Questions regarding bisulfite treatment of DNA - (reply: 2)
Fragmented DNA doesn't appear as bands on agarose gel - (reply: 5)
Questions about 260/230 when reading DNA conc. - (reply: 1)
Genomic DNA contamination of RNA - (reply: 24)
dna separation in native PAGE - (reply: 3)
DNA extraction and PCR advice requested - PCR, Contamination, Low abundance targets (reply: 7)
Problem with DNA extraction from 1% agarose Gel - No DNA using Qiagen Qiaek 2 and Spin column kits (reply: 9)
How stable is methylated DNA? - (reply: 4)
dna concentration - please help (reply: 5)
volume of DNA required (PCr product ) in agarose gel - (reply: 11)
Restriction endonuclease - cut only double stand DNA? (reply: 4)
separation of dna fragments in agarose gel electrophoresis - (reply: 7)
Primer Design - Design Primer with DNA Sequence (reply: 1)
Diluting DNA - (reply: 1)
Dry Plant leaf samples for DNA extraction - (reply: 1)
DNase I pre-treatment - how can i be sure that it was efficient??? (reply: 2)
PCR without DNA extraction! - (reply: 2)
dna degradation - (reply: 4)
Gel shift with large DNA fragments (>1 kb) - Can I still do EMSA? (reply: 1)
Filtration DNA before transfection? - (reply: 4)
Genomic DNA and PCR - (reply: 4)
DNAse activity in room temperature - (reply: 3)
Can DNA be solubilized in hydrochloric acid? - (reply: 2)
about the total RNA isolation (trizol methods) and further Dnase I - (reply: 3)
Presence / lack of introns in genomic DNA of related species - (reply: 2)
DNA cloning - (reply: 4)
freezing of E.coli culture prior DNA prep - (reply: 5)
EZ DNA Methylation kit - (reply: 1)
DNA storage @ room temp on filter paper - (reply: 2)
DNase I digestion - how long the fragments are at the end??? (reply: 3)
Vortexing genomic DNA, detrimental or not? - (reply: 8)
Transfection efficiency vs. amount of plasmid DNA - (reply: 1)
DNA amplification but no bands on agarose gel, GC rich product, Roche - DNA amplification but no bands/smear in agarose gel (reply: 5)
Genomic DNA copy number qPCR - Help with control (reply: 8)
Why were proteins favoured over DNA as the genetic material before 1940? - University Genetics Past EXAM Question (reply: 2)
Sources of Contamination in plasmid DNA Mini-Preps - (reply: 10)
Genomic DNA contamination in RNA sample - (reply: 2)
Isolation DNA and protein from a single sample - (reply: 5)
DNA extraction from saliva/buccal swabs - (reply: 5)
DNase I digestion - check if it worked?? (reply: 4)
elution of dna from gel - (reply: 5)
Question about DNA electrophoresis - (reply: 1)
EZ DNA methyaltion kit...trouble shooting - (reply: 4)
Could You Suggest me A good KIt for DNA extraction from Yeast? - (reply: 1)
DNA extraction trouble - (reply: 7)
DNA vs Protein Stains - Are there any that don't stain BOTH? (reply: 1)
Extraction of large size genomic DNA - (reply: 3)
Colored DNA after phenol extraction - Strange color in aqueous phase and degraded DNA... (reply: 1)
CHIP - DNA concentration after sonication - (reply: 6)
DNA Question for TV Show - (reply: 4)
Principles behind DNA RNA protein isolation and purification - (reply: 2)
Phenol chloroform extraction of DNA - (reply: 3)
Shrimp nuclease to eliminate genomic DNA - (reply: 7)
gDNA or cDNA amplification in RT-PCR - (reply: 3)
suggestions on the genomic DNA quality - (reply: 2)
ChIP DNA quality - problem with ChIP-qPCR (reply: 5)
Dnase digestion and PCR - (reply: 4)
Why is it necessary to add Taq DNA polymerase last during PCR? - (reply: 8)
Promoter and genomic DNA - (reply: 2)
Genomic DNA - (reply: 9)
is there DNA ? - how to know ? (reply: 8)
DNA digestion - Restriction Enzymes (reply: 3)
Problem with DNA purification from agarose gel - (reply: 3)
T4 DNA Ligase & Restriction Enzyme Expression - I am looking to save money! (reply: 4)
DNA extration of pieces of human hearts - (reply: 2)
DNA concentration from ultraspectrophotometer is not accurate - (reply: 6)
RNA appearance on 1.5% gel after DNase I digestion - (reply: 2)
DNA precipitation - (reply: 5)
DNA folding to form the loop - (reply: 2)
DNA purification - long fragments wanted - (reply: 1)
Unidentified DNA bands - (reply: 1)
Any good way to remove DNase? - (reply: 5)
DNA shearing with Misonix 4000 and microplates cup horn - (reply: 2)
Accidentally digested DNA overnight - still OK? - (reply: 4)
dna for real time - (reply: 3)
Remove RNA after DNA mini-prep - (reply: 1)
Confirm DNA enrichment of methylation array - (reply: 2)
Capillary DNA Sequencing - (reply: 6)
can we use one-step RT-PCR kits to amplify DNA? - (reply: 1)
Why is Cleavage of DNA using restrcition enzyme more suitable for genetic engine - !! confused confused confused !! (reply: 4)
How pure is my DNA sample?? - Confused-need help urgently please! (reply: 1)
unmethylaten DNA control - ?????????? (reply: 3)
Tips for genomic DNA extraction from gram -ve bacteria - (reply: 2)
M.SssI treatment of DNA - (reply: 1)
Gel purification of vector DNA - low yield - (reply: 26)
DNA IDENTIFICATION - HOW TO TELL THE DIFFERENCE (reply: 14)
DNA extraction methods - PCI extrction (reply: 3)
Plant DNA extraction - (reply: 5)
Seperate DNA spin columns - (reply: 3)
DNA quantification - (reply: 5)
Digest genomic (eukaryote) DNA before running PCR, is it necessary? - I do not get any band from PCR using genomic DNA and different primers (reply: 6)
Problem with DIG High Prime DNA Labeling and Detection Starter Kit II - (reply: 2)
PCR from a smear genomic DNA ? - (reply: 5)
DNA/RNA into nucleotides - (reply: 6)
Fast, cheap and dummy proof large scale DNA extraction? - (reply: 6)
difference between a linear and circular plasmid/DNA for stable transfections - will a circular plasmid stably integrate into the genome? (reply: 4)
Help with Purifying DNA Hairpin - (reply: 2)
Reuse DNA spin column - (reply: 22)
Does UV cause DNA fragmentation? - how long do I need to put cells under UV to see fragmentation? (reply: 12)
Best storage temperature for samples - serum, protein, tissue, DNA, RNA - Archives (reply: 7)
Does phenol extraction have effect on DNA methylation? - (reply: 4)
Selection during Extraction: Plasmid or Genomic DNA? - (reply: 1)
RNase free syringe for Qiagen DNase treatment, could not find any company provid - (reply: 2)
gDNA purification - anything better than alcohol precipitation? (reply: 13)
Effect of freezing on DNA methylation? - (reply: 6)
DNA sequencing analysis - DNA sequencing analysis (reply: 1)
DNA sequencing analysis - DNA sequencing analysis (reply: 11)
qPCR quantification of ChIP DNA - (reply: 2)
In vivo transfection of plasmid DNA, how? - intramuscular injection DNA (reply: 2)