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DNA Related Discussions
  1. How to select promoter for resistance gene in linear/naked DNA "knockout" - (reply: 2)
  2. Low RNA yield in dual RNA/DNA extraction - (reply: 1)
  3. dna isolation - (reply: 1)
  4. DNA isolation protocol needed!!!! - (reply: 1)
  5. dna isolation from bacterial cultures - (reply: 4)
  6. Visualization of DNA:RNA hybrid on TBE-UREA GEL - (reply: 8)
  7. Canīt get rid of DNA contamination in RNA - (reply: 2)
  8. PCR DNA bands too large on agarose gel - (reply: 1)
  9. How can DNA purity be checked? (original title "PROTOCOLS") - (reply: 1)
  10. Bacteria grow on antibiotic selective plate but plasmid extracted showed no dna - (reply: 6)
  11. Thermo scientific T4 DNA ligase product help - (reply: 3)
  12. RNA extraction and DNase treatment - (reply: 1)
  13. RNA extraction and DNase treatment - (reply: 2)
  14. Second generation DNA synthesis column - (reply: 1)
  15. concentrate DNA - (reply: 3)
  16. DNA SDS-PAGE electrophoresis - (reply: 6)
  17. Low plasmid yield after Miniprep, DNA in the first wash flow through (PB) - (reply: 1)
  18. All I get is supercoiled DNA... - (reply: 5)
  19. DNA shows on Nanodrop but not on electrophoresis - (reply: 2)
  20. Simple DNA analysis software for PC? - (reply: 2)
  21. MNase assay - how to quantify DNA - (reply: 3)
  22. Genomic DNA quantification using the NanoDrop - (reply: 3)
  23. Determine DNA sequence expression in cell line - (reply: 1)
  24. Separaing double stranded dna - (reply: 1)
  25. Methods to Sequencing "Unknown" DNA - (reply: 2)
  26. Low 260/230 ratios from DNA purification question - (reply: 1)
  27. EZ DNA Methylation-Lightning Kit Incomplete Conversion‏ - (reply: 5)
  28. Quantuitative staining of RNA and DNA? - (reply: 4)
  29. DNA loading dye contents - (reply: 3)
  30. ChIP: Inconsistent reverse crosslinking and DNA purification result - (reply: 2)
  31. DNA disappearing after restriction - (reply: 10)
  32. Decontaminating DNA - Effective Means? - (reply: 4)
  33. first time running DNA polyacrylamide TBE gel - (reply: 4)
  34. DNA gel elctrophoresis problem - (reply: 3)
  35. DNA extraction problems. - (reply: 3)
  36. No sequencing of DNA methylation - (reply: 6)
  37. Transforming dna from agarose gel - (reply: 3)
  38. question on calculation DNA amount - (reply: 5)
  39. DNA exraction from parasites - (reply: 1)
  40. Why would you use DNase as a control in dot blot assay confirming viroid import? - (reply: 1)
  41. ethanol precipitation plasmid DNA - (reply: 3)
  42. Missing DNA Target and Effect on Non-Specific Amplicons - (reply: 1)
  43. DNA extraction from dissociated embryos - (reply: 2)
  44. DNA stability in STE with proteinase K - (reply: 1)
  45. Cell lysis and DNA quantification - (reply: 1)
  46. What is the protocol to remove RNA contamination (Using RNase) from DNA? - (reply: 8)
  47. Bacterial DNA extraction/purification from animal tissue - (reply: 1)
  48. PCR of bisulfite converted DNA is now producing a smear? Previously produced a s - (reply: 2)
  49. DNA damage or genomic instability: How to evaluate them? - (reply: 1)
  50. chk effect of cell line genes on normal dna - (reply: 1)
  51. Nanodrop machine error message when measuring plasmid DNA concentration - (reply: 3)
  52. DNA extraction from Vermiculite - (reply: 8)
  53. Can you store DNA in loading dye buffer short term? - (reply: 2)
  54. DNA Global Methylation with Epigentek kit-having trouble - (reply: 1)
  55. Quick method for DNA extraction using lysis - (reply: 4)
  56. DNA virus purification from agarose failed! - (reply: 7)
  57. Any real difference between DNA purification kits? - (reply: 9)
  58. DNA sequencing for GC rich sequence - (reply: 3)
  59. Subsequent DNA and siRNA transfection - (reply: 9)
  60. DNA cleanup after linearization, before transformation? - (reply: 3)
  61. A modified method for blunt end DNA cloning!!!!!!! - (reply: 2)
  62. Which method for DNA purification b4 cloning - (reply: 4)
  63. gDNA still presents even after DNase treatment - (reply: 6)
  64. DNA Extraction from buccal swabs - (reply: 7)
  65. Dna isolation from fungi - (reply: 2)
  66. PCR and sequencing of genomic DNA - (reply: 4)
  67. Purified DNA fragments are bigger than unpurified - (reply: 2)
  68. Genomic DNA Isolation from Soft Agar Colonies - (reply: 3)
  69. DNA extraction from endive (low yield + peak at 220 nm) - (reply: 4)
  70. Low RNA quantity after on column DNAse digestion - (reply: 1)
  71. enviromental water samples DNA isolation - (reply: 2)
  72. Can I concentrate already extracted DNA with an extraction kit!? - (reply: 9)
  73. PCR inhibitor in template DNA - (reply: 3)
  74. PCR from protozoa DNA - (reply: 3)
  75. Midiprep from mini prep DNA - (reply: 2)
  76. Alternative phenol-free method for DNA extraction - (reply: 2)
  77. Live cell sort DNA stain permeabilization - (reply: 1)
  78. DNA Quantification of PCR Products - (reply: 2)
  79. Plasmids DNA on filter paper - (reply: 1)
  80. How to qualitatively measure DNA methylation of gene promoter - (reply: 1)
  81. Loss of DNA after restriction digestion cleanup - (reply: 2)
  82. Testing for DNA degradation prior to Southern Blot Experiment - (reply: 2)
  83. Weird fragments in my gDNA - (reply: 2)
  84. HELP - Adaptor ligation to DNA not working! - (reply: 1)
  85. Dissolving DNA Oligos - (reply: 2)
  86. Using 10,000 rpm instead of 14,000 for DNA extraction with Promega kit - (reply: 2)
  87. DNA from frozed cells??? - (reply: 2)
  88. DNA Gel purification column issues/mistakes? What's your process? - (reply: 6)
  89. does DNA methylation outside promoter region affect gene Transcription/expressio - (reply: 1)
  90. faint dna band after pcr purification - (reply: 1)
  91. Storage life of plasmid DNA in water - (reply: 5)
  92. Weirdest thing with DNA dye and agarose gel - (reply: 4)
  93. How to design primer to amplify genomic DNA? - (reply: 3)
  94. How many fibroblasts for 1ug DNA? - (reply: 2)
  95. Plasmid DNA digestion problem - (reply: 3)
  96. PCR products sizes and DNA ladder - (reply: 7)
  97. No Band With Large DNA (BAC) - (reply: 1)
  98. DNA Liquid into DNA pellet - (reply: 4)
  99. DNA isolation from whole blood + clotting??!! - (reply: 2)
  100. need help (DNA) - (reply: 2)
  101. free online DNA & plasmid editing tool - (reply: 1)
  102. Plant DNA analysis by UV spectrophotometer indicates there is DNA in my sample, - (reply: 1)
  103. Manipulation of target DNA fragments - (reply: 1)
  104. Smeared DNA ladder - (reply: 3)
  105. DNA extraction problem - strange bands - (reply: 4)
  106. Nanodrop vs. picogreen: 100fold difference DNA yield from buccal swab!? - (reply: 9)
  107. atom replacement in DNA - (reply: 1)
  108. Loading dye in DNA - (reply: 2)
  109. DNA extraction impurities - (reply: 23)
  110. phenol/chloroform extraction of genomic DNA - (reply: 6)
  111. Phenol-Chloroform Extraction (DNA) - (reply: 5)
  112. genomic DNA extraction on silica-based membrane - (reply: 1)
  113. DNA extraction from the gel - low yield - (reply: 2)
  114. Help!! Double band in genomic dna - (reply: 1)
  115. DNA extraction (Phenol chloroform Vs NaOH) - (reply: 5)
  116. Reconstituting plasmid DNA from blotting paper - water vs. TE buffer - (reply: 6)
  117. DNA extraction , phenol , ice . plz help ! - (reply: 3)
  118. Dotted bands on my DNA agarose electrophoresis gel. - (reply: 2)
  119. I dissolved the DNA in 1XTAE instead of TE buffer, will it affect ligation? - (reply: 1)
  120. Frameshift mutation in DNA sequencing? - (reply: 1)
  121. identification of transcription factors binding to DNA - (reply: 1)
  122. Gel purification (DNA) - (reply: 1)
  123. DNA contamination from adenovirus afted DNase treatment - (reply: 2)
  124. Labeling DNA with Biotin - (reply: 2)
  125. DNA extraction kits - (reply: 3)
  126. Sigma Imprint DNA methylation quantification assay kit - (reply: 3)
  127. How to determine the location of integrated DNA in stable cell lines - (reply: 2)
  128. Viral contamination in DNA purification columns - (reply: 1)
  129. Information about the use of DNA diluted in Real Time PCR - (reply: 6)
  130. Running Plasmid DNA on Agarose Gel (Banding Pattern) - (reply: 1)
  131. PCR failing for transformed plant DNA but not for Agro - (reply: 3)
  132. problems with gDNA doing real time PCR in yeast - (reply: 2)
  133. Question about DNA purification from a restriction digestion reaction - (reply: 1)
  134. Simultaneous RNA and DNA transfection? - (reply: 2)
  135. standard curve using genomic dna - (reply: 1)
  136. DNA storage degradation - (reply: 1)
  137. Qiagen midi prep DNA yield - (reply: 3)
  138. Sodium Acetate pH 5.2 or 5.5 for DNA precipitation - (reply: 1)
  139. Column for removing short DNA digestion products - (reply: 6)
  140. What is the membrane made of in DNA purification kits - (reply: 3)
  141. Removing Introns from a DNA sequence - (reply: 1)
  142. DNA extraction from a partially-eaten fruit - (reply: 5)
  143. Quantifying bisulfite-converted DNA (pre- and post-pcr) and direct sequencing - (reply: 2)
  144. How to get rid of DNA contamination in RNA prep - (reply: 4)
  145. DNA sequencing - (reply: 2)
  146. DNA ladder does not resolve well - (reply: 4)
  147. Qubit quantification too low yield DNA - (reply: 4)
  148. AcONa 3M switched by AcOK 3M during DNA precipitation! - (reply: 1)
  149. Will long exposures of DNA to UV (302nm) alter sequencing data? - (reply: 4)
  150. types of dna cloning methods - (reply: 2)
  151. dna left on thermocycler - (reply: 2)
  152. Northern blot DNA probes - (reply: 1)
  153. How to avoid RNA contamination in DNA samples:? - (reply: 1)
  154. RNA contamination in DNA samples - (reply: 2)
  155. Global DNA hypomethylation assay - (reply: 1)
  156. removing foreign DNA - (reply: 4)
  157. Limit of detection for salmon gDNA? - (reply: 7)
  158. Doubt: genomic dna library construction - (reply: 3)
  159. Drugs that cause DNA double strand breaks - (reply: 1)
  160. SBI Minicircle DNA Kit -- No colonies -- ligation / transformation problem - (reply: 8)
  161. No DNA after gel extraction - (reply: 9)
  162. DNA isolation from nuclei - (reply: 5)
  163. DNA in 1water:0,1TE at 37 degrees - (reply: 1)
  164. Plasmid DNA contamination - (reply: 5)
  165. Problems with plasmid DNA extraction - (reply: 3)
  166. percentage DNA fragmentation - (reply: 1)
  167. DNA laddering - (reply: 1)
  168. Protocol for DNA fragmentation Analysis - (reply: 2)
  169. nicked linear DNA - (reply: 5)
  170. DNA isolation - (reply: 4)
  171. Plasmid DNA - (reply: 3)
  172. Scaling up PCR to get more DNA - (reply: 5)
  173. Cleaning DNA - (reply: 2)
  174. Buying a DNA synthesizer- advice, tips? - (reply: 1)
  175. Genomic DNA and plasmid DNA isolation - (reply: 3)
  176. Extraction of DNA from termite gut flagellates - (reply: 7)
  177. autoclaving tubes for DNA extraction - (reply: 2)
  178. Genomic DNA extraction from filters - (reply: 3)
  179. accidentally used denatured ethanol for DNA and RNA - what to do? - (reply: 4)
  180. Phenol Chloroform Isoamyl Alcohol (25:24:21) DNA purification - (reply: 3)
  181. overlap in the DNA sequence - (reply: 6)
  182. Low DNA concentration for sequencing - (reply: 5)
  183. No DNA after PCR product purification - (reply: 9)
  184. DNA extraction from whole blood - (reply: 6)
  185. DNA extraction using qiagen kit - (reply: 4)
  186. Role of sodium acetate in DNA extraction/precipitation - (reply: 7)
  187. using sybergreen to quantify DNA and assess its quality - (reply: 1)
  188. mtDNA to nDNA Ratio gDNA - qPCR - (reply: 1)
  189. High Molecular Weight DNA extraction and PFGE - (reply: 2)
  190. the cultivation of genetics and DNA of Kazakh people - (reply: 3)
  191. non redundant DNA - (reply: 4)
  192. Grass genomic DNA extraction - (reply: 1)
  193. Template DNA for PCR- Concrentration or Volume?? - (reply: 1)
  194. cell free DNA and maintaining stability - (reply: 3)
  195. Sensitive, Consistent and Reproducible Method(s) for Extraction of gDNA from Low - (reply: 2)
  196. PCR DNA Concentration - (reply: 1)
  197. Quantify total DNA in small cell lysate...? - (reply: 1)
  198. Quantify DNA in gel electrophoresis bands - (reply: 7)
  199. What happen to my animal DNA extraction? - (reply: 12)
  200. Homozygote DNA but Heterozygote RNA? - (reply: 2)
  201. Slime formation in DNA extraction - (reply: 12)
  202. Help: Best way to disrupt blood cells to generate blood debris and gDNA to be us - (reply: 4)
  203. How double stranded DNA becomes single stranded in LAMP assay? - (reply: 3)
  204. DNA Methylation and genomic variation - (reply: 2)
  205. Trouble with DNA purity (A260/A280) - (reply: 3)
  206. Straight bands on DNA gel - (reply: 3)
  207. Whole Genomic DNA and when to cut - (reply: 2)
  208. DNA size band increased after gel electrophoresis with Quaigen fit - (reply: 4)
  209. Recipe for TKM1 and TKM2 buffer for DNA isolation from Whole blood - (reply: 1)
  210. qPCR with Genomic DNA - (reply: 1)
  211. DNA extraction from whole blood using phenol chloroform - (reply: 2)
  212. No bands in PCR after DNase treatment - (reply: 4)
  213. Can anyone vouch for a protocol to get good gDNA from fungi? - (reply: 2)
  214. Successful Transformation->Getting Colongy--> Getting DNA after miniprep - (reply: 9)
  215. Which DNA strand is recommended - (reply: 1)
  216. Qiagen column eluted DNA gives higher concetration than the original sample - (reply: 10)
  217. System to label DNA - (reply: 6)
  218. Why do only some nuclear lysis buffers create DNA 'goop' - (reply: 1)
  219. DNA ligation and trasformation - (reply: 6)
  220. AA/DNA Sequence Splitter and PDB to FASTA converter - (reply: 2)
  221. Quality of Bisulfite Converted DNA - (reply: 2)
  222. how to find out the DNA seuence of a human gene from online database? - (reply: 1)
  223. too much DNA on agarose gel - (reply: 9)
  224. PCR not working for 5 Aza treated DNA - (reply: 3)
  225. Fast, cheap and dependable DNA extraction protocol for students? - (reply: 3)
  226. isolating DNA from cells in suspension - (reply: 5)
  227. DNA polymerase for GC rich template? - (reply: 5)
  228. Replication of the Plasmid DNA - (reply: 6)
  229. DNA extraction - PCR Problem - (reply: 3)
  230. different PARTS different DNA ,,, - (reply: 4)
  231. DNA extraction from non-viable PBMCs - (reply: 2)
  232. Threw ribosome or DNA - (reply: 4)
  233. Choose Ribosome or DNA - (reply: 3)
  234. Storing Many DNA Samples - (reply: 2)
  235. Question about isolating DNA from the blood sample - (reply: 2)
  236. how can I remove the pigments during DNA extraction? - (reply: 1)
  237. how to remove brown pigment in Sargassum DNA isolation? - (reply: 4)
  238. Is there any software that can predict DNA binding proteins based on input DNA s - (reply: 2)
  239. isolating DNA from mouse tails and subsequent problems with pcr - (reply: 7)
  240. Restriction enzyme - PCR sheep blood gDNA - (reply: 5)
  241. Viral genomic DNA isolation ? - (reply: 3)
  242. Porportion methylation of bisulfite DNA changes with time? - (reply: 4)
  243. 615 or 660? Average MW of a DNA base pair - (reply: 3)
  244. DNA EtOH/Sodium Acetate precip over the weekend...still good? - (reply: 2)
  245. What happens to DNA in the presence of a chaotropic salt? - (reply: 3)
  246. Quantify gDNA in buccal swab - (reply: 2)
  247. A substitute for Triton-X100 in DNA extraction - (reply: 9)
  248. Plasmid DNA at RT for a few days - (reply: 4)
  249. Control primers for bisulfite-converted DNA - (reply: 3)
  250. impurities after DNA extraction with promega kit!!!!!!&# - (reply: 6)
  251. DNA amplification differences between samples & between regions (WGA & s - (reply: 2)
  252. Low DNA concentration from mini-preps - (reply: 3)
  253. lipofectamine 2000:DNA complex stability - (reply: 2)
  254. Best method for extraction on low concentration environmental DNA? - (reply: 2)
  255. Ethanol precipitation of DNA. How long...? - (reply: 3)
  256. Genomic DNA extraction - how to quantify for PCR? - (reply: 9)
  257. separate plant protoplast from bacteria‏ prior DNA extraction - (reply: 3)
  258. DNA Kit Stability - (reply: 4)
  259. DNA and siRNA transfection and cytotoxicity - (reply: 1)
  260. suitable DNA extraction and sample freezing for MSP - (reply: 1)
  261. PhiX174 DNA - (reply: 1)
  262. Purity problems in DNA extracted from stool samples - (reply: 3)
  263. DNA or cDNA? - (reply: 1)
  264. Where can I purchase T2 or T4 phage genomic DNA? - (reply: 2)
  265. Extraction of insect mitochondrial DNA - (reply: 2)
  266. How many ug of DNA per ChIP is recommendable. - (reply: 5)
  267. DNA extraction for MCF-7 and HeLa - (reply: 4)
  268. About Methylated & Non-methylated pUC19 DNA Set - (reply: 1)
  269. DNA extraction from Fin Clips - (reply: 4)
  270. DNA pellet disappeared after ethanol wash - (reply: 3)
  271. Checking DNase activity - (reply: 3)
  272. DNA lost dramatically during storage - (reply: 5)
  273. Synthesis of carrier RNA(poly-A) for DNA Extraction - (reply: 2)
  274. Mycobacterium DNA Dilution for Quantification - (reply: 2)
  275. Sterile Air Drying of DNA - (reply: 3)
  276. DNA pellet - (reply: 2)
  277. DNA Isolation from Mouse Tissue - (reply: 1)
  278. no DNA fragment pull down in the first CpG island (MBD-seq) - (reply: 2)
  279. problems with recovery of dna from gel and ligation - (reply: 5)
  280. Chromosomal DNA? - (reply: 5)
  281. DNA of mitochondria - - (reply: 1)
  282. mitochondrial DNA - (reply: 7)
  283. Effect of varying DNA concentration levels in methylation analysis - (reply: 2)
  284. Dissolving DNA - finding better techniques - (reply: 12)
  285. Cell Fixation for DNA flow cytometry in fibroblasts - (reply: 2)
  286. What gel percent and Voltage is needed to separate 2680bp and 2600bp DNA nicely - (reply: 7)
  287. Dilution of Stock DNA - (reply: 4)
  288. Why do i get DNA of low concentrate after purification with kit - (reply: 9)
  289. DNA sequencing after ligation! HELP! - (reply: 8)
  290. Making DNA Linkers for cloning purposes - (reply: 1)
  291. Quantify DNA before ChIP PCR - (reply: 3)
  292. can we extract DNA from snap freeze tissue - (reply: 2)
  293. About Hot Start Taq DNA Polymerases - (reply: 2)
  294. Question about PCR - DNA or RNA as template - (reply: 4)
  295. cloning short genomic DNA fragments - (reply: 1)
  296. Incorporating RNAse A into DNA extraction - (reply: 2)
  297. ethanol in final DNA sample - (reply: 1)
  298. Uncut whole genomic DNA running too high - (reply: 1)
  299. Primers amplify genomic DNA but not cDNA - (reply: 2)
  300. Clean genomic DNA and plasmid DNA extraction from E.coli - (reply: 3)
  301. How to extract DNA from small number of cells - (reply: 2)
  302. Ethanol Precipitation ChIP DNA troubleshoot - (reply: 1)
  303. digestion and labelling of large ammount of DNA - (reply: 2)
  304. DNA extraction from varnished insect bodies - (reply: 2)
  305. genomic DNA extraction from formalin fixed tissues (not embedded) for genotyping - (reply: 4)
  306. Is the genomic DNA similar among different cell lines? - (reply: 4)
  307. Degraded DNA from Mouse Tissues - (reply: 5)
  308. How getting truncated DNA fragments? - (reply: 7)
  309. Genotyping genomic DNA before DNase treatment? - (reply: 1)
  310. How to concentrate DNA - (reply: 8)
  311. annotate mRNA and gDNA seq, Identify 5' and 3' untraslated regions, Iden - (reply: 2)
  312. SDS precipitation after adding DNA binding buffer for column purification - (reply: 1)
  313. Recommended tamplate DNA concentration for qPCR - (reply: 2)
  314. Plasmid prep - no DNA pellet after ethanol precipitation - (reply: 4)
  315. How many fragments in plasmid DNA prep? - (reply: 1)
  316. DNA extraction from Potato Chips - (reply: 6)
  317. MW of PEG for Purification of DNA - (reply: 2)
  318. Enough checks for gDNA contamination in qPCR? - (reply: 3)
  319. HiTrap Heparin HP for purification of DNA binding proteins. - (reply: 1)
  320. sample of choice for non-tumor DNA - (reply: 5)
  321. DNA extration from bacteria using kits or conventional methods - (reply: 6)
  322. DNA fragmentation - (reply: 1)
  323. Denaturation of DNA blotted - (reply: 1)
  324. very low concentraion of DNA from Bacteria - (reply: 3)
  325. DNA dissappearing during SAP incubation - (reply: 3)
  326. what is your DNA concentration after gel extraction? - (reply: 5)
  327. gDNA Isolation from Human Breast Tissue - (reply: 2)
  328. Saliva and GCF DNA purification - (reply: 1)
  329. DNA sequence of Herpes - (reply: 2)
  330. Remove HA Tag in DNA sequence! Help - (reply: 1)
  331. DNA isolation/purification troubleshooting. - (reply: 5)
  332. Colony PCR works but DNA yeild very low after miniprep - (reply: 2)
  333. No bands appearing in DNA gel electrophoresis - (reply: 1)
  334. Conversion of ng/ul of DNA to ng for a PCR reaction - (reply: 2)
  335. Denatured ethanol and DNA preservation - (reply: 2)
  336. DNA in methylation analysis - (reply: 3)
  337. Shearing issue: always having unsheared DNA (Please help!) - (reply: 9)
  338. multiple bands for genomic DNA extraction - (reply: 3)
  339. Getting genomic DNA for PCR - (reply: 4)
  340. I canīt obtain the amount of DNA required for ligation of blunt ends - (reply: 2)
  341. Phase separation during DNA extraction - (reply: 5)
  342. Digestion of Genomic DNA - (reply: 1)
  343. Are hybridoma germline DNA and hyrbidoma cDNA sequences identical? - (reply: 2)
  344. Repurifying DNA - (reply: 3)
  345. How to dilute the template DNA for PCR - (reply: 7)
  346. After I run my pcr on agarose the DNA is still in the well - (reply: 3)
  347. unespecific band PCR from mouse genomic DNA - (reply: 9)
  348. Extracting DNA from fruits and Vegetables - (reply: 8)
  349. Does DNase work at 8 degree? - (reply: 1)
  350. DNA quantification - (reply: 1)
  351. Isolation of both DNA and RNA from the same plant tissue - (reply: 8)
  352. Vertical agarose gel electrophoresis for DNA - (reply: 1)
  353. I forgot treat my samples with proteinase K before the DNA extraction step - (reply: 1)
  354. DNA Construction: HIV-1 Tat Gene - (reply: 9)
  355. mitochondrial DNA extraction from avian blood -urgent - (reply: 5)
  356. Cotransient Transfections DNA Requirements - (reply: 3)
  357. Generating stable cell line with linear DNA - (reply: 3)
  358. generating small DNA fragments with stick ends by annealing oligos? - (reply: 1)
  359. Weird results for gDNA extraction... - (reply: 5)
  360. precipitation of RNA after DNase treatment - (reply: 3)
  361. Smear above plasmid dna - (reply: 3)
  362. restriction digestion buffers contaminated with dnase - (reply: 3)
  363. Can we use denatured DNA standards on RNA formamide gels - (reply: 2)
  364. plotting DNA methylation profile vs TSS - (reply: 1)
  365. No DNA after midiprep - (reply: 2)
  366. problem in expressing A DNA binding protein - (reply: 6)
  367. Troubleshooting for DNA purification - (reply: 1)
  368. RNA immunoprecipitation DNase treatment - (reply: 4)
  369. Weight of a single Lambda DNA. - (reply: 3)
  370. cDNA or genomic DNA for over expression in Arabidopsis - (reply: 1)
  371. PCR failure from yeast genomic DNA - (reply: 1)
  372. failure of PCR from yeast genomic DNA - (reply: 1)
  373. Problem: Cloning DNA shuffled band - (reply: 2)
  374. troubles in DNA digestion - (reply: 7)
  375. Does DNA have to be supercoiled for transient protein expression? - (reply: 4)
  376. Colony lift hybridisation positive but clones are negative for insert DNA - (reply: 1)
  377. How to clean extracted faecal DNA? (removing PCR inhibitors) - (reply: 1)
  378. dna extraction from plant tissue infected with fungus - (reply: 1)
  379. DNA Storage - (reply: 10)
  380. Quiagen DNA extraction kit problem - (reply: 3)
  381. DNA extraction from cerebrospinal fluid using trizol - (reply: 2)
  382. Problem with restriction digestion of agarose-embedded DNA - (reply: 4)
  383. RT-PCR inhibited after Turbo DNase treatment - (reply: 3)
  384. isolation of genomic DNA from chicken blood in conventional method - (reply: 3)
  385. DNA and RNA extraction - (reply: 6)
  386. ChIP on episomal DNA - (reply: 2)
  387. DNA ladder running faster than samples during agarose gel electrophoresis - (reply: 1)
  388. isopropanol DNA precipitation - (reply: 1)
  389. That is DNA breathing? - (reply: 4)
  390. How to add restriction enzyme site into the DNA fragment - (reply: 3)
  391. isolation of genomic bacterial DNA (bacillus) - (reply: 2)
  392. DNA extraction and paraffin embedded tissue - (reply: 5)
  393. PAGE for Separation of DNA - (reply: 3)
  394. Template DNA (plasmid) concentration for PCR - (reply: 4)
  395. How to determine the volume of lisis buffer and DNA extraction buffer when the a - (reply: 5)
  396. How to find that my RNA is not contaminated with gDNA, before running Real-Time - (reply: 5)
  397. No band after extracting DNA from bacillus subtilis - (reply: 1)
  398. Bacillus subtilis DNA extraction protocol - (reply: 7)
  399. dna sequencing time - (reply: 1)
  400. Highly Variable DNA yield from Neutrophils lysed & sheared for ChIP - (reply: 7)
  401. Ammonium acetate DNA extraction mistake - (reply: 1)
  402. cell DNA content of cells grown on different scaffolds - (reply: 2)
  403. no dna after extraction - (reply: 5)
  404. Can I re-precipitate plasma DNA in TE? - (reply: 3)
  405. restricted DNA from the enzymes Bam H1, Hind111, Eco R1 and Sau 3A fall in? - (reply: 1)
  406. How do I insert a DNA sequence into a multiple cloning site and add restriction - (reply: 1)
  407. Strange PCR result from genomic DNA - (reply: 3)
  408. Protein contamination with DNA - (reply: 5)
  409. Quantifying DNA from Buccal Swabs - (reply: 1)
  410. help in digesting DNA fragment using restriction enzyme - (reply: 2)
  411. DNA Electrophoresis and SB (sodium borate) Buffer - (reply: 3)
  412. DNA methylation of a promoter region in different age groups - (reply: 2)
  413. Dilution, Cell concentration & DNA content - (reply: 3)
  414. RNAse treatment of genomic DNA - concentration and duration? - (reply: 1)
  415. turbid DNA from maxiprep - (reply: 3)
  416. DNA sequence data came back with lots of wrong bases - (reply: 5)
  417. synthetic DNA - (reply: 1)
  418. Isolating Genomic DNA from a small colony of cultured human cells - (reply: 1)
  419. Help: I am loosing the DNA during PCR purification - (reply: 7)
  420. PCR primer with no extra DNA end - (reply: 5)
  421. Protein contamination - How to remove all protein from my DNA? - (reply: 3)
  422. Extracting DNA from FACS sorted cells - (reply: 3)
  423. Didint get any band during gel electrophoresis even though DNA was there - (reply: 5)
  424. syto9 for DNA gel electrophoresis - (reply: 1)
  425. Environmental DNA extractions - (reply: 2)
  426. Help with DNA acrylamide gel - leaking and errors during gel running - (reply: 4)
  427. In a pcr why doesnt the DNA reneal during annealing process ? - (reply: 2)
  428. Difference between plasmid DNA extraction and genomic DNA extraction - (reply: 3)
  429. Weird DNA electrophoresis result - (reply: 4)
  430. Gooey DNA is protein samples after adding sample buffer - (reply: 1)
  431. DNA extraction and blue dextran as a DNA carrier - (reply: 4)
  432. DNA degradation (from human blood) - (reply: 3)
  433. Getting Rid of DNA after Transcription - (reply: 1)
  434. DNA migrate differently in agarose vs PAGE gel - (reply: 4)
  435. DNA Concentration - (reply: 1)
  436. PCR products form with DNA, but not with c-DNA - (reply: 2)
  437. DNA polymerase: recombinant or native? - (reply: 3)
  438. To isolate DNA, RNA and Protein from same culture flask for methylation study? - (reply: 2)
  439. Bisulfite treatment of DNA follow by Sequencing - (reply: 2)
  440. Help to construct luciferase DNA construct - construct luciferase DNA construct (reply: 2)
  441. concentration - concentration of DNA (reply: 3)
  442. Random digest of double stranded DNA - (reply: 1)
  443. effect of DNA isolation and storage on methylation - (reply: 1)
  444. nested PCR with low target DNA? - (reply: 2)
  445. DNA precipitation - A general question about DNA precipitation (reply: 3)
  446. Purification of DNA after MeDIP and WGA - (reply: 1)
  447. DNA extraction of microalgae - microalgae (reply: 3)
  448. DNA polymerase with single G 3'-end overhang activity - (reply: 3)
  449. ~200kb Plasmid DNA purification is not working - (reply: 1)
  450. Plasmid DNA size - Plasmid DNA backbone size (reply: 1)
  451. DNA probe labeling for EMSA; How? - Long probe, not oligonucletides (reply: 1)
  452. DNA extract quality - (reply: 3)
  453. Stacking gel for DNA PAGE - (reply: 2)
  454. Newbie- choose DNA template - (reply: 1)
  455. DNA extraction from liver - (reply: 3)
  456. DNA extraction - we want to extract DNA from bone marrow smear (reply: 2)
  457. Ligating Incompatible DNA fragments - Ligating Incompatible DNA fragments (reply: 3)
  458. Lambda DNA ligation - (reply: 1)
  459. Difference between PCR/cloning DNA in plasmids - (reply: 2)
  460. What is the expected pattern of restriction digestion of human genomic DNA? - (reply: 2)
  461. DNA purity - (reply: 1)
  462. Genomic DNA contamination in my RNA sample? - (reply: 8)
  463. RNase should be added in which step for gDNA isolation using phenol chloroform m - after lysozyme / preteinase k treatment or after phenol chloroform? (reply: 9)
  464. Dna after gel extraction, single or double stranded? - (reply: 1)
  465. Whole blood RNA and DNA extraction help - Expression analysis and epigenetics (reply: 2)
  466. DNase treatment after RNA extraction using RNeasy kit of qiagen - DNaes treatment after RNA extraction (reply: 2)
  467. Stability of DNA at RT - (reply: 1)
  468. ChIP- Different DNA size for Input DNA and ChIP DNA on Agilent Bioanalyzer - (reply: 2)
  469. How to get rid of DNA/RNA in protein expression preps - Is there an alternative to DNAse? (reply: 1)
  470. Smear and fluorescent in the well, could it be DNA or ? - (reply: 1)
  471. no band observed for bacterial genomic DNA during agarose gel electrophoresis - (reply: 9)
  472. Gentle method to dry a DNA pellet after alcohol precipitation - (reply: 5)
  473. Extraction of 12 bp DNA from polyacrylamide gel - (reply: 2)
  474. dna with very low GC content? - (reply: 2)
  475. PCRing small region on genomic DNA - (reply: 3)
  476. ChIP on chondrogenic pellets -- help! - no DNA signal at all (reply: 4)
  477. dna contamination after turbo free dna - (reply: 2)
  478. Musca domestica gDNA isolation - (reply: 3)
  479. Funny DNA bands on Agarose gels - (reply: 6)
  480. Is DNAse I step necessary after RNA synthesis by in vitro transcription? - (reply: 1)
  481. Remove gDNA from RNA sample - (reply: 3)
  482. Why does freezing/thawing DNA samples first improve PCR results? - (reply: 8)
  483. How does a DNA binding column work - (reply: 3)
  484. smears in PCR products (bisfulite treated DNA) - (reply: 4)
  485. DNA Quantification - Problems with DNA Quantification (reply: 2)
  486. No DNA after PCR purification with QiaQuick? - (reply: 4)
  487. dna extraction question - (reply: 2)
  488. DNA extractio kit - (reply: 8)
  489. genomic dna isolation - (reply: 5)
  490. DNA denaturation and renaturation - (reply: 1)
  491. DNA FISH protocol, wondering what some of these steps are for - (reply: 3)
  492. DNA stored in Ethidium Bromide degrade easily? - From CsCl prep (reply: 1)
  493. PCR genomic DNA using cell lysate - (reply: 1)
  494. DNA purification from restriction digest - It keeps on degrading :( :( :( (reply: 4)
  495. Amount of DNA to send for sequencing - linear response in signal intenstiy? - (reply: 1)
  496. How is bisulfite converted DNA unstable over time? - (reply: 4)
  497. Putative tumor suppressor promoter heavily methylated yet upregulated by DNA dam - (reply: 3)
  498. Problem loading DNA / agarose gel - (reply: 14)
  499. DNA quantification of BAC clones - (reply: 1)
  500. Bacterial DNA Extraction - possible of contamination? - (reply: 7)
  501. smear in pcr +DNA polymerase mixture for long PCR - (reply: 10)
  502. 1-day old culture for DNA extraction - plausible? - (reply: 2)
  503. Using DNA as a reference in qRT-PCR - (reply: 1)
  504. Doing PCR on Nebulized DNA - (reply: 2)
  505. Centrifuge speed for DNA extraction - Help (reply: 4)
  506. RNA carrier when using not so good DNA for bisulfite conversion - (reply: 3)
  507. Genomic DNA purity...??? - strange 260/280 and 260/230 ratios (reply: 7)
  508. Question about RNA extraction & gDNA elimination - can you really eliminate gDNA & how do you like to do it? (reply: 1)
  509. Cleaning up old (not quite ancient) gDNA extractions - (reply: 5)
  510. Help! How much DNA to use in IP reaction? - (reply: 1)
  511. 3 distinct bands of gDNA on gel - (reply: 2)
  512. How rapid are DNA methylation changes? - (reply: 1)
  513. DNA extraction from E. coli - solutions needed? (reply: 6)
  514. Internal Control RNA or DNA - (reply: 4)
  515. Weird bands in standard PCR of gDNA and cDNA - (reply: 2)
  516. DNA pellet - Does not stick to the vial (reply: 18)
  517. DNA detection limit of the PCR - Calculating detection limits (reply: 4)
  518. Proportion of colonies containing DNA inserted at the cloning site - (reply: 1)
  519. What causes RNA/DNA to unbind from silica membrane? - (reply: 1)
  520. Can Qiagen columns remove genomic DNA? - Settling a debate. (reply: 6)
  521. can contaminating gDNA explain this? - (reply: 1)
  522. ClustalW question - on multiple alignment of DNA sequences (reply: 1)
  523. My DNA pellet doesn't dissolve - Overdried?? (reply: 3)
  524. DNA, Gel - Possible reasons for DNA Gel Smear (reply: 12)
  525. DNA amount calculation for PCR - (reply: 14)
  526. Is T4 DNA Polymerase so evil? Blunt End Cloning - (reply: 3)
  527. Question: Start with genomic DNA or mRNA for methylation analysis - (reply: 4)
  528. why cut plasmid DNA with Hind111 and EcoR1? - (reply: 1)
  529. siRNA DNA co-transfection - (reply: 3)
  530. Storing genomic DNA in 4C with PBS? - (reply: 2)
  531. Problems PCR-labeling DNA probes with DIG-dUTP - (reply: 3)
  532. Odd question. Can I use DNase to decontaminate Primers? - Primer decontamination (reply: 2)
  533. uncut plasmid dna vs linearized plasmid gel - (reply: 1)
  534. control DNA transformation- using pGEM kit - transformation (reply: 4)
  535. PCR for sequencing genomic DNA (multiple alleles) - Are there preferences for amplification of certain sequences? (reply: 1)
  536. PCR of complement genomic DNA - (reply: 3)
  537. Taq DNA polymerase - how can microbes affoard an error-making enzyme? (reply: 2)
  538. High Concentration DNA Extraction - how to get high conc without stickyness? (reply: 4)
  539. PCR for long and repetitive region from genomic DNA - (reply: 4)
  540. dna isolation - dna isolation (reply: 1)
  541. How to store plasmid DNA on filter paper - I want to make a folder of all of them (reply: 3)
  542. How do I PCR a DNA fragment with >200 CGG repeats? - (reply: 2)
  543. DNA and histone methylation help - (reply: 1)
  544. DNA extraction from plated colonies - (reply: 3)
  545. Transcription factors that dont bind DNA - (reply: 8)
  546. Sense and antisense DNA, and primer design - (reply: 6)
  547. Forward primer not working during DNA sequencing - DNA sequencing (reply: 10)
  548. DNA isolation - ethanol precipitation related (reply: 2)
  549. plasmid DNA versus genomic DNA - concentrations (reply: 1)
  550. Is DNase necessary for primers designed on the exon-exon boundary?? - (reply: 2)
  551. mirna overexpression/ library versus single construct/ unspecific pcr - unspecific band when pcr on gDNA from library but not on gDNA fron sin (reply: 2)
  552. Difficult to extract DNA from vulcanic coastal sediments - (reply: 2)
  553. Which acetat for a DNA isolation - (reply: 3)
  554. Is it possible to separate DNA with only 10bp difference? - (reply: 3)
  555. mini-prep plasmid isolation by alkaline lysis - genomic DNA vs plasmid DNA (reply: 6)
  556. Estimation of DNA Quantity on an Agarose Gel - (reply: 5)
  557. isolating 40bp DNA for recombination - (reply: 1)
  558. RT-PCR: DNA contamination, one vs two step - RNA work (reply: 11)
  559. white residue after DNA purification - (reply: 3)
  560. DNA probes for In Situ Hybridization - equivalence of Sense probe for control for Dig-DNA-label probe? (reply: 1)
  561. Human DNA fingerprinting - (reply: 19)
  562. Yeast Genomic DNA preparation - (reply: 4)
  563. genomic DNA extraction: up to 50kb? - genomic DNA isolation (reply: 1)
  564. About human DNA sequence identity - (reply: 6)
  565. Isoamyl Alcohol in DNA extraction - (reply: 3)
  566. DNA contamination of RNA samples for qPCR...weird results! - (reply: 3)
  567. need to know minimum amount of template DNA needed for pcr amplification - pcr (reply: 2)
  568. MaestroGen UltraSlim LED illuminator - for DNA gel (reply: 1)
  569. What's the highest DNA concentration for PCR? - before being inhibited? (reply: 7)
  570. Amplification of bisulfite converted gDNA for sequencing - (reply: 1)
  571. PCR amplification with Pfu / quality of DNA - (reply: 4)
  572. DNA extraction from paraffin embbed tissue - How can extract DNA form tissue (reply: 2)
  573. Genomic DNA electrophoresis? - (reply: 1)
  574. Saliva DNA extraction help - (reply: 3)
  575. Chip-seq blocking reagent - Samlon DNA is useful for Chip-chip (reply: 1)
  576. Chip-seq blocking reagent - Salmon DNA is useful for Chip-chip (reply: 1)
  577. Effect of a DNA prep on DE3 - (reply: 2)
  578. DNA Extraction from bacterial cultures - (reply: 3)
  579. DNA from ticks - (reply: 3)
  580. Using CDS DNA for expression - (reply: 3)
  581. Extraction of bacterial DNA - (reply: 1)
  582. DNA electrophoersis ladder very blurry - (reply: 4)
  583. isolate DNA from amniotic fluid - (reply: 1)
  584. DNA yields after Bisulfite Treatment - How can I optimize when I have low yield? (reply: 15)
  585. gDNA had smear band - problem in extraction human genomic DNA (reply: 2)
  586. Molecular Cloning od Recombinant DNA - (reply: 3)
  587. RAPD analysis with not that pure DNA - (reply: 4)
  588. Ethidium Bromide or safe dyes for DNA??? - (reply: 14)
  589. DNA extraction from induced phage - (reply: 1)
  590. DNA purification from blood - (reply: 6)
  591. Annealing DNA - (reply: 4)
  592. Cleaning contaminated DNA - separating human from bacterial DNA (reply: 1)
  593. Large DNA extraction from an agarose gel - (reply: 8)
  594. Dna cloning using NdeI/BamHI using pET11 vector - (reply: 4)
  595. DNA isolation from plant nuclei by plasmid extraction kit - (reply: 1)
  596. DNA isolation from human serum/plasma - (reply: 1)
  597. Viral DNA extraction - (reply: 1)
  598. gDNA restriction digest - (reply: 1)
  599. remove carbohydrate from DNA preps - (reply: 4)
  600. Adding Restriction Site to DNA - (reply: 3)
  601. DNA ext from paraffin-embedded tissue ... a dilemma ! - FFPE tissue refuse to lyse strange whitish clumps formed!! (reply: 1)
  602. genomic DNA extraction - why there appears a smear on the gel photo (reply: 11)
  603. cDNA convertion to DNA - (reply: 4)
  604. Cleaning RNase - How do i clean out the RNase from my genomic DNA (reply: 1)
  605. mite DNA extraction - (reply: 7)
  606. mite DNA extraction - (reply: 1)
  607. DNA molecular weight keeps changes - (reply: 3)
  608. SNP and DNA methylation - (reply: 1)
  609. regenerate qiagen midi columns - how to reuse the midiprep DNA columns (reply: 3)
  610. DNA stuck in gel well - some samples are stuck in the agarose-gel well and don't migrate (reply: 4)
  611. Generating Standard for qPCR - PCR vs plasmid DNA (reply: 1)
  612. salt for DNA precipitation - DNA purification (reply: 3)
  613. Eliminate bacteria(l DNA) from hypochlorite-treated nematode eggs - (reply: 2)
  614. DNA quantification after gel purification - (reply: 6)
  615. DNA sequencing question help - make up sequencing mastermix (reply: 1)
  616. G to A transition in bisulfite-treated DNA - (reply: 2)
  617. Why isnt Phenol miscible in Tris Buffer? - Isolation of Genomic DNA (reply: 1)
  618. Microtiter plate directed DNA degradation - Why is DNA on one plate degraded while intact on the next? (reply: 3)
  619. Whole Genome Amplification of bisulfite treated DNA - (reply: 7)
  620. DNA sample contaminated with lipids - (reply: 8)
  621. Problem with gel extraction of DNA. - (reply: 1)
  622. If a 20bp primer differs from template DNA by 20bp, can PCR work? - (reply: 1)
  623. How to clone DNA (already cloned in a vector) in to another vector - please help (reply: 20)
  624. genomic DNA isolation - (reply: 1)
  625. DNA product almost 1500 bp - (reply: 1)
  626. Slightly too high 260:280 and 260:230 ratios from gDNA extraction - Should I care? (reply: 2)
  627. DNA Sequencing / Cloning ? - (reply: 5)
  628. gDNA extraction with pestle and mortar without liquid nitrogen? - (reply: 5)
  629. Help with DNA Quantification - Cell lysis for DNA quantification using Hoechst 33258 (reply: 2)
  630. DNAse digestion while RNA extraction with TRIzol - anyone tried that before? (reply: 1)
  631. Amount of DNase to use (Qiagen) - (reply: 7)
  632. Restriction digestion of gDNA for phage library - (reply: 5)
  633. DNA precipitation- Why EtOH? - Why we use EtOH and NaOAc together? (reply: 1)
  634. How long can DNA precipitate at -20? - (reply: 6)
  635. electrophoresis/ difference between expected size and result/ what's the alt - How to confirm the band means the expected DNA? Sequencing&what else? (reply: 5)
  636. dna sequencing - (reply: 1)
  637. dna fragments - (reply: 3)
  638. internal control for genomic DNA - (reply: 1)
  639. DNA pooling for PCR - Saving money PCR (reply: 7)
  640. Sites for protein sequence from DNA sequence - (reply: 2)
  641. DNA zap - (reply: 1)
  642. problems when use qPCR to quantify bisulfite modified DNA - (reply: 4)
  643. direct lysis of cell pellet - How to get rid of DNA? (reply: 3)
  644. Basic DNA sequencing question - (reply: 5)
  645. measurement units of DNA - (reply: 2)
  646. measurement units of DNA - (reply: 4)
  647. DNA Extraction - (reply: 1)
  648. DNA bisulphited Amplification - the amplification dont produce any band!! (reply: 3)
  649. PCR genomic DNA of high GC content - (reply: 6)
  650. DNA extraction - (reply: 2)
  651. DNA extraction form endoscopy samples - (reply: 4)
  652. Functions of this reagents!!! - Please help me with DNA extraction!!! (reply: 1)
  653. Can we find out own germline DNA - (reply: 1)
  654. problem about DNA electrophoresis - the DNAs become two layers in the gel (reply: 4)
  655. DNA molar mass - (reply: 1)
  656. DNA loading buffer - (reply: 2)
  657. Left DNA plasmid out for a week - (reply: 3)
  658. PCR/DNA Extraction Problem - (reply: 5)
  659. Extraction of DNA smaller than 100 bp from Agarose Gel - (reply: 6)
  660. probably DNA is disturbing the quantification of my sample - (reply: 3)
  661. PCR of AT-rich DNA - (reply: 1)
  662. agarose gel of ligated DNA doesn't show any bands - (reply: 2)
  663. How to view 75 bp DNA in gel - (reply: 4)
  664. dna loading dye for proteins - (reply: 2)
  665. DNA extraction - (reply: 1)
  666. destroy DNA incorporated inside the pipettes - (reply: 5)
  667. Isobutanol extraction of DNA - (reply: 2)
  668. Looking for FASTA format of DNA of a gene amongst various species - (reply: 7)
  669. Can I leave DNA in agarose gel piece overnight at 4oC before extraction? - Can I leave DNA in agarose gel piece overnight at 4oC before extractio (reply: 2)
  670. DNA Precipitation - (reply: 2)
  671. DNA damage in gel - (reply: 8)
  672. Quantity of DNA - (reply: 3)
  673. converting OD 600 reading to DNA concentration - Totally having a brain hiccup (reply: 3)
  674. How much template DNA do you use for qPCR - (reply: 1)
  675. DNA isolation from dark hair - (reply: 4)
  676. Can a primer bind to DNA in a 5'-3 parallel fashion? - Just wondering... (reply: 1)
  677. Stability of genomic DNA in 1x PBS - (reply: 2)
  678. CLONING PROBLEM!!! - no DNA after ligation!!!!! (reply: 6)
  679. Problems ligating shRNA primers - Sequencing reveals damaged DNA (reply: 1)
  680. DNA degradation - (reply: 1)
  681. DNase 1 treratment of RNA - (reply: 11)
  682. NO DNA - Cant get DNA amount by spectrophotometry (reply: 1)
  683. DNA extraction from very small volumes - (reply: 5)
  684. dna extraction from soil samples - (reply: 4)
  685. Measuring DNA, RNA, protein and cell wall synthesis - (reply: 1)
  686. Plasmid DNA mini-prep - no good isolation (reply: 5)
  687. ChIP-chip - how much DNA do you label? equal amounts? (reply: 1)
  688. DNA fragmentation - (reply: 2)
  689. qPCR of BS DNA - (reply: 6)
  690. Real-Time PCR using genomic DNA (without DNA purification) - (reply: 3)
  691. Best method for validating DNA methylation array data - (reply: 1)
  692. Troubleshooting on DNA Extraction-Salt extraction - forgot to add SDS at the first step (reply: 1)
  693. DNA contamination in RNA samples - (reply: 2)
  694. DNA damage and repair - general questions about this subject (reply: 5)
  695. Fungal DNA extraction from wheat - (reply: 5)
  696. Glycogen - DNA precipitation? - (reply: 4)
  697. bisulfite sequencing cloning problem (methylation of DNa) - bisulfite sequencing cloning problem (methylation of DNa) (reply: 3)
  698. Precipitation in DNAse I incubation buffer - (reply: 1)
  699. DNA purification - DNA purification dissolved in water (reply: 3)
  700. purified dna using dh5alpha and qiaprep....plasmid not in genome - (reply: 3)
  701. What is the minimum DNA concentration requried for double digestion? - (reply: 1)
  702. Sample Grinding For DNA extraction - Sample Prep (reply: 3)
  703. Streaky DNA digest - (reply: 3)
  704. DNA extraction - why 100% and 70% ethanol? (reply: 13)
  705. DNA agarose gel electrophoresis - sum1 can teach me interpret this gel image.. (reply: 3)
  706. Help ADD 3 bases to DNA Sequences - (reply: 6)
  707. Qiagen Qiaquick vs MinElute PCR purification kit for ChIP DNA - (reply: 10)
  708. Freeze drying prior to DNA extraction - (reply: 1)
  709. Genomic DNA - (reply: 2)
  710. DNA sonication - RNA contamination? - New probe, start all over (reply: 13)
  711. DNA quantification: NanoDrop vs. Picogreen - measurement of ChIP Input DNA (reply: 9)
  712. Bad PCR, is it due to conditions, reagents, or lack of DNA template? - (reply: 1)
  713. DNA extraction from mouse tail - (reply: 2)
  714. DNA size markers run at different levels in the same gel - Same marker loaded in two lanes runs differently! (reply: 2)
  715. Is DNA an acid because of phosphate group - (reply: 2)
  716. partial DNA digestion formula - Linear DNA partial digestion (reply: 1)
  717. DNA and salt contamination using mirVANa miRNA isolation kit - (reply: 6)
  718. DNA Extraction - (reply: 2)
  719. Isolation of DNA from Scat - (reply: 2)
  720. genomic DNA isolation - (reply: 4)
  721. Equalizing RNA samples for Reverse Transcription - Should I do this before or after DNA digestion (reply: 2)
  722. Zymo EZ Methylation Gold DNA bisulphite treatment - No DNA present after treatment (reply: 5)
  723. DNA Extraction - maize ssr (reply: 3)
  724. cleaning sonicator tip to prevent DNA contaminationin samples - (reply: 1)
  725. why they use isopropanal for precipitate DNA in midiprep - (reply: 5)
  726. HELP with cell lysis for DNA assay - Something is floating in my lysate solution!!! (reply: 1)
  727. Amplification from plasmid DNA, but not from genomic DNA with the same target re - (reply: 4)
  728. DNA/RNA extraction from sputum - (reply: 3)
  729. DNA extration from Bacteria spores - (reply: 13)
  730. dam methylated DNA in dam - straights - (reply: 1)
  731. Which Plasmid DNA for standard curve? - (reply: 5)
  732. DNA stains - pronunciation question (reply: 3)
  733. dna smear in electrophoresis - (reply: 6)
  734. does anyone have experience with pwo DNA polymerase for long PCR fragments ? - (reply: 2)
  735. HELP! Genomic DNA, RNA-size bands - (reply: 2)
  736. Cell Lysis for DNA extraction - Not every sample is successful (reply: 5)
  737. help needed: PCR a gene from genomic DNA - (reply: 2)
  738. Dual cross-linking thoughts - Agents to help analyse proteins indirectly bound to DNA (reply: 16)
  739. dna microarray - questions - (reply: 1)
  740. some Q's about dna microarray - (reply: 2)
  741. Genomic DNA Library - Problem of reactivity - Genomic DNA library screening with antibody (reply: 1)
  742. Storage of gel extracted DNA before ligation - (reply: 3)
  743. NICKED DNA - (reply: 2)
  744. dna storage buffer - (reply: 3)
  745. Amplification of human genomic DNA - (reply: 2)
  746. trouble amplifying 2.5kb product from genomic DNA - (reply: 3)
  747. Fungal DNA Isolation - Fungal DNA isolation without liquid nitrogen (reply: 1)
  748. Can I use genomic DNA to make a standard curve for qPCR - (reply: 5)
  749. very low ChIP'd DNA amount. - (reply: 1)
  750. Comparing cell # or DNA content - of pelleted cells (reply: 1)
  751. Help for some general DNA Extraction Questions - (reply: 5)
  752. how to check the quality of DNA? - (reply: 4)
  753. mtDNA - included in isolated gDNA? - (reply: 4)
  754. DNA precipitation - (reply: 3)
  755. plasmid DNA disappear in the minipre - (reply: 2)
  756. Purify ChIP DNA using Qiagen spin filter - (reply: 7)
  757. DNA contamination after RNA extraction, which kit works best? - (reply: 6)
  758. Binding and eluting a DNA binding column - (reply: 1)
  759. genomic dna isolated from blood is brown color?! - (reply: 4)
  760. Removal of RNA from DNA sample - (reply: 15)
  761. 3-colour labeling for the single argon-ion laser? - DNA + 2 antibodies (reply: 1)
  762. Only DNA ladder , No desired band in PCR - (reply: 4)
  763. low total/input DNA levels in ChIP work-up - (reply: 7)
  764. plasmid dna isolation - (reply: 1)
  765. DNA and RNA contamination in RT PCR water controls - (reply: 2)
  766. Long PCR and genomic DNA isolation problems - (reply: 2)
  767. DNA supercoiling on agarose gel -> reference needed - (reply: 1)
  768. DNA methylation analysis - direct sequencing gives CT peaks - (reply: 1)
  769. DNA extraction from FFPE? - (reply: 2)
  770. MYCOPLASMALOGY - MYCOPLASMA DNA EXTRACTION (reply: 7)
  771. T4 DNA polymerisation - elongation or removal? (reply: 3)
  772. Whole Genome Methylation Analysis Feasibility - Methylation analysis on DNA from rare cell populations (reply: 3)
  773. DNA Ladder Issues - DNA Ladder Agarose Gel (reply: 5)
  774. compound that block DNA replication in human cell - (reply: 1)
  775. Isolation of genomic DNA from microbial consortia - (reply: 2)
  776. EXtraction of DNA bands from acrylamide gel - (reply: 4)
  777. do small pieces of DNA form a helix - (reply: 2)
  778. Large Circular DNA - (reply: 1)
  779. best method for DNA extraction - (reply: 3)
  780. heredity of DNA methylation - (reply: 2)
  781. purity of genomic DNA, low 260/230? - (reply: 1)
  782. long DNA amplification - (reply: 11)
  783. DNA Laddering - (reply: 1)
  784. gDNA pcr product as standard for absolute quantification? - (reply: 1)
  785. purification of a large amount of DNA - (reply: 3)
  786. DNA ligation - (reply: 2)
  787. global DNA methylation quiagen kit - (reply: 14)
  788. DNA binding/EMSA question - question about controls in DNA binding/EMSA (reply: 4)
  789. Concentration of DNA for Transformation - (reply: 1)
  790. Request Genomic DNA of Burkholderia mallei - possible: collaboration or exchange DNA with pseudomallei culture (reply: 8)
  791. Attaching a double stranded DNA to a surface - (reply: 1)
  792. Another band after plasmid purificaiton - ran the plasmid DNA on a gel (reply: 2)
  793. DNA Question (T/F) - (reply: 4)
  794. what primers should I use for DNA sequencing? please help - (reply: 2)
  795. what primers should I use for DNA sequencing?? - (reply: 3)
  796. DNase and PMA/Iono - (reply: 1)
  797. plasmid DNA lab question - (reply: 11)
  798. Rescue my DNA extraction - (reply: 1)
  799. denaturation of dna - (reply: 1)
  800. PCR using genomic DNA - (reply: 2)
  801. Using analytical grade reagents for DNA extraction - (reply: 6)
  802. SOS....Genomic DNA extraction - Extraction from gram positive bacillus stearothermophilus (reply: 8)
  803. Optimal stock concentration for gDNA storage - (reply: 1)
  804. Manual reverse primer design for bisulfite traeted DNA - (reply: 4)
  805. Using DNA in gel extract kept at -20šC - (reply: 2)
  806. Help! Plasmid disappearing after mini-prep. - A260 shows good quality DNA, but nothing appears on gel. (reply: 3)
  807. Help with Phenol-Chloroform DNA Extraction - (reply: 3)
  808. Further DNA purification steps needed - Post DNAeasy Kit Purification (reply: 4)
  809. DNA yield estimation - (reply: 1)
  810. Testing primer specificity... - ...without gDNA of target organisms (reply: 6)
  811. Size of fusarium DNA - (reply: 1)
  812. size of fungal DNA - (reply: 3)
  813. Best way to desalt very salty, low yield DNA - (reply: 3)
  814. Source of DNA for a Reporter Construct - How to PCR the promoter region of interest? (reply: 1)
  815. weak ChIP signal - suggestions to increase the amount of DNA? (reply: 10)
  816. DNA methylated PCR - (reply: 2)
  817. Why can't genomic DNA be stored at 2-8 for long periods? - (reply: 10)
  818. Genomic DNA from Blood Kits - (reply: 2)
  819. Direct PCR (PCR without DNA extraction) - (reply: 2)
  820. DNA concentration and Genome - (reply: 1)
  821. DNA ladders on a northern blot - (reply: 1)
  822. Accidently heated up the wrong DNA. Damage? - (reply: 2)
  823. genomic DNA quality for genome walking - (reply: 2)
  824. Large Molecular weight DNA Extarction - (reply: 7)
  825. weak bands for methylated DNA - (reply: 4)
  826. Amplfication of fungal DNA - (reply: 8)
  827. No amplification in PCR using gDNA extracted from formailin fixed paraffin embed - (reply: 3)
  828. dna quantification and quality assesement - abs 260nm / abs 280 nm <0.5 (reply: 2)
  829. optimal template dna concentrations - (reply: 1)
  830. Volume of DNA to add to PCR Master Mix? - (reply: 1)
  831. how much DNA you need for real time pcr after chip - CHIP (reply: 1)
  832. RNA Purification - After DNase (reply: 3)
  833. How To Fix NaOH/Tris DNA Digest Error? - (reply: 1)
  834. DNA extraction with expired kit? - (reply: 1)
  835. protein/DNA Sequence Alignment - (reply: 1)
  836. PCR with genomic dna - (reply: 9)
  837. DNA Enrichment for Methylation Analysis - (reply: 8)
  838. Finding corresponding amino acid in DNA sequence - (reply: 1)
  839. Buffer for 5 Kb DNA fragments - (reply: 2)
  840. Random library using TOPO vector - random genomic DNA ligated into TOPO, false inserts? (reply: 3)
  841. Plasmid DNA sequence and map - PLASMID DNA (reply: 2)
  842. DNA isolation - parasite - Cryptosporidium DNA isolation (reply: 3)
  843. pcr doesnt show any band for DNa walking - (reply: 8)
  844. DNA polymerases for PCR-sequencing - (reply: 11)
  845. help-how to extract fluo-labeled DNA from agarose gel - (reply: 1)
  846. Integrity of treated DNA - Is quality of bisulfite treated DNA poor? (reply: 1)
  847. Qiagen DNAeasy blood&tissue DNA isolation kit - (reply: 1)
  848. 5-AZA, Reversing DNA methylation (epigenetic reactivation experiments) - (reply: 3)
  849. Best method to send DNA? - (reply: 4)
  850. protein precipitation with TCA for dna extraction? - (reply: 1)
  851. no DNA after IP - (reply: 1)
  852. Trouble with PCR on genomic tomato DNA (I've tried many fixes) - (reply: 5)
  853. Best way to extract plasmid from Mycobacteria - Extraction of DNA (reply: 7)
  854. purefication and amplification of serum free DNA - (reply: 1)
  855. dnase treatment before his-tag purification - (reply: 3)
  856. DNA extraction fron HSV-1 infected Vero cell - (reply: 2)
  857. Freezing DNA have effects on real-time PCR efficiencies - (reply: 1)
  858. low DNA yield after extraction - (reply: 1)
  859. protein precipitation without phenol/chloroform for dna extraction? - (reply: 1)
  860. Is gDNA digestion before bisulfite conversion important? - (reply: 5)
  861. Agarose: DNA/dye mixture doesn't stay in the gel? - Thanks for any and all help received! =] (reply: 12)
  862. DNA isolation from tissue !!SOS!! - (reply: 1)
  863. GC rich DNA sequencing - (reply: 3)
  864. Gemonic Library - Deletion/Reduction of Chloro/Mit DNA (reply: 1)
  865. Plasmid DNA isolation - neutralisation of DNA (reply: 5)
  866. transcription - quality of DNA (reply: 1)
  867. Sterilize DNA preps - (reply: 5)
  868. Transcription - Quality of DNA (reply: 1)
  869. DIG DNA PCR labeling problem - (reply: 6)
  870. Storage/Shipping of DNA at ambient temps - (reply: 2)
  871. how to get rid of DNA (in TE) contamination - protein and phenol removal (no kits please) (reply: 17)
  872. Trizol RNA/DNA extraction - (reply: 3)
  873. dna not running for electrophoresis - (reply: 9)
  874. nondenaturing PAGE to separate dna - (reply: 4)
  875. RNA isolation for real-time RT-PCR - forgot to inactivate the DNAse (reply: 4)
  876. failed PCR on DNA extract from blood - (reply: 6)
  877. Problem encountered when dosing my gDNA by spectrophotometry - Never experienced such thing before! (reply: 7)
  878. DNA methylation prediction in plant DNA - (reply: 2)
  879. Phenol - why different for DNA and RNA extraction? (reply: 9)
  880. concentration of dna - (reply: 3)
  881. DNA dissolved in DMSO instead of H2O - (reply: 2)
  882. PCR contamination with human DNA first and now no bands!!! - (reply: 4)
  883. Questions regarding bisulfite treatment of DNA - (reply: 2)
  884. Fragmented DNA doesn't appear as bands on agarose gel - (reply: 5)
  885. Questions about 260/230 when reading DNA conc. - (reply: 1)
  886. Genomic DNA contamination of RNA - (reply: 24)
  887. dna separation in native PAGE - (reply: 3)
  888. DNA extraction and PCR advice requested - PCR, Contamination, Low abundance targets (reply: 7)
  889. Problem with DNA extraction from 1% agarose Gel - No DNA using Qiagen Qiaek 2 and Spin column kits (reply: 9)
  890. How stable is methylated DNA? - (reply: 4)
  891. dna concentration - please help (reply: 5)
  892. volume of DNA required (PCr product ) in agarose gel - (reply: 11)
  893. Restriction endonuclease - cut only double stand DNA? (reply: 4)
  894. separation of dna fragments in agarose gel electrophoresis - (reply: 7)
  895. Primer Design - Design Primer with DNA Sequence (reply: 1)
  896. Diluting DNA - (reply: 1)
  897. Dry Plant leaf samples for DNA extraction - (reply: 1)
  898. DNase I pre-treatment - how can i be sure that it was efficient??? (reply: 2)
  899. PCR without DNA extraction! - (reply: 2)
  900. dna degradation - (reply: 4)
  901. Gel shift with large DNA fragments (>1 kb) - Can I still do EMSA? (reply: 1)
  902. Filtration DNA before transfection? - (reply: 4)
  903. Genomic DNA and PCR - (reply: 4)
  904. DNAse activity in room temperature - (reply: 3)
  905. Can DNA be solubilized in hydrochloric acid? - (reply: 2)
  906. about the total RNA isolation (trizol methods) and further Dnase I - (reply: 3)
  907. Presence / lack of introns in genomic DNA of related species - (reply: 2)
  908. DNA cloning - (reply: 4)
  909. freezing of E.coli culture prior DNA prep - (reply: 5)
  910. EZ DNA Methylation kit - (reply: 1)
  911. DNA storage @ room temp on filter paper - (reply: 2)
  912. DNase I digestion - how long the fragments are at the end??? (reply: 3)
  913. Vortexing genomic DNA, detrimental or not? - (reply: 8)
  914. Transfection efficiency vs. amount of plasmid DNA - (reply: 1)
  915. DNA amplification but no bands on agarose gel, GC rich product, Roche - DNA amplification but no bands/smear in agarose gel (reply: 5)
  916. Genomic DNA copy number qPCR - Help with control (reply: 8)
  917. Why were proteins favoured over DNA as the genetic material before 1940? - University Genetics Past EXAM Question (reply: 2)
  918. Sources of Contamination in plasmid DNA Mini-Preps - (reply: 10)
  919. Genomic DNA contamination in RNA sample - (reply: 2)
  920. Isolation DNA and protein from a single sample - (reply: 5)
  921. DNA extraction from saliva/buccal swabs - (reply: 5)
  922. DNase I digestion - check if it worked?? (reply: 4)
  923. elution of dna from gel - (reply: 5)
  924. Question about DNA electrophoresis - (reply: 1)
  925. EZ DNA methyaltion kit...trouble shooting - (reply: 4)
  926. Could You Suggest me A good KIt for DNA extraction from Yeast? - (reply: 1)
  927. DNA extraction trouble - (reply: 7)
  928. DNA vs Protein Stains - Are there any that don't stain BOTH? (reply: 1)
  929. Extraction of large size genomic DNA - (reply: 3)
  930. Colored DNA after phenol extraction - Strange color in aqueous phase and degraded DNA... (reply: 1)
  931. CHIP - DNA concentration after sonication - (reply: 6)
  932. DNA Question for TV Show - (reply: 4)
  933. Principles behind DNA RNA protein isolation and purification - (reply: 2)
  934. Phenol chloroform extraction of DNA - (reply: 3)
  935. Shrimp nuclease to eliminate genomic DNA - (reply: 7)
  936. gDNA or cDNA amplification in RT-PCR - (reply: 3)
  937. suggestions on the genomic DNA quality - (reply: 2)
  938. ChIP DNA quality - problem with ChIP-qPCR (reply: 5)
  939. Dnase digestion and PCR - (reply: 4)
  940. Why is it necessary to add Taq DNA polymerase last during PCR? - (reply: 8)
  941. Promoter and genomic DNA - (reply: 2)
  942. Genomic DNA - (reply: 9)
  943. is there DNA ? - how to know ? (reply: 8)
  944. DNA digestion - Restriction Enzymes (reply: 3)
  945. Problem with DNA purification from agarose gel - (reply: 3)
  946. T4 DNA Ligase & Restriction Enzyme Expression - I am looking to save money! (reply: 4)
  947. DNA extration of pieces of human hearts - (reply: 2)
  948. DNA concentration from ultraspectrophotometer is not accurate - (reply: 6)
  949. RNA appearance on 1.5% gel after DNase I digestion - (reply: 2)
  950. DNA precipitation - (reply: 5)
  951. DNA folding to form the loop - (reply: 2)
  952. DNA purification - long fragments wanted - (reply: 1)
  953. Unidentified DNA bands - (reply: 1)
  954. Any good way to remove DNase? - (reply: 5)
  955. DNA shearing with Misonix 4000 and microplates cup horn - (reply: 2)
  956. Accidentally digested DNA overnight - still OK? - (reply: 4)
  957. dna for real time - (reply: 3)
  958. Remove RNA after DNA mini-prep - (reply: 1)
  959. Confirm DNA enrichment of methylation array - (reply: 2)
  960. Capillary DNA Sequencing - (reply: 6)
  961. can we use one-step RT-PCR kits to amplify DNA? - (reply: 1)
  962. Why is Cleavage of DNA using restrcition enzyme more suitable for genetic engine - !! confused confused confused !! (reply: 4)
  963. How pure is my DNA sample?? - Confused-need help urgently please! (reply: 1)
  964. unmethylaten DNA control - ?????????? (reply: 3)
  965. Tips for genomic DNA extraction from gram -ve bacteria - (reply: 2)
  966. M.SssI treatment of DNA - (reply: 1)
  967. Gel purification of vector DNA - low yield - (reply: 26)
  968. DNA IDENTIFICATION - HOW TO TELL THE DIFFERENCE (reply: 14)
  969. DNA extraction methods - PCI extrction (reply: 3)
  970. Plant DNA extraction - (reply: 5)
  971. Seperate DNA spin columns - (reply: 3)
  972. DNA quantification - (reply: 5)
  973. Digest genomic (eukaryote) DNA before running PCR, is it necessary? - I do not get any band from PCR using genomic DNA and different primers (reply: 6)
  974. Problem with DIG High Prime DNA Labeling and Detection Starter Kit II - (reply: 2)
  975. PCR from a smear genomic DNA ? - (reply: 5)
  976. DNA/RNA into nucleotides - (reply: 6)
  977. Fast, cheap and dummy proof large scale DNA extraction? - (reply: 6)
  978. difference between a linear and circular plasmid/DNA for stable transfections - will a circular plasmid stably integrate into the genome? (reply: 4)
  979. Help with Purifying DNA Hairpin - (reply: 2)
  980. Reuse DNA spin column - (reply: 22)
  981. Does UV cause DNA fragmentation? - how long do I need to put cells under UV to see fragmentation? (reply: 12)
  982. Best storage temperature for samples - serum, protein, tissue, DNA, RNA - Archives (reply: 7)
  983. Does phenol extraction have effect on DNA methylation? - (reply: 4)
  984. Selection during Extraction: Plasmid or Genomic DNA? - (reply: 1)
  985. RNase free syringe for Qiagen DNase treatment, could not find any company provid - (reply: 2)
  986. gDNA purification - anything better than alcohol precipitation? (reply: 13)
  987. Effect of freezing on DNA methylation? - (reply: 6)
  988. DNA sequencing analysis - DNA sequencing analysis (reply: 1)
  989. DNA sequencing analysis - DNA sequencing analysis (reply: 11)
  990. qPCR quantification of ChIP DNA - (reply: 2)
  991. In vivo transfection of plasmid DNA, how? - intramuscular injection DNA (reply: 2)