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DNA Related Discussions
  1. Denatured ethanol and DNA preservation - (reply: 2)
  2. DNA in methylation analysis - (reply: 1)
  3. Shearing issue: always having unsheared DNA (Please help!) - (reply: 9)
  4. multiple bands for genomic DNA extraction - (reply: 2)
  5. Getting genomic DNA for PCR - (reply: 4)
  6. Phase separation during DNA extraction - (reply: 5)
  7. Digestion of Genomic DNA - (reply: 1)
  8. Are hybridoma germline DNA and hyrbidoma cDNA sequences identical? - (reply: 2)
  9. Repurifying DNA - (reply: 3)
  10. How to dilute the template DNA for PCR - (reply: 7)
  11. After I run my pcr on agarose the DNA is still in the well - (reply: 3)
  12. unespecific band PCR from mouse genomic DNA - (reply: 9)
  13. Extracting DNA from fruits and Vegetables - (reply: 8)
  14. Does DNase work at 8 degree? - (reply: 1)
  15. DNA quantification - (reply: 1)
  16. Isolation of both DNA and RNA from the same plant tissue - (reply: 7)
  17. Vertical agarose gel electrophoresis for DNA - (reply: 1)
  18. I forgot treat my samples with proteinase K before the DNA extraction step - (reply: 1)
  19. DNA Construction: HIV-1 Tat Gene - (reply: 9)
  20. mitochondrial DNA extraction from avian blood -urgent - (reply: 5)
  21. Cotransient Transfections DNA Requirements - (reply: 3)
  22. Generating stable cell line with linear DNA - (reply: 3)
  23. generating small DNA fragments with stick ends by annealing oligos? - (reply: 1)
  24. Weird results for gDNA extraction... - (reply: 5)
  25. precipitation of RNA after DNase treatment - (reply: 3)
  26. Smear above plasmid dna - (reply: 3)
  27. restriction digestion buffers contaminated with dnase - (reply: 3)
  28. Can we use denatured DNA standards on RNA formamide gels - (reply: 2)
  29. plotting DNA methylation profile vs TSS - (reply: 1)
  30. No DNA after midiprep - (reply: 2)
  31. problem in expressing A DNA binding protein - (reply: 1)
  32. Troubleshooting for DNA purification - (reply: 1)
  33. RNA immunoprecipitation DNase treatment - (reply: 4)
  34. Weight of a single Lambda DNA. - (reply: 3)
  35. PCR failure from yeast genomic DNA - (reply: 1)
  36. failure of PCR from yeast genomic DNA - (reply: 1)
  37. Problem: Cloning DNA shuffled band - (reply: 2)
  38. troubles in DNA digestion - (reply: 7)
  39. Does DNA have to be supercoiled for transient protein expression? - (reply: 4)
  40. Colony lift hybridisation positive but clones are negative for insert DNA - (reply: 1)
  41. How to clean extracted faecal DNA? (removing PCR inhibitors) - (reply: 1)
  42. dna extraction from plant tissue infected with fungus - (reply: 1)
  43. DNA Storage - (reply: 10)
  44. Quiagen DNA extraction kit problem - (reply: 3)
  45. DNA extraction from cerebrospinal fluid using trizol - (reply: 2)
  46. Problem with restriction digestion of agarose-embedded DNA - (reply: 4)
  47. RT-PCR inhibited after Turbo DNase treatment - (reply: 3)
  48. isolation of genomic DNA from chicken blood in conventional method - (reply: 3)
  49. DNA and RNA extraction - (reply: 6)
  50. ChIP on episomal DNA - (reply: 2)
  51. DNA ladder running faster than samples during agarose gel electrophoresis - (reply: 1)
  52. isopropanol DNA precipitation - (reply: 1)
  53. That is DNA breathing? - (reply: 4)
  54. How to add restriction enzyme site into the DNA fragment - (reply: 3)
  55. isolation of genomic bacterial DNA (bacillus) - (reply: 2)
  56. DNA extraction and paraffin embedded tissue - (reply: 5)
  57. PAGE for Separation of DNA - (reply: 3)
  58. Template DNA (plasmid) concentration for PCR - (reply: 4)
  59. How to determine the volume of lisis buffer and DNA extraction buffer when the a - (reply: 5)
  60. How to find that my RNA is not contaminated with gDNA, before running Real-Time - (reply: 5)
  61. No band after extracting DNA from bacillus subtilis - (reply: 1)
  62. Bacillus subtilis DNA extraction protocol - (reply: 7)
  63. dna sequencing time - (reply: 1)
  64. Highly Variable DNA yield from Neutrophils lysed & sheared for ChIP - (reply: 7)
  65. Ammonium acetate DNA extraction mistake - (reply: 1)
  66. cell DNA content of cells grown on different scaffolds - (reply: 2)
  67. no dna after extraction - (reply: 5)
  68. Can I re-precipitate plasma DNA in TE? - (reply: 3)
  69. restricted DNA from the enzymes Bam H1, Hind111, Eco R1 and Sau 3A fall in? - (reply: 1)
  70. How do I insert a DNA sequence into a multiple cloning site and add restriction - (reply: 1)
  71. Strange PCR result from genomic DNA - (reply: 3)
  72. Protein contamination with DNA - (reply: 5)
  73. Quantifying DNA from Buccal Swabs - (reply: 1)
  74. help in digesting DNA fragment using restriction enzyme - (reply: 2)
  75. DNA Electrophoresis and SB (sodium borate) Buffer - (reply: 3)
  76. DNA methylation of a promoter region in different age groups - (reply: 2)
  77. Dilution, Cell concentration & DNA content - (reply: 3)
  78. RNAse treatment of genomic DNA - concentration and duration? - (reply: 1)
  79. turbid DNA from maxiprep - (reply: 3)
  80. DNA sequence data came back with lots of wrong bases - (reply: 5)
  81. synthetic DNA - (reply: 1)
  82. Isolating Genomic DNA from a small colony of cultured human cells - (reply: 1)
  83. Help: I am loosing the DNA during PCR purification - (reply: 7)
  84. PCR primer with no extra DNA end - (reply: 5)
  85. Protein contamination - How to remove all protein from my DNA? - (reply: 3)
  86. Extracting DNA from FACS sorted cells - (reply: 3)
  87. Didint get any band during gel electrophoresis even though DNA was there - (reply: 5)
  88. syto9 for DNA gel electrophoresis - (reply: 1)
  89. Environmental DNA extractions - (reply: 2)
  90. Help with DNA acrylamide gel - leaking and errors during gel running - (reply: 4)
  91. In a pcr why doesnt the DNA reneal during annealing process ? - (reply: 2)
  92. Difference between plasmid DNA extraction and genomic DNA extraction - (reply: 3)
  93. Weird DNA electrophoresis result - (reply: 4)
  94. Gooey DNA is protein samples after adding sample buffer - (reply: 1)
  95. DNA extraction and blue dextran as a DNA carrier - (reply: 4)
  96. DNA degradation (from human blood) - (reply: 3)
  97. Getting Rid of DNA after Transcription - (reply: 1)
  98. DNA migrate differently in agarose vs PAGE gel - (reply: 4)
  99. DNA Concentration - (reply: 1)
  100. PCR products form with DNA, but not with c-DNA - (reply: 2)
  101. DNA polymerase: recombinant or native? - (reply: 3)
  102. To isolate DNA, RNA and Protein from same culture flask for methylation study? - (reply: 2)
  103. Bisulfite treatment of DNA follow by Sequencing - (reply: 2)
  104. Help to construct luciferase DNA construct - construct luciferase DNA construct (reply: 2)
  105. concentration - concentration of DNA (reply: 3)
  106. Random digest of double stranded DNA - (reply: 1)
  107. effect of DNA isolation and storage on methylation - (reply: 1)
  108. nested PCR with low target DNA? - (reply: 2)
  109. DNA precipitation - A general question about DNA precipitation (reply: 3)
  110. Purification of DNA after MeDIP and WGA - (reply: 1)
  111. DNA extraction of microalgae - microalgae (reply: 3)
  112. DNA polymerase with single G 3'-end overhang activity - (reply: 3)
  113. ~200kb Plasmid DNA purification is not working - (reply: 1)
  114. Plasmid DNA size - Plasmid DNA backbone size (reply: 1)
  115. DNA probe labeling for EMSA; How? - Long probe, not oligonucletides (reply: 1)
  116. DNA extract quality - (reply: 2)
  117. Stacking gel for DNA PAGE - (reply: 2)
  118. Newbie- choose DNA template - (reply: 1)
  119. DNA extraction from liver - (reply: 3)
  120. DNA extraction - we want to extract DNA from bone marrow smear (reply: 2)
  121. Ligating Incompatible DNA fragments - Ligating Incompatible DNA fragments (reply: 3)
  122. Lambda DNA ligation - (reply: 1)
  123. Difference between PCR/cloning DNA in plasmids - (reply: 2)
  124. What is the expected pattern of restriction digestion of human genomic DNA? - (reply: 2)
  125. DNA purity - (reply: 1)
  126. Genomic DNA contamination in my RNA sample? - (reply: 8)
  127. RNase should be added in which step for gDNA isolation using phenol chloroform m - after lysozyme / preteinase k treatment or after phenol chloroform? (reply: 9)
  128. Dna after gel extraction, single or double stranded? - (reply: 1)
  129. Whole blood RNA and DNA extraction help - Expression analysis and epigenetics (reply: 2)
  130. DNase treatment after RNA extraction using RNeasy kit of qiagen - DNaes treatment after RNA extraction (reply: 2)
  131. Stability of DNA at RT - (reply: 1)
  132. ChIP- Different DNA size for Input DNA and ChIP DNA on Agilent Bioanalyzer - (reply: 2)
  133. How to get rid of DNA/RNA in protein expression preps - Is there an alternative to DNAse? (reply: 1)
  134. Smear and fluorescent in the well, could it be DNA or ? - (reply: 1)
  135. no band observed for bacterial genomic DNA during agarose gel electrophoresis - (reply: 9)
  136. Gentle method to dry a DNA pellet after alcohol precipitation - (reply: 5)
  137. Extraction of 12 bp DNA from polyacrylamide gel - (reply: 2)
  138. dna with very low GC content? - (reply: 2)
  139. PCRing small region on genomic DNA - (reply: 3)
  140. ChIP on chondrogenic pellets -- help! - no DNA signal at all (reply: 4)
  141. dna contamination after turbo free dna - (reply: 2)
  142. Musca domestica gDNA isolation - (reply: 3)
  143. Funny DNA bands on Agarose gels - (reply: 6)
  144. Is DNAse I step necessary after RNA synthesis by in vitro transcription? - (reply: 1)
  145. Remove gDNA from RNA sample - (reply: 3)
  146. Why does freezing/thawing DNA samples first improve PCR results? - (reply: 8)
  147. How does a DNA binding column work - (reply: 3)
  148. smears in PCR products (bisfulite treated DNA) - (reply: 2)
  149. DNA Quantification - Problems with DNA Quantification (reply: 2)
  150. No DNA after PCR purification with QiaQuick? - (reply: 4)
  151. dna extraction question - (reply: 2)
  152. DNA extractio kit - (reply: 8)
  153. genomic dna isolation - (reply: 5)
  154. DNA denaturation and renaturation - (reply: 1)
  155. DNA FISH protocol, wondering what some of these steps are for - (reply: 3)
  156. DNA stored in Ethidium Bromide degrade easily? - From CsCl prep (reply: 1)
  157. PCR genomic DNA using cell lysate - (reply: 1)
  158. DNA purification from restriction digest - It keeps on degrading :( :( :( (reply: 4)
  159. Amount of DNA to send for sequencing - linear response in signal intenstiy? - (reply: 1)
  160. How is bisulfite converted DNA unstable over time? - (reply: 2)
  161. Putative tumor suppressor promoter heavily methylated yet upregulated by DNA dam - (reply: 3)
  162. Problem loading DNA / agarose gel - (reply: 11)
  163. DNA quantification of BAC clones - (reply: 1)
  164. Bacterial DNA Extraction - possible of contamination? - (reply: 7)
  165. smear in pcr +DNA polymerase mixture for long PCR - (reply: 10)
  166. 1-day old culture for DNA extraction - plausible? - (reply: 2)
  167. Using DNA as a reference in qRT-PCR - (reply: 1)
  168. Doing PCR on Nebulized DNA - (reply: 2)
  169. Centrifuge speed for DNA extraction - Help (reply: 4)
  170. RNA carrier when using not so good DNA for bisulfite conversion - (reply: 2)
  171. Genomic DNA purity...??? - strange 260/280 and 260/230 ratios (reply: 7)
  172. Question about RNA extraction & gDNA elimination - can you really eliminate gDNA & how do you like to do it? (reply: 1)
  173. Cleaning up old (not quite ancient) gDNA extractions - (reply: 5)
  174. Help! How much DNA to use in IP reaction? - (reply: 1)
  175. 3 distinct bands of gDNA on gel - (reply: 2)
  176. How rapid are DNA methylation changes? - (reply: 1)
  177. DNA extraction from E. coli - solutions needed? (reply: 6)
  178. Internal Control RNA or DNA - (reply: 4)
  179. Weird bands in standard PCR of gDNA and cDNA - (reply: 2)
  180. DNA pellet - Does not stick to the vial (reply: 18)
  181. DNA detection limit of the PCR - Calculating detection limits (reply: 3)
  182. Proportion of colonies containing DNA inserted at the cloning site - (reply: 1)
  183. What causes RNA/DNA to unbind from silica membrane? - (reply: 1)
  184. Can Qiagen columns remove genomic DNA? - Settling a debate. (reply: 6)
  185. can contaminating gDNA explain this? - (reply: 1)
  186. ClustalW question - on multiple alignment of DNA sequences (reply: 1)
  187. My DNA pellet doesn't dissolve - Overdried?? (reply: 3)
  188. DNA, Gel - Possible reasons for DNA Gel Smear (reply: 12)
  189. DNA amount calculation for PCR - (reply: 14)
  190. Is T4 DNA Polymerase so evil? Blunt End Cloning - (reply: 3)
  191. Question: Start with genomic DNA or mRNA for methylation analysis - (reply: 4)
  192. why cut plasmid DNA with Hind111 and EcoR1? - (reply: 1)
  193. siRNA DNA co-transfection - (reply: 3)
  194. Storing genomic DNA in 4C with PBS? - (reply: 2)
  195. Problems PCR-labeling DNA probes with DIG-dUTP - (reply: 3)
  196. Odd question. Can I use DNase to decontaminate Primers? - Primer decontamination (reply: 2)
  197. uncut plasmid dna vs linearized plasmid gel - (reply: 1)
  198. control DNA transformation- using pGEM kit - transformation (reply: 4)
  199. PCR for sequencing genomic DNA (multiple alleles) - Are there preferences for amplification of certain sequences? (reply: 1)
  200. PCR of complement genomic DNA - (reply: 3)
  201. Taq DNA polymerase - how can microbes affoard an error-making enzyme? (reply: 2)
  202. High Concentration DNA Extraction - how to get high conc without stickyness? (reply: 4)
  203. PCR for long and repetitive region from genomic DNA - (reply: 4)
  204. dna isolation - dna isolation (reply: 1)
  205. How to store plasmid DNA on filter paper - I want to make a folder of all of them (reply: 3)
  206. How do I PCR a DNA fragment with >200 CGG repeats? - (reply: 2)
  207. DNA and histone methylation help - (reply: 1)
  208. DNA extraction from plated colonies - (reply: 3)
  209. Transcription factors that dont bind DNA - (reply: 8)
  210. Sense and antisense DNA, and primer design - (reply: 6)
  211. Forward primer not working during DNA sequencing - DNA sequencing (reply: 10)
  212. DNA isolation - ethanol precipitation related (reply: 2)
  213. plasmid DNA versus genomic DNA - concentrations (reply: 1)
  214. Is DNase necessary for primers designed on the exon-exon boundary?? - (reply: 2)
  215. mirna overexpression/ library versus single construct/ unspecific pcr - unspecific band when pcr on gDNA from library but not on gDNA fron sin (reply: 2)
  216. Difficult to extract DNA from vulcanic coastal sediments - (reply: 2)
  217. Which acetat for a DNA isolation - (reply: 3)
  218. Is it possible to separate DNA with only 10bp difference? - (reply: 3)
  219. mini-prep plasmid isolation by alkaline lysis - genomic DNA vs plasmid DNA (reply: 6)
  220. Estimation of DNA Quantity on an Agarose Gel - (reply: 5)
  221. isolating 40bp DNA for recombination - (reply: 1)
  222. RT-PCR: DNA contamination, one vs two step - RNA work (reply: 11)
  223. white residue after DNA purification - (reply: 3)
  224. DNA probes for In Situ Hybridization - equivalence of Sense probe for control for Dig-DNA-label probe? (reply: 1)
  225. Human DNA fingerprinting - (reply: 19)
  226. Yeast Genomic DNA preparation - (reply: 4)
  227. genomic DNA extraction: up to 50kb? - genomic DNA isolation (reply: 1)
  228. About human DNA sequence identity - (reply: 6)
  229. Isoamyl Alcohol in DNA extraction - (reply: 3)
  230. DNA contamination of RNA samples for qPCR...weird results! - (reply: 3)
  231. need to know minimum amount of template DNA needed for pcr amplification - pcr (reply: 2)
  232. MaestroGen UltraSlim LED illuminator - for DNA gel (reply: 1)
  233. What's the highest DNA concentration for PCR? - before being inhibited? (reply: 7)
  234. Amplification of bisulfite converted gDNA for sequencing - (reply: 1)
  235. PCR amplification with Pfu / quality of DNA - (reply: 4)
  236. DNA extraction from paraffin embbed tissue - How can extract DNA form tissue (reply: 2)
  237. Genomic DNA electrophoresis? - (reply: 1)
  238. Saliva DNA extraction help - (reply: 3)
  239. Chip-seq blocking reagent - Samlon DNA is useful for Chip-chip (reply: 1)
  240. Chip-seq blocking reagent - Salmon DNA is useful for Chip-chip (reply: 1)
  241. Effect of a DNA prep on DE3 - (reply: 2)
  242. DNA Extraction from bacterial cultures - (reply: 3)
  243. DNA from ticks - (reply: 3)
  244. Using CDS DNA for expression - (reply: 3)
  245. Extraction of bacterial DNA - (reply: 1)
  246. DNA electrophoersis ladder very blurry - (reply: 4)
  247. isolate DNA from amniotic fluid - (reply: 1)
  248. DNA yields after Bisulfite Treatment - How can I optimize when I have low yield? (reply: 11)
  249. gDNA had smear band - problem in extraction human genomic DNA (reply: 2)
  250. Molecular Cloning od Recombinant DNA - (reply: 3)
  251. RAPD analysis with not that pure DNA - (reply: 4)
  252. Ethidium Bromide or safe dyes for DNA??? - (reply: 14)
  253. DNA extraction from induced phage - (reply: 1)
  254. DNA purification from blood - (reply: 6)
  255. Annealing DNA - (reply: 4)
  256. Cleaning contaminated DNA - separating human from bacterial DNA (reply: 1)
  257. Large DNA extraction from an agarose gel - (reply: 8)
  258. Dna cloning using NdeI/BamHI using pET11 vector - (reply: 4)
  259. DNA isolation from plant nuclei by plasmid extraction kit - (reply: 1)
  260. DNA isolation from human serum/plasma - (reply: 1)
  261. Viral DNA extraction - (reply: 1)
  262. gDNA restriction digest - (reply: 1)
  263. remove carbohydrate from DNA preps - (reply: 4)
  264. Adding Restriction Site to DNA - (reply: 3)
  265. DNA ext from paraffin-embedded tissue ... a dilemma ! - FFPE tissue refuse to lyse strange whitish clumps formed!! (reply: 1)
  266. genomic DNA extraction - why there appears a smear on the gel photo (reply: 11)
  267. cDNA convertion to DNA - (reply: 4)
  268. Cleaning RNase - How do i clean out the RNase from my genomic DNA (reply: 1)
  269. mite DNA extraction - (reply: 7)
  270. mite DNA extraction - (reply: 1)
  271. DNA molecular weight keeps changes - (reply: 3)
  272. SNP and DNA methylation - (reply: 1)
  273. regenerate qiagen midi columns - how to reuse the midiprep DNA columns (reply: 3)
  274. DNA stuck in gel well - some samples are stuck in the agarose-gel well and don't migrate (reply: 4)
  275. Generating Standard for qPCR - PCR vs plasmid DNA (reply: 1)
  276. salt for DNA precipitation - DNA purification (reply: 3)
  277. Eliminate bacteria(l DNA) from hypochlorite-treated nematode eggs - (reply: 2)
  278. DNA quantification after gel purification - (reply: 6)
  279. DNA sequencing question help - make up sequencing mastermix (reply: 1)
  280. G to A transition in bisulfite-treated DNA - (reply: 2)
  281. Why isnt Phenol miscible in Tris Buffer? - Isolation of Genomic DNA (reply: 1)
  282. Microtiter plate directed DNA degradation - Why is DNA on one plate degraded while intact on the next? (reply: 3)
  283. Whole Genome Amplification of bisulfite treated DNA - (reply: 7)
  284. DNA sample contaminated with lipids - (reply: 8)
  285. Problem with gel extraction of DNA. - (reply: 1)
  286. If a 20bp primer differs from template DNA by 20bp, can PCR work? - (reply: 1)
  287. How to clone DNA (already cloned in a vector) in to another vector - please help (reply: 20)
  288. genomic DNA isolation - (reply: 1)
  289. DNA product almost 1500 bp - (reply: 1)
  290. Slightly too high 260:280 and 260:230 ratios from gDNA extraction - Should I care? (reply: 2)
  291. DNA Sequencing / Cloning ? - (reply: 5)
  292. gDNA extraction with pestle and mortar without liquid nitrogen? - (reply: 5)
  293. Help with DNA Quantification - Cell lysis for DNA quantification using Hoechst 33258 (reply: 2)
  294. DNAse digestion while RNA extraction with TRIzol - anyone tried that before? (reply: 1)
  295. Amount of DNase to use (Qiagen) - (reply: 7)
  296. Restriction digestion of gDNA for phage library - (reply: 5)
  297. DNA precipitation- Why EtOH? - Why we use EtOH and NaOAc together? (reply: 1)
  298. How long can DNA precipitate at -20? - (reply: 6)
  299. electrophoresis/ difference between expected size and result/ what's the alt - How to confirm the band means the expected DNA? Sequencing&what else? (reply: 5)
  300. dna sequencing - (reply: 1)
  301. dna fragments - (reply: 3)
  302. internal control for genomic DNA - (reply: 1)
  303. DNA pooling for PCR - Saving money PCR (reply: 7)
  304. Sites for protein sequence from DNA sequence - (reply: 2)
  305. DNA zap - (reply: 1)
  306. problems when use qPCR to quantify bisulfite modified DNA - (reply: 4)
  307. direct lysis of cell pellet - How to get rid of DNA? (reply: 3)
  308. Basic DNA sequencing question - (reply: 5)
  309. measurement units of DNA - (reply: 2)
  310. measurement units of DNA - (reply: 4)
  311. DNA Extraction - (reply: 1)
  312. DNA bisulphited Amplification - the amplification dont produce any band!! (reply: 3)
  313. PCR genomic DNA of high GC content - (reply: 6)
  314. DNA extraction - (reply: 2)
  315. DNA extraction form endoscopy samples - (reply: 4)
  316. Functions of this reagents!!! - Please help me with DNA extraction!!! (reply: 1)
  317. Can we find out own germline DNA - (reply: 1)
  318. problem about DNA electrophoresis - the DNAs become two layers in the gel (reply: 4)
  319. DNA molar mass - (reply: 1)
  320. DNA loading buffer - (reply: 2)
  321. Left DNA plasmid out for a week - (reply: 3)
  322. PCR/DNA Extraction Problem - (reply: 5)
  323. Extraction of DNA smaller than 100 bp from Agarose Gel - (reply: 6)
  324. probably DNA is disturbing the quantification of my sample - (reply: 3)
  325. PCR of AT-rich DNA - (reply: 1)
  326. agarose gel of ligated DNA doesn't show any bands - (reply: 2)
  327. How to view 75 bp DNA in gel - (reply: 4)
  328. dna loading dye for proteins - (reply: 2)
  329. DNA extraction - (reply: 1)
  330. destroy DNA incorporated inside the pipettes - (reply: 5)
  331. Isobutanol extraction of DNA - (reply: 2)
  332. Looking for FASTA format of DNA of a gene amongst various species - (reply: 7)
  333. Can I leave DNA in agarose gel piece overnight at 4oC before extraction? - Can I leave DNA in agarose gel piece overnight at 4oC before extractio (reply: 2)
  334. DNA Precipitation - (reply: 2)
  335. DNA damage in gel - (reply: 8)
  336. Quantity of DNA - (reply: 3)
  337. converting OD 600 reading to DNA concentration - Totally having a brain hiccup (reply: 3)
  338. How much template DNA do you use for qPCR - (reply: 1)
  339. DNA isolation from dark hair - (reply: 4)
  340. Can a primer bind to DNA in a 5'-3 parallel fashion? - Just wondering... (reply: 1)
  341. Stability of genomic DNA in 1x PBS - (reply: 2)
  342. CLONING PROBLEM!!! - no DNA after ligation!!!!! (reply: 6)
  343. Problems ligating shRNA primers - Sequencing reveals damaged DNA (reply: 1)
  344. DNA degradation - (reply: 1)
  345. DNase 1 treratment of RNA - (reply: 8)
  346. NO DNA - Cant get DNA amount by spectrophotometry (reply: 1)
  347. DNA extraction from very small volumes - (reply: 5)
  348. dna extraction from soil samples - (reply: 4)
  349. Measuring DNA, RNA, protein and cell wall synthesis - (reply: 1)
  350. Plasmid DNA mini-prep - no good isolation (reply: 5)
  351. ChIP-chip - how much DNA do you label? equal amounts? (reply: 1)
  352. DNA fragmentation - (reply: 2)
  353. qPCR of BS DNA - (reply: 6)
  354. Real-Time PCR using genomic DNA (without DNA purification) - (reply: 3)
  355. Best method for validating DNA methylation array data - (reply: 1)
  356. Troubleshooting on DNA Extraction-Salt extraction - forgot to add SDS at the first step (reply: 1)
  357. DNA contamination in RNA samples - (reply: 2)
  358. DNA damage and repair - general questions about this subject (reply: 5)
  359. Fungal DNA extraction from wheat - (reply: 5)
  360. Glycogen - DNA precipitation? - (reply: 4)
  361. bisulfite sequencing cloning problem (methylation of DNa) - bisulfite sequencing cloning problem (methylation of DNa) (reply: 3)
  362. Precipitation in DNAse I incubation buffer - (reply: 1)
  363. DNA purification - DNA purification dissolved in water (reply: 3)
  364. purified dna using dh5alpha and qiaprep....plasmid not in genome - (reply: 3)
  365. What is the minimum DNA concentration requried for double digestion? - (reply: 1)
  366. Sample Grinding For DNA extraction - Sample Prep (reply: 3)
  367. Streaky DNA digest - (reply: 3)
  368. DNA extraction - why 100% and 70% ethanol? (reply: 13)
  369. DNA agarose gel electrophoresis - sum1 can teach me interpret this gel image.. (reply: 3)
  370. Help ADD 3 bases to DNA Sequences - (reply: 6)
  371. Qiagen Qiaquick vs MinElute PCR purification kit for ChIP DNA - (reply: 10)
  372. Freeze drying prior to DNA extraction - (reply: 1)
  373. Genomic DNA - (reply: 2)
  374. DNA sonication - RNA contamination? - New probe, start all over (reply: 13)
  375. DNA quantification: NanoDrop vs. Picogreen - measurement of ChIP Input DNA (reply: 9)
  376. Bad PCR, is it due to conditions, reagents, or lack of DNA template? - (reply: 1)
  377. DNA extraction from mouse tail - (reply: 2)
  378. DNA size markers run at different levels in the same gel - Same marker loaded in two lanes runs differently! (reply: 2)
  379. Is DNA an acid because of phosphate group - (reply: 2)
  380. partial DNA digestion formula - Linear DNA partial digestion (reply: 1)
  381. DNA and salt contamination using mirVANa miRNA isolation kit - (reply: 6)
  382. DNA Extraction - (reply: 2)
  383. Isolation of DNA from Scat - (reply: 2)
  384. genomic DNA isolation - (reply: 4)
  385. Equalizing RNA samples for Reverse Transcription - Should I do this before or after DNA digestion (reply: 2)
  386. Zymo EZ Methylation Gold DNA bisulphite treatment - No DNA present after treatment (reply: 5)
  387. DNA Extraction - maize ssr (reply: 3)
  388. cleaning sonicator tip to prevent DNA contaminationin samples - (reply: 1)
  389. why they use isopropanal for precipitate DNA in midiprep - (reply: 5)
  390. HELP with cell lysis for DNA assay - Something is floating in my lysate solution!!! (reply: 1)
  391. Amplification from plasmid DNA, but not from genomic DNA with the same target re - (reply: 4)
  392. DNA extration from Bacteria spores - (reply: 13)
  393. dam methylated DNA in dam - straights - (reply: 1)
  394. Which Plasmid DNA for standard curve? - (reply: 5)
  395. DNA stains - pronunciation question (reply: 3)
  396. dna smear in electrophoresis - (reply: 6)
  397. does anyone have experience with pwo DNA polymerase for long PCR fragments ? - (reply: 2)
  398. HELP! Genomic DNA, RNA-size bands - (reply: 2)
  399. Cell Lysis for DNA extraction - Not every sample is successful (reply: 5)
  400. help needed: PCR a gene from genomic DNA - (reply: 2)
  401. Dual cross-linking thoughts - Agents to help analyse proteins indirectly bound to DNA (reply: 14)
  402. dna microarray - questions - (reply: 1)
  403. some Q's about dna microarray - (reply: 2)
  404. Genomic DNA Library - Problem of reactivity - Genomic DNA library screening with antibody (reply: 1)
  405. Storage of gel extracted DNA before ligation - (reply: 3)
  406. NICKED DNA - (reply: 2)
  407. dna storage buffer - (reply: 3)
  408. Amplification of human genomic DNA - (reply: 2)
  409. trouble amplifying 2.5kb product from genomic DNA - (reply: 3)
  410. Fungal DNA Isolation - Fungal DNA isolation without liquid nitrogen (reply: 1)
  411. Can I use genomic DNA to make a standard curve for qPCR - (reply: 5)
  412. very low ChIP'd DNA amount. - (reply: 1)
  413. Comparing cell # or DNA content - of pelleted cells (reply: 1)
  414. Help for some general DNA Extraction Questions - (reply: 5)
  415. how to check the quality of DNA? - (reply: 4)
  416. mtDNA - included in isolated gDNA? - (reply: 3)
  417. DNA precipitation - (reply: 3)
  418. plasmid DNA disappear in the minipre - (reply: 2)
  419. DNA contamination after RNA extraction, which kit works best? - (reply: 6)
  420. Binding and eluting a DNA binding column - (reply: 1)
  421. genomic dna isolated from blood is brown color?! - (reply: 4)
  422. Removal of RNA from DNA sample - (reply: 15)
  423. 3-colour labeling for the single argon-ion laser? - DNA + 2 antibodies (reply: 1)
  424. Only DNA ladder , No desired band in PCR - (reply: 4)
  425. low total/input DNA levels in ChIP work-up - (reply: 7)
  426. plasmid dna isolation - (reply: 1)
  427. DNA and RNA contamination in RT PCR water controls - (reply: 2)
  428. Long PCR and genomic DNA isolation problems - (reply: 2)
  429. DNA supercoiling on agarose gel -> reference needed - (reply: 1)
  430. DNA methylation analysis - direct sequencing gives CT peaks - (reply: 1)
  431. DNA extraction from FFPE? - (reply: 2)
  432. MYCOPLASMALOGY - MYCOPLASMA DNA EXTRACTION (reply: 7)
  433. T4 DNA polymerisation - elongation or removal? (reply: 3)
  434. Whole Genome Methylation Analysis Feasibility - Methylation analysis on DNA from rare cell populations (reply: 3)
  435. DNA Ladder Issues - DNA Ladder Agarose Gel (reply: 5)
  436. compound that block DNA replication in human cell - (reply: 1)
  437. Isolation of genomic DNA from microbial consortia - (reply: 2)
  438. EXtraction of DNA bands from acrylamide gel - (reply: 4)
  439. do small pieces of DNA form a helix - (reply: 2)
  440. Large Circular DNA - (reply: 1)
  441. best method for DNA extraction - (reply: 3)
  442. heredity of DNA methylation - (reply: 2)
  443. purity of genomic DNA, low 260/230? - (reply: 1)
  444. long DNA amplification - (reply: 11)
  445. DNA Laddering - (reply: 1)
  446. gDNA pcr product as standard for absolute quantification? - (reply: 1)
  447. purification of a large amount of DNA - (reply: 3)
  448. DNA ligation - (reply: 2)
  449. global DNA methylation quiagen kit - (reply: 14)
  450. DNA binding/EMSA question - question about controls in DNA binding/EMSA (reply: 4)
  451. Concentration of DNA for Transformation - (reply: 1)
  452. Request Genomic DNA of Burkholderia mallei - possible: collaboration or exchange DNA with pseudomallei culture (reply: 8)
  453. Attaching a double stranded DNA to a surface - (reply: 1)
  454. Another band after plasmid purificaiton - ran the plasmid DNA on a gel (reply: 2)
  455. DNA Question (T/F) - (reply: 4)
  456. what primers should I use for DNA sequencing? please help - (reply: 2)
  457. what primers should I use for DNA sequencing?? - (reply: 3)
  458. DNase and PMA/Iono - (reply: 1)
  459. plasmid DNA lab question - (reply: 11)
  460. Rescue my DNA extraction - (reply: 1)
  461. denaturation of dna - (reply: 1)
  462. PCR using genomic DNA - (reply: 2)
  463. Using analytical grade reagents for DNA extraction - (reply: 6)
  464. SOS....Genomic DNA extraction - Extraction from gram positive bacillus stearothermophilus (reply: 8)
  465. Optimal stock concentration for gDNA storage - (reply: 1)
  466. Manual reverse primer design for bisulfite traeted DNA - (reply: 4)
  467. Using DNA in gel extract kept at -20ºC - (reply: 2)
  468. Help! Plasmid disappearing after mini-prep. - A260 shows good quality DNA, but nothing appears on gel. (reply: 3)
  469. Help with Phenol-Chloroform DNA Extraction - (reply: 3)
  470. Further DNA purification steps needed - Post DNAeasy Kit Purification (reply: 4)
  471. DNA yield estimation - (reply: 1)
  472. Testing primer specificity... - ...without gDNA of target organisms (reply: 6)
  473. Size of fusarium DNA - (reply: 1)
  474. size of fungal DNA - (reply: 3)
  475. Best way to desalt very salty, low yield DNA - (reply: 3)
  476. Source of DNA for a Reporter Construct - How to PCR the promoter region of interest? (reply: 1)
  477. weak ChIP signal - suggestions to increase the amount of DNA? (reply: 10)
  478. DNA methylated PCR - (reply: 2)
  479. Why can't genomic DNA be stored at 2-8 for long periods? - (reply: 10)
  480. Genomic DNA from Blood Kits - (reply: 2)
  481. Direct PCR (PCR without DNA extraction) - (reply: 2)
  482. DNA concentration and Genome - (reply: 1)
  483. DNA ladders on a northern blot - (reply: 1)
  484. Accidently heated up the wrong DNA. Damage? - (reply: 2)
  485. genomic DNA quality for genome walking - (reply: 2)
  486. Large Molecular weight DNA Extarction - (reply: 7)
  487. weak bands for methylated DNA - (reply: 4)
  488. Amplfication of fungal DNA - (reply: 8)
  489. No amplification in PCR using gDNA extracted from formailin fixed paraffin embed - (reply: 3)
  490. dna quantification and quality assesement - abs 260nm / abs 280 nm <0.5 (reply: 2)
  491. optimal template dna concentrations - (reply: 1)
  492. Volume of DNA to add to PCR Master Mix? - (reply: 1)
  493. how much DNA you need for real time pcr after chip - CHIP (reply: 1)
  494. RNA Purification - After DNase (reply: 3)
  495. How To Fix NaOH/Tris DNA Digest Error? - (reply: 1)
  496. DNA extraction with expired kit? - (reply: 1)
  497. protein/DNA Sequence Alignment - (reply: 1)
  498. PCR with genomic dna - (reply: 9)
  499. DNA Enrichment for Methylation Analysis - (reply: 7)
  500. Finding corresponding amino acid in DNA sequence - (reply: 1)
  501. Buffer for 5 Kb DNA fragments - (reply: 2)
  502. Random library using TOPO vector - random genomic DNA ligated into TOPO, false inserts? (reply: 3)
  503. Plasmid DNA sequence and map - PLASMID DNA (reply: 2)
  504. DNA isolation - parasite - Cryptosporidium DNA isolation (reply: 3)
  505. pcr doesnt show any band for DNa walking - (reply: 8)
  506. DNA polymerases for PCR-sequencing - (reply: 11)
  507. help-how to extract fluo-labeled DNA from agarose gel - (reply: 1)
  508. Integrity of treated DNA - Is quality of bisulfite treated DNA poor? (reply: 1)
  509. Qiagen DNAeasy blood&tissue DNA isolation kit - (reply: 1)
  510. 5-AZA, Reversing DNA methylation (epigenetic reactivation experiments) - (reply: 3)
  511. Best method to send DNA? - (reply: 4)
  512. protein precipitation with TCA for dna extraction? - (reply: 1)
  513. no DNA after IP - (reply: 1)
  514. Trouble with PCR on genomic tomato DNA (I've tried many fixes) - (reply: 5)
  515. Best way to extract plasmid from Mycobacteria - Extraction of DNA (reply: 7)
  516. purefication and amplification of serum free DNA - (reply: 1)
  517. dnase treatment before his-tag purification - (reply: 3)
  518. DNA extraction fron HSV-1 infected Vero cell - (reply: 2)
  519. Freezing DNA have effects on real-time PCR efficiencies - (reply: 1)
  520. low DNA yield after extraction - (reply: 1)
  521. protein precipitation without phenol/chloroform for dna extraction? - (reply: 1)
  522. Is gDNA digestion before bisulfite conversion important? - (reply: 5)
  523. Agarose: DNA/dye mixture doesn't stay in the gel? - Thanks for any and all help received! =] (reply: 12)
  524. DNA isolation from tissue !!SOS!! - (reply: 1)
  525. GC rich DNA sequencing - (reply: 3)
  526. Gemonic Library - Deletion/Reduction of Chloro/Mit DNA (reply: 1)
  527. Plasmid DNA isolation - neutralisation of DNA (reply: 5)
  528. transcription - quality of DNA (reply: 1)
  529. Sterilize DNA preps - (reply: 5)
  530. Transcription - Quality of DNA (reply: 1)
  531. DIG DNA PCR labeling problem - (reply: 6)
  532. Storage/Shipping of DNA at ambient temps - (reply: 2)
  533. how to get rid of DNA (in TE) contamination - protein and phenol removal (no kits please) (reply: 17)
  534. Trizol RNA/DNA extraction - (reply: 3)
  535. dna not running for electrophoresis - (reply: 9)
  536. nondenaturing PAGE to separate dna - (reply: 4)
  537. RNA isolation for real-time RT-PCR - forgot to inactivate the DNAse (reply: 4)
  538. failed PCR on DNA extract from blood - (reply: 6)
  539. Problem encountered when dosing my gDNA by spectrophotometry - Never experienced such thing before! (reply: 7)
  540. DNA methylation prediction in plant DNA - (reply: 2)
  541. Phenol - why different for DNA and RNA extraction? (reply: 9)
  542. concentration of dna - (reply: 3)
  543. DNA dissolved in DMSO instead of H2O - (reply: 1)
  544. PCR contamination with human DNA first and now no bands!!! - (reply: 4)
  545. Questions regarding bisulfite treatment of DNA - (reply: 2)
  546. Fragmented DNA doesn't appear as bands on agarose gel - (reply: 5)
  547. Questions about 260/230 when reading DNA conc. - (reply: 1)
  548. Genomic DNA contamination of RNA - (reply: 24)
  549. dna separation in native PAGE - (reply: 3)
  550. DNA extraction and PCR advice requested - PCR, Contamination, Low abundance targets (reply: 7)
  551. Problem with DNA extraction from 1% agarose Gel - No DNA using Qiagen Qiaek 2 and Spin column kits (reply: 9)
  552. How stable is methylated DNA? - (reply: 4)
  553. dna concentration - please help (reply: 5)
  554. volume of DNA required (PCr product ) in agarose gel - (reply: 11)
  555. Restriction endonuclease - cut only double stand DNA? (reply: 4)
  556. separation of dna fragments in agarose gel electrophoresis - (reply: 7)
  557. Primer Design - Design Primer with DNA Sequence (reply: 1)
  558. Diluting DNA - (reply: 1)
  559. Dry Plant leaf samples for DNA extraction - (reply: 1)
  560. DNase I pre-treatment - how can i be sure that it was efficient??? (reply: 2)
  561. PCR without DNA extraction! - (reply: 2)
  562. dna degradation - (reply: 4)
  563. Gel shift with large DNA fragments (>1 kb) - Can I still do EMSA? (reply: 1)
  564. Filtration DNA before transfection? - (reply: 4)
  565. Genomic DNA and PCR - (reply: 4)
  566. DNAse activity in room temperature - (reply: 3)
  567. Can DNA be solubilized in hydrochloric acid? - (reply: 2)
  568. about the total RNA isolation (trizol methods) and further Dnase I - (reply: 3)
  569. Presence / lack of introns in genomic DNA of related species - (reply: 2)
  570. DNA cloning - (reply: 4)
  571. freezing of E.coli culture prior DNA prep - (reply: 5)
  572. EZ DNA Methylation kit - (reply: 1)
  573. DNA storage @ room temp on filter paper - (reply: 2)
  574. DNase I digestion - how long the fragments are at the end??? (reply: 3)
  575. Vortexing genomic DNA, detrimental or not? - (reply: 8)
  576. Transfection efficiency vs. amount of plasmid DNA - (reply: 1)
  577. DNA amplification but no bands on agarose gel, GC rich product, Roche - DNA amplification but no bands/smear in agarose gel (reply: 5)
  578. Genomic DNA copy number qPCR - Help with control (reply: 8)
  579. Why were proteins favoured over DNA as the genetic material before 1940? - University Genetics Past EXAM Question (reply: 2)
  580. Sources of Contamination in plasmid DNA Mini-Preps - (reply: 10)
  581. Genomic DNA contamination in RNA sample - (reply: 2)
  582. Isolation DNA and protein from a single sample - (reply: 5)
  583. DNA extraction from saliva/buccal swabs - (reply: 5)
  584. DNase I digestion - check if it worked?? (reply: 4)
  585. elution of dna from gel - (reply: 5)
  586. Question about DNA electrophoresis - (reply: 1)
  587. EZ DNA methyaltion kit...trouble shooting - (reply: 4)
  588. Could You Suggest me A good KIt for DNA extraction from Yeast? - (reply: 1)
  589. DNA extraction trouble - (reply: 7)
  590. DNA vs Protein Stains - Are there any that don't stain BOTH? (reply: 1)
  591. Extraction of large size genomic DNA - (reply: 3)
  592. Colored DNA after phenol extraction - Strange color in aqueous phase and degraded DNA... (reply: 1)
  593. CHIP - DNA concentration after sonication - (reply: 6)
  594. DNA Question for TV Show - (reply: 4)
  595. Principles behind DNA RNA protein isolation and purification - (reply: 2)
  596. Phenol chloroform extraction of DNA - (reply: 3)
  597. Shrimp nuclease to eliminate genomic DNA - (reply: 7)
  598. gDNA or cDNA amplification in RT-PCR - (reply: 3)
  599. suggestions on the genomic DNA quality - (reply: 2)
  600. ChIP DNA quality - problem with ChIP-qPCR (reply: 5)
  601. Dnase digestion and PCR - (reply: 4)
  602. Why is it necessary to add Taq DNA polymerase last during PCR? - (reply: 8)
  603. Promoter and genomic DNA - (reply: 2)
  604. Genomic DNA - (reply: 9)
  605. is there DNA ? - how to know ? (reply: 8)
  606. DNA digestion - Restriction Enzymes (reply: 3)
  607. Problem with DNA purification from agarose gel - (reply: 3)
  608. T4 DNA Ligase & Restriction Enzyme Expression - I am looking to save money! (reply: 4)
  609. DNA extration of pieces of human hearts - (reply: 2)
  610. DNA concentration from ultraspectrophotometer is not accurate - (reply: 6)
  611. RNA appearance on 1.5% gel after DNase I digestion - (reply: 2)
  612. DNA precipitation - (reply: 5)
  613. DNA folding to form the loop - (reply: 2)
  614. DNA purification - long fragments wanted - (reply: 1)
  615. Unidentified DNA bands - (reply: 1)
  616. Any good way to remove DNase? - (reply: 5)
  617. DNA shearing with Misonix 4000 and microplates cup horn - (reply: 2)
  618. Accidentally digested DNA overnight - still OK? - (reply: 4)
  619. dna for real time - (reply: 3)
  620. Remove RNA after DNA mini-prep - (reply: 1)
  621. Confirm DNA enrichment of methylation array - (reply: 2)
  622. Capillary DNA Sequencing - (reply: 6)
  623. can we use one-step RT-PCR kits to amplify DNA? - (reply: 1)
  624. Why is Cleavage of DNA using restrcition enzyme more suitable for genetic engine - !! confused confused confused !! (reply: 4)
  625. How pure is my DNA sample?? - Confused-need help urgently please! (reply: 1)
  626. unmethylaten DNA control - ?????????? (reply: 3)
  627. Tips for genomic DNA extraction from gram -ve bacteria - (reply: 2)
  628. M.SssI treatment of DNA - (reply: 1)
  629. Gel purification of vector DNA - low yield - (reply: 26)
  630. DNA IDENTIFICATION - HOW TO TELL THE DIFFERENCE (reply: 14)
  631. DNA extraction methods - PCI extrction (reply: 3)
  632. Plant DNA extraction - (reply: 5)
  633. Seperate DNA spin columns - (reply: 3)
  634. DNA quantification - (reply: 5)
  635. Digest genomic (eukaryote) DNA before running PCR, is it necessary? - I do not get any band from PCR using genomic DNA and different primers (reply: 6)
  636. Problem with DIG High Prime DNA Labeling and Detection Starter Kit II - (reply: 2)
  637. PCR from a smear genomic DNA ? - (reply: 5)
  638. DNA/RNA into nucleotides - (reply: 6)
  639. Fast, cheap and dummy proof large scale DNA extraction? - (reply: 6)
  640. difference between a linear and circular plasmid/DNA for stable transfections - will a circular plasmid stably integrate into the genome? (reply: 4)
  641. Help with Purifying DNA Hairpin - (reply: 2)
  642. Reuse DNA spin column - (reply: 22)
  643. Does UV cause DNA fragmentation? - how long do I need to put cells under UV to see fragmentation? (reply: 12)
  644. Best storage temperature for samples - serum, protein, tissue, DNA, RNA - Archives (reply: 8)
  645. Does phenol extraction have effect on DNA methylation? - (reply: 4)
  646. Selection during Extraction: Plasmid or Genomic DNA? - (reply: 1)
  647. RNase free syringe for Qiagen DNase treatment, could not find any company provid - (reply: 2)
  648. gDNA purification - anything better than alcohol precipitation? (reply: 13)
  649. Effect of freezing on DNA methylation? - (reply: 6)
  650. DNA sequencing analysis - DNA sequencing analysis (reply: 1)
  651. DNA sequencing analysis - DNA sequencing analysis (reply: 11)
  652. qPCR quantification of ChIP DNA - (reply: 2)
  653. In vivo transfection of plasmid DNA, how? - intramuscular injection DNA (reply: 2)